Francisco Manuel Tortosa Gil

Myocardial glutathione alterations in acute coronary occlusion in the dog.

Glutathione (GSH) decreases in dog myocardium upon acute coronary occlusion when compared with sham-operated dogs. Total glutathione content (GSHeq = GSH + 2GSSG) remains unchanged throughout the experiment (6 h after surgery) in both sham- and acute coronary occlusion-operated dogs. GSSG and GSH/GSSG ratio increases and decreases respectively in all animals but tends to reach the normal value after 6 h in sham-operated dogs. Both parameters (GSSG and GSH/GSSG ratio) remain altered in acute coronary occlusion-operated ones. This alteration of glutathione status in ischemic myocardium is discussed.

Flow cytometric analysis of peroxidative activity in granulocytes from coronary and peripheral blood in acute myocardial ischemia and reperfusion in dogs: Protective effect of methionine

BACKGROUNDnMethionine has shown protective effects in experimental models of myocardial infarction and is highly reactive to oxidative compounds produced by polymorphonuclear leukocytes (PMN), which in turn have been associated with myocardial damage. We have investigated the effect of methionine administration on spontaneous leukocyte peroxidative activity in myocardial ischemia and reperfusion.nnnMETHODSnIn anesthetized dogs, with coronary occlusion (90 min) and reperfusion (90 min), PMN activation was measured by flow cytometric determination of H(2)O(2) with dihydrorhodamine 123, and correlated to hemodynamic parameters and infarct presence. To assess a possible direct effect of methionine, H(2)O(2) and superoxide were measured by flow cytometry in dog leukocyte suspensions following in vitro stimulation with f-MLP.nnnRESULTSnPMN peroxidative activity in saline-treated dogs increased significantly after coronary occlusion and after reperfusion. These changes were greater in coronary venous blood than in femoral blood. Methionine administration (150 mg/kg, i.v.) before occlusion totally suppressed PMN activation, both after occlusion and reperfusion.nnnCONCLUSIONSnPMN are promptly activated in myocardial ischemia, and methionine administration prevents such activation. However, methionine has no direct effect on spontaneous peroxidative activity, and f-MLP induced peroxidative activity. These in vivo effects of methionine, may additionally contribute to explain its protective role in experimental -788-877-7QQ8-8-7-88-8-8778--8Q78-----8--8-Q-7-Q7----- --------------8888 888888-7777777777777777777777777777777----------------888888888888888888 8877777--87--------8-----------------7-8888-887-----------8----8-8-87777 7777777------------------------------------------------------T7OW

Effect of Glutathione on Canine Myocardial Ischaemia Without Reperfusion

Abstract— The present study was to evaluate the effect of exogenous glutathione on myocardial damage resulting from permanent (no reperfusion) coronary ligation (3 or 6 h) in anaesthetized dogs. Haemodynamics, infarct size and myocardial glutathione content were determined. Erythrocyte superoxide dismutase (SOD) activity was also determined in coronary venous blood samples. Glutathione was administered by the intraperitoneal route, 100 mg kg−1 as initial dose given 5 min before coronary ligation, and successive doses of 25 mg kg−1 every 40 min throughout the study period. Saline‐treated dogs showed myocardial infarction, a decrease in myocardial glutathione content, and a transient increase in SOD activity. Three hours occlusion in glutathione‐treated dogs resulted in a small reduction of infarct size, and no changes in myocardial glutathione content and SOD activity. By contrast, administration of glutathione failed to reduce infarct size and failed to prevent myocardial glutathione decrease in dogs subjected to 6 h occlusion. These results indicate that exogenous glutathione is of minor beneficial effect for myocardial damage resulting from permanent coronary occlusion and suggest that endogenous glutathione has a limited role in protecting against myocardial ischaemia without reperfusion.

Contributions to the History of Psychology: CXIX. The Spanish Neurohistological School's Legacy: Cajal and Lorente De Nó

Santiago Ramón y Cajal made his mark in the history of science as a brilliant researcher of the histology of the nervous system. His bold efforts and the international recognition he received during his lifetime played an important role in the consolidation of the Spanish Neurohistological School which is composed of a large number of eminent disciples. Amongst these, and of outstanding repute, is Rafael Lorente de Nó whose research had a significant influence on the work of Canadian psychologist Donald O. Hebb and, consequently, on the development of neurophysiological theory. This paper analyses some of the contributions of Cajal and his disciple Lorente de Nó which formed the anatomical and neuropsychological framework of contemporary psychology.

Characteristics of monographic special issues in Ibero-American Psychology journals: visibility and relevance for authors and publishers

This paper aims to determine the existence of differential characteristics between monographic special issues and regular non-monographic issues published by psychology journals according to different bibliometric indicators. The materials studied consisted of a total of 1120 articles published in 10 Ibero-American psychology journals included in the JCRs from 2013 to 2015. The number of monographic articles was 286 and the non-monographic works were 834. The results indicate that the articles published in monographic special issue journal receive a higher number of citations and that their publication times are shorter. A greater presence of journal committee members as authors of the papers in monographic special issues was also observed, and the number of authors per paper was lower compared to articles published in non-monographs. As a conclusion, publishing papers in monographic special issues versus non-monographic in the reviewed journals has some advantages for both journals and authors, such as greater international visibility and shorter publication times.