Francisco Wallenstein

Bioavailable metals and cellular effects in the digestive gland of marine limpets living close to shallow water hydrothermal vents

The pressure exerted by shallow water hydrothermal vents on edible gastropods and their cellular responses triggered by these stresses are almost unknown. The aims of this study were to evaluate the bioavailability of metals in the Macaronesian endemic limpet Patella candei gomesii living close to shallow water hydrothermal vents, and the structural differences in their digestive gland as well as the levels of apoptosis in that organ. Limpets were sampled in four sites, two with the presence of hydrothermalism and the other two without it. Whole body concentrations of several metals (Ca, Cd, Cs, Co, Cu, Fe, Hg, Mg, Mn, Pb, Rb, Se, Sr, and Zn) were obtained, morphometry analysis of the digestive gland and TUNEL test for apoptosis were also performed. Results revealed that the presence of shallow water hydrothermal vents is a source of chronic metal stress to limpets, imposing modifications in the morphometry and cell composition of the digestive gland of those limpets that may constitute cell and tissue adaptations to the environment they live in. This study sets up new baseline data for further research on the influence of shallow water hydrothermal vents over communities living in these habitats.

Intertidal rocky shore biotopes of the Azores: a quantitative approach

Rocky littoral communities of the Azores are dominated by macroalgae where turf formations represent the main life form as on many subtropical and tropical shores. The present study aimed at describing and classifying benthic intertidal biotopes of the Azores using clear quantification and data analysis methodologies to be used in similar studies for spatial and/or temporal comparison. This numerical approach intends to have management and conservation applicability. Thirty study locations along the coast of São Miguel island were characterised by substratum type (cobbles, boulders or bedrock – different stability levels) and exposure to wave action (high, medium or low exposure), from June to August 2002. Algae, sessile and sedentary invertebrates were recorded along transects, and the more conspicuous taxa quantified. Community structure is mainly shaped by substratum stability – unstable cobble communities are separate from those of more stable boulders and bedrock. Boulders present an intermediate community composition between cobbles and bedrock. Exposure to wave action induces smaller variation in community composition. Sixteen biotopes have been recognised. Ultimately the present paper provides an objective set of biotope definition tools for a broad public whose interest focuses on spatial and temporal comparison of coastal communities and associated habitats.

Influence of a breakwater on nearby rocky intertidal community structure

It is widely recognised that coastal-defence structures generally affect the structure of the assemblages they support, yet their impact on adjacent systems has been largely ignored. Breakwaters modify the nearby physical environment (e.g. wave action) suggesting a local impact on biological parameters. In the present study, an ACI (After-Control-Impact) design was used to test the general hypothesis that the artificial sheltering of an exposed coast has a strong effect on the structure and functioning of adjacent systems. The effects of a reduction in hydrodynamics were clear for a number of taxa and included the replacement of barnacles, limpets and frondose algae by an increasing cover of ephemeral algae. These effects were evident both at early and late successional stages. Results suggest that the artificial sheltering of naturally exposed coasts can have a strong impact promoting a shift from consumer- to producer-dominated communities, which has important ecological and energetic consequences for the ecosystem.

Algae-based biotopes of the Azores (Portugal): spatial and seasonal variation

The increasing importance of coastal management created the need for a systematic classification and characterization of marine communities. Accurate quantitative methodologies for rocky shore algae-based biotope definition, were developed and tested on the Islands of São Miguel and Santa Maria (Azores). Shores of both islands were surveyed, covering all rocky substrate types. Biotopes were defined by assessing the associated habitat and species characteristics, using ANOSIM and SIMPER analysis, respectively. A total of ten biotopes were identified. Generally both islands’ biotopes are characterized by the same taxa/ecological categories, in summer and in winter. However, association between these taxa/ecological categories and the shore height at which they occur differs geographically and temporally. There is a generalized gradual succession of taxa/ecological categories from upper intertidal down to deepest subtidal, although geographical differences occur. Diversity is highest at the land–water interface and decreases towards both extremes (upper intertidal and deepest subtidal level). The strongest evidence of seasonal variation occurs at the upper intertidal. The methodology used proves to be effective in broad scale shoreline assessment of biological communities in warm-temperate coastal marine environments, and thus suitable for the purpose it was developed for. As a consequence it should be applied to the remaining islands of the Azorean archipelago as well as to other macaronesian islands, e.g. Madeira and the Canaries.

Subtidal Rocky Shore Communities of the Azores: Developing a Biotope Survey Method

Abstract At 23 sites selected randomly around the island of São Miguel (Azores), video records were made at the depths of 5, 10, 15, 20, 25, and 30 m where a rocky substratum was present. Semiquantitative abundance data of the most common benthic organisms (algae and fixed/sedentary macroinvertebrates) were recorded in the vicinity of each depth reference point. Qualitative samples of the more conspicuous organisms were taken for confirmation of identification in the laboratory. At each site, substratum type, geographical orientation, and depth level were recorded. Multivariate analysis (nonmetric multidimensional scaling; analysis of similarity [Anosim] tests; similarity percentages [Simper] analysis) on the collected data enabled patterns of community distribution to be identified, as well as their relationship to abiotic factors. Since depth was found to be the sole determining factor for communities, it was the only one considered in the subtidal biotope survey protocol proposed here.

Sampling strategies for biotope definition: minimal sampling area for selected groups of macroinvertebrates in the rocky subtidal of Sao Miguel, Azores

Azorean rocky shores are mainly characterized by patchy algae-based communities with variable associated macrofauna. Characterization studies should therefore include quantitative information for both algae and macroinvertebrates. Unlike for the algae, minimal sampling areas are undefined for macroinvertebrates in the Azores. The present study defines the minimal area to be used for the assessment of the abundance of conspicuous benthic macroinvertebrate abundance. This study proposes methodologies to be used for a selected group of invertebrates when simultaneously undertaking quantifications of macroalgae.

Intertidal rocky shore seaweed communities subject to the influence of shallow water hydrothermal activity in São Miguel (Azores, Portugal)

The volcanic origin of the Azores archipelago (Portugal) gives rise to active deep sea and shallow water hydrothermal activity that affects benthic communities. Intertidal seaweed surveys were conducted at two shores affected by intense shallow water hydrothermal vents. Water temperature, acidity and salinity were monitored. Seaweed communities were found to be species poor and have a disproportionally larger number of filamentous early successional species on shores that are subject to the effect of hot and acidic freshwater of volcanic origin. There is an ecological resemblance between hydrothermally affected seaweed communities in the Azores and those affected by acid mine drainage in the UK, thus indicating that hydrothermalism can be a useful scenario for pollution studies under conditions of ocean warming and acidification.

Shallow subtidal macroalgae in the North-eastern Atlantic archipelagos (Macaronesian region): a spatial approach to community structure

ABSTRACT Shallow subtidal macroalgal communities in the North-eastern Atlantic archipelagos (Azores, Madeira, Canaries and Cape Verde) were studied in order to identify their spatial organization patterns and the main drivers of change. Fifteen islands and 145 sites across 15º of latitude and 2850 km were sampled. We found high spatial variability across the scales considered (archipelago, island and site). The structure of macroalgal communities differed among archipelagos, except between Madeira and the Canaries, which were similar. Across a latitudinal gradient, macroalgal communities in the Azores were clearly separated from the other archipelagos; communities in Madeira and the Canaries occupied an intermediate position, while those in Cape Verde appeared at the opposite end of the gradient. In the Azores, species with warm-temperate affinities dominated communities. Cape Verde communities were, in contrast, dominated by tropical taxa, whereas in the subtropical Canaries and Madeira there was a mixture of species with colder and warmer affinities. Apart from crustose coralline algae, the Dictyotales were the group with greatest cover; larger and longer-lived species were progressively replaced by short-lived species along a latitudinal gradient from north to south. The perennial species Zonaria tournefortii dominated the sea-bottom in the Azores, the semi-perennial Lophophora variegata in the Canaries, the filamentous algae in Madeira and the ephemeral Dictyota dichotoma in Cape Verde. We hypothesized that the differences among archipelagos could be explained by synergies between temperature and herbivory, which increased in diversity southwards, especially in Cape Verde. This was supported by the predominance of non-crustose macroalgae in the Azores and of crustose macroalgae in Cape Verde, as would be predicted from the greater herbivore activity. At the scale of islands and sites, the same set of environmental variables drove differences in macroalgal community structure across all the Macaronesian archipelagos.