François J. Meunier

Osteoclasts in teleost fish: Light-and electron-microscopical observations

SummaryThis paper reports the common occurrence of osteoclasts during normal and experimental bone resorption in a number of teleost fishes. Light-microscopical observations on osteoclasts are presented in resorption areas on perichondral bone (mandibula and pharyngeal jaws of cichlids and vertebrae of gymnotids), on dermal bone (mandibula of salmonids and characoids and frontal bone of cichlids), on chondroid bone (pharyngeal jaws of cichlids), and on elasmoid body scales (eichlids and gymnotids). Osteoclasts acting along the bone surface usually lie in a Howships lacuna whereas others are wrapped around bone extremities. Electronmicroscopical observations reveal that teleost osteoclasts show features similar to those of higher vertebrate osteoclasts, c.g., the presence of a ruffled border and the occurrence of numerous vacuoles, lysosomes and mitochondria. The multinucleated aspect that characterizes osteoclasts in other vertebrate groups is not a distinct feature of teleost osteoclasts since some are possibly mononucleated. Teleost osteoclasts are also able to resorb uncalcified tissues adjoining bone resorption areas, either as a primary process directed toward the tissue (basal plate of elasmoid scale) or as a secondary phenomenon (cartilage).

Comparative study of lower pharyngeal jaw structure in two phenotypes of Astatoreochromis alluaudi (teleostei: Cichlidae)

The potentially molluscivorous East‐African cichlid Astatoreochromis alluaudi is known to exhibit phenotypic plasticity in its pharyngeal jaw apparatus. We examined wild‐caught (snail‐eating) fish and specimens experimentally reared on soft food for differences in bone structure in their lower pharyngeal jaw (LPJ). The LPJ is built up of two halves, each of which consists of four structural units: a bony dentigerous, sutural and cortical plate, surrounding a medullary cavity containing sparse bone. Histomorphometric data and associated statistical analysis on serial microradiographs through the posterior third region of the LPJ, where crushing forces are assumed to be the highest, reveal differing growth trajectories: (1) compensating for fish size (standard length) the LPJ grows to a significantly larger size and volume in snail‐eating specimens, (2) all structural units distinguished contribute to the volume increase of the LPJ in the hard versus the soft phenotype, and (3) the bone volume fraction in each of the units keeps pace with the growth of the unit proper, indicating that porosity does not change on one growth trajectory or from one phenotype to another. In addition, morphological observations show in hard food specimens: (1) the development of a structurally different bony layer along the inner side of the cortical plate, and (2) a reinforcement of the medullary cavity in the form of oriented trabeculae. Both are interpreted as a consolidation of the medullary cavity to resist the compressive forces exerted when hard food particles (mollusc shells) are crushed.

Middle Maastrichtian vertebrates (fishes, amphibians, dinosaurs and other reptiles, mammals) from Pajcha Pata (Bolivia). Biostratigraphic, palaeoecologic and palaeobiogeographic implications

The Pajcha Pata fossil locality in south central Bolivia, in the upper part of the Lower Member of the El Molino Formation, is the first late Cretaceous fauna in South America which has yielded, in addition to some invertebrates and plants, all kinds of vertebrates except birds, but including dinosaurs and mammals. Its middle Maastrichtian age, indicated by geochronologic data, is consistent with the fauna, including the marine ichthyofauna. The vertebrate fossils reported here come from the main fossiliferous level which includes terrestrial, freshwater and marine taxa: vertebrates, Mammalia (both tribosphenic and non-tribosphenic therians), Theropoda (Coelurosauria and Sauropoda), Crocodylia, Squamata (Serpentes), Chelonia, Amphibia (Anura, Gymnophiona, Caudata) and fish (Dipnoi, Teleostei, Actinopteri, Cladistia, Chondrostei); invertebrates (Gastropoda, Bryozoa) and plants (charophytes). Amongst these taxa are the earliest records of some fish, Amphibia and tribosphenic Mammalia in South America and/or in the world. The fish concerned are: Polypteriformes (Latinopollia suarezi), Siluriformes of the family Andinichthyidae (Andinichthys) and two new families, Osteoglossiformes of the subfamily Heterotidinae (Osteoglossidae), Perciformes of the family Latidae, Dipnoi of the family Lepidosirenidae (Lepidosiren cf. paradoxa). The Amphibia concerned are: indeterminate Gymnophiona, Noterpetontidae (Noterpeton bolivianum). Pajcha Pata is the only known locality in South America with both non-tribosphenic and tribosphenic therian mammals. The depositional environment was probably estuarian or lagoonal as indicated by a mixed continental, freshwater, and marine fauna. Comparison of this local fauna with faunas of the same age at localities belonging to the same proximal part of the El Molino basin on one hand, and with others belonging to the distal part on the other hand, shows that the two continental areas seem to have their own endemic freshwater fish fauna (except the Characidae and the Lepisosteidae, known in all levels of Bolivia). However, these two areas have the same marine taxa. This implies some influence of the sea in the whole basin. Calculated temperatures of the marine waters range from 13 to 17° for a latitute of about 22°S, implying a southern Pacific upwelling. The El Molino Basin could have been also connected with the open sea through present-day Argentina and not only Venezuela as thought until now.

The concept of bone tissue in Osteichthyes

The purpose of this paper is to highlight the difficulties encountered when attempting to give a histological definition of bone tissue in Osteichthyes. Although the three basic components of bone tissue can be present (i.e. osteocytes, an organic matrix, and a mineral phase), it has long been known that bony tissues in Osteichthyes can lack trapped cells and/or mineral. This phenomenon has blurred the classical distinction between the generally adopted categories of connective tissues in a way that the osteichthyan skeleton should be described rather in terms of a continuum of structures. This paper illustrates this by discussing the evolutionary trends, within the Osteichthyes, of acellularization (i.e. the acquisition of acellular bone within the various osteichthyan lineages) and of loss of capacity of mineralization (e.g. in the case of isopedine of the basal plate of elasmoid scales). A further example of the difficulty of classifying skeletal tissues within bony fishes is provided by chondroid bone, a tissue with characteristics intermediate between cartilage and bone and found mostly in articular areas in the head of Teleostei. Each of the bone and bone-derived tissues of the aforementioned continuum represents the outcome of developmental and functional constraints, which appear to be more diverse in Osteichthyes than in Tetrapoda.

Demineralization of the vertebral skeleton in Atlantic salmon Salmo salar L. during spawning migration.

In Atlantic salmon, Salmo salar, the mineral rate of vertebrae in a given fish varies according to the position of the vertebra along the rachidian axis. Indeed, the mean rate goes from 49% in the anterior vertebrae and raises to 51% in post-truncal vertebrae. Although no significant difference in the mineral rate was noticed between males and females either in the lower river basin or after spawning, the mineral rate of vertebral bone decreased significantly (1-2%) during spawning migration. Vertebrae, like scales, are an important reservoir of calcium from which fasting salmon draws the minerals and organic materials necessary for the substantial remodeling of cranial bones in males and for sexual maturation. We hypothesize that mineral decrease in vertebrae may be the result of a halastasic demineralization of the vertebral tissues.

Morphological screening of carp Cyprinus carpio: relationship between morphology and fillet yield

Abstract We propose a simple method to quantify the morphology of 2–3-year-old carps ( Cyprinus carpio ) which is related to their fillet yield. This method is based on the automated image analysis of the lateral projection of the fish (mask). Seven morphological measurements specifically related to fillet yield of carps were defined. The comparison between this method and the conventional quantification offish shape, based on measurements of skeletal landmarks, increases the significance of this fitting. From the analysis of the morphometric data, we propose a profitable carp morphotype which is defined by four angles and two distances which quantify the shape of the head and the position of the caudal peduncle, respectively. The main characteristic of this morphotype is the absence of a large dorsal development, and the ventral position of the caudal peduncle.

Diversification in Polypteriformes and Special Comparison With the Lepisosteiformes

Polypteriformes (or Cladistia) and Lepisosteiformes (or Ginglymodi) are two groups of freshwater fishes with ganoid scales. The earliest fossil records of these taxa are Albian (Lepisosteiformes) and Cenomanian (Polypteriformes) respectively in Gondwana; they are still extant. The ‘first’ appearance of the two groups in the fossil record (explosive in polypteriforms, gradual in lepisosteiforms) as well as their evolutionary mode (diversification/disparity or replacement) is described in detail. The lepisosteiforms appear to show a rapid radiation of post-Palaeozoic clades immediately upon origination, while the polypteriforms represent a counter-example with their sudden diversification and their sudden acquisition of several ‘key innovations’.

A microanatomical and histological study of the postcranial dermal skeleton in the Devonian sarcopterygian Eusthenopteron foordi

The fin rays and two types of scales (enlarged and regular) of the Devonian sarcopterygian Eusthenopteron foordi are redescribed using light, scanning and transmission electron microscopy. The fin rays consist of lepidotrichia composed of ossified, jointed and branched segment pairs. The basal segments are cylindrical, but more distal elements are crescentic in section. The distribution of Sharpeys fibres varies along the lepidotrichia. In the proximal segments, lateral bundles form a belt connecting adjacent hemisegments. In the distal segments, thin bundles are restricted to the area facing the fin surface. Both enlarged and regular scales have a similar spatial organisation. They are composed of a superficial highly mineralised layer covering a thick basal plate where the fibrils are distributed in superimposed strata forming a plywood-like structure. Nevertheless, the enlarged and regular scales differ in their shape, in the mineralised tissues of the superficial layer, and in the organisation of the plywood-like structure. The superficial layer of the enlarged scales is composed of parallel-fibered bone covering a deeper layer of woven-fibered bone. The basal plate is made of an orthogonal plywood-like structure. The thin, lamellar, imbricated regular scales display the characteristics of elasmoid scales. The mineralised tissue forming the superficial layer resembles that of extant teleost scales. In the basal plate, the twisted plywood-like structure is composed of closely packed fibrils that are preserved down to the ultrastructural level owing to the persistence of bridges connecting the fibrils. The enlarged and the regular scales of Eusthenopteron foordi do not present superficial odontodes, in contrast to ancestral thick rhomboid scales. The disappearance of enamel/enameloid and dentine may be related to the evolutionary trend towards a lightening of the dermal skeleton that would improve the swimming abilities of the animal. The characteristics of the dermal skeleton of Eusthenopteron foordi support the hypothesis that this process began early in osteichthyans.

Experimental regeneration of the caudal skeleton of the glass knifefish, Eigenmannia virescens (Rhamphichthydae, Gymnotoidei)

This paper describes the regeneration of the caudal axial skeleton after amputation of the tail, including about 20 vertebrae, in the gymnotoid fish Eigenmannia virescens. Seven days after amputation, a regeneration blastema developed and soft tissues degenerated. A cylinder of cartilage developed at the end of the notochord. When this cartilage was about 10 mm long (21 days), perichondral ossification began. The cartilage continued to elongate and ossification increased while osteoclasts began to destroy the cartilage ventrally. Finally, a bony rod formed and at its tip the cartilage persisted as a rod, 2 to 3 mm long. The anal fin also regenerated: Endoskeletal cartilage developed first, following by differentiation of the lepidotrichia, and finally ossification of the endoskeleton.

Cortisol mobilizes mineral stores from vertebral skeleton in the European eel: an ancestral origin for glucocorticoid-induced osteoporosis?

Endogenous excess cortisol and glucocorticoid (GC) therapy are a major cause of secondary osteoporosis in humans. Intense bone resorption can also be observed in other vertebrates such as migratory teleost fish at the time of reproductive migration and during fasting when large amounts of calcium and phosphate are required. Using a primitive teleost, the European eel, as a model, we investigated whether cortisol could play an ancestral role in the induction of vertebral skeleton demineralization. Different histological and histomorphometric methods were performed on vertebral samples of control and cortisol-treated eels. We demonstrated that cortisol induced a significant bone demineralization of eel vertebrae, as shown by significant decreases of the mineral ratio measured by incineration, and the degree of mineralization measured by quantitative microradiography of vertebral sections. Histology and image analysis of ultrathin microradiographs showed the induction by cortisol of different mechanisms of bone resorption, including periosteocytic osteolysis and osteoclastic resorption. Specificity of cortisol action was investigated by comparison with the effects of sex steroids. Whereas, testosterone had no effect, estradiol induced vertebral skeleton demineralization, an effect related to the stimulated synthesis of vitellogenin (Vg), an oviparous specific phospho-calcio-lipoprotein. By contrast, the cortisol demineralization effect was not related to any stimulation of Vg. This study demonstrates GC-induced bone demineralization in an adult non-mammalian vertebrate, which undergoes natural bone resorption during its life cycle. Our data suggest that the stimulatory action of cortisol on bone loss may represent an ancestral and conserved endocrine regulation in vertebrates.