François J. Tardif

Mutations in ALS confer herbicide resistance in redroot pigweed (Amaranthus retroflexus ) and Powell amaranth (Amaranthus powellii )

Abstract A number of redroot pigweed and Powell amaranth populations from various locations in Ontario, Canada, have distinct patterns of resistance to the acetolactate synthase–inhibiting herbicides imazethapyr and thifensulfuron. This suggested the presence of diverse ALS gene mutations among these populations. Seven polymerase chain reaction primer pairs were used to amplify the gene to obtain full sequence information and to determine the identity of resistance-conferring mutations. There was a high degree of similarity in the ALS gene of the two species with only five nucleotides and one amino acid differing. A total of four herbicide resistance-conferring mutations were identified in the two species. The Ala122Thr, Ala205Val, and Trp574Leu amino acid substitutions were found in redroot pigweed whereas Ala122Thr, Trp574Leu, and Ser653Thr were detected in Powell amaranth. The pattern of resistance known to be conferred by the mutations concurred with the resistance level observed at the whole plant level. Distinct mutations being found in geographically separated populations suggest that selection for resistance occurred simultaneously in different locations. It reinforces the fact that resistance to ALS inhibitors is easily selected and that growers need to take this into account when formulating weed management strategies. Nomenclature: Imazethapyr; thifensulfuron; Powell amaranth, Amaranthus powellii S. Wats. AMAPO; redroot pigweed, Amaranthus retroflexus L. AMARE.

The Biology of Invasive Alien Plants in Canada. 3. Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea & Tardif

This annual dioecious weed was found in 2002 and 2003 infesting soybean fields in southwestern Ontario, and it was collected in 1992 from waste places in British Columbia. It is a major weed problem in field crops in the mid-western United States, where it has become increasingly difficult to control during the past 10 yr. Morphological differences between Amaranthus tuberculatus var. rudis and var. tuberculatus are presented. A review of the biological information published is provided. Plants exhibit high phenotypic plasticity and genetic variability. Emergence is prolonged, growth rapid, and female plants produce a large number of viable seeds that contribute to a persistent seed bank. Amaranthus tuberculatus var. rudis has developed multiple resistance to triazine and acetolactate synthase- and protoporphyrinogen-inhibiting herbicides. Airborne pollen can travel significant distances and A. tuberculatus var. rudis may hybridize with other noxious Amaranthus spp. transferring herbicide resistance or ot...

The biology of Canadian weeds. 133. Cuscuta campestris yuncker, C. gronovii willd. ex Schult., C. umbrosa beyr. ex hook., C. epithymum (L.) L. and C. epilinum Weihe

Cuscuta spp. (dodders) are rootless, holoparasitic herbs with filiform stems attached to the host by numerous haustoria. In Canada, Cuscuta gronovii is the most common native species of the genus followed by Cuscuta campestris and C. umbrosa. Cuscuta epithymum and C. epilinum, both introduced species in Canada, occur occasionally. Infestation by Cuscuta spp. can result in serious yield losses and dodders are listed as noxious weeds in British Columbia, Ontario and Quebec, and as restricted weeds in Alberta. These plants have evolved special adaptations to ensure their success: germination occurs late in the season when potential hosts are already established; seedlings selectively forage in plant communities and they may survive relatively long periods during the autotrophic stage. Invasion occurs via extremely elaborate mechanisms designed to match the biological processes of their host and bypass defense mechanisms. The principal means of dispersal of Cuscuta weeds world-wide (including Canada) has been...

An Ala205val Substitution in Acetohydroxyacid Synthase of Eastern Black Nightshade (Solanum Ptychanthum) Reduces Sensitivity to Herbicides and Feedback Inhibition

Abstract Twelve populations of eastern black nightshade from different locations in Ontario are resistant to imazethapyr. This study aimed at determining the molecular basis of resistance in these populations and the activity of the resistant acetohydroxyacid synthase (AHAS) enzyme compared to that of the sensitive AHAS in response to different herbicides and branched-chain amino acid concentration. The results of partial AHAS sequencing indicated that all resistant populations had a cytosine331 to thymine substitution coding for an alanine205 to valine substitution. In vitro AHAS enzyme assays of one resistant population showed that the specific activity of the resistant enzyme was 56% less than that of the susceptible enzyme. AHAS from the resistant population was 72-, 70-, and 64-fold less sensitive than that of the susceptible population to imazethapyr, imazamox, and primisulfuron, respectively. Furthermore, the resistant enzyme was less sensitive to feedback inhibition from branched-chain amino acids compared to the susceptible enzyme. Results confirmed that resistance in resistant populations of eastern black nightshade was conferred by target-site modification and that the Ala205Val substitution alters the kinetics and regulation of branched-chain amino acid biosynthesis. Nomenclature: Imazethapyr; imazamox; primisulfuron; eastern black nightshade, Solanum ptychanthum Dunal SOLPT.

Multiple resistance to imazethapyr and atrazine in Powell amaranth (Amaranthus powellii)

Abstract Multiple-herbicide resistance represents an added weed management challenge to growers as it can considerably reduce their options for weed control. The widespread nature of triazine resistance in Ontario coupled with the more recent appearance of resistance to ALS inhibitors in Amaranthus species warranted documenting biotypes with multiple resistance. A collection of Powell amaranth and redroot pigweed biotypes that had previously been characterized for resistance to ALS inhibitors was therefore screened with atrazine. Dose–response analysis with atrazine and imazethapyr was also conducted. High-level resistance to imazethapyr and atrazine was determined in a Powell amaranth biotype from Perth County, Ontario. This biotype had a > 1,860-fold and 109-fold resistance to atrazine and imazethapyr, respectively. Sequence analysis was conducted for the psbA and ALS genes that code for the target sites of the triazines and imidazolinones, respectively. A mutation in the psbA gene was identified that coded for an amino acid substitution of glycine for serine at residue 264 of the D1 protein. This mutation is the most likely cause for triazine resistance in this biotype. Similarly, a nucleotide substitution was identified that codes for threonine in place of serine at position 652 of the ALS protein. This mutation in the ALS gene has only been observed previously in laboratory-selected mutants of arabidopsis and tobacco and is known to endow resistance to imidazolinones in plants. It is concluded that multiple resistance in this Powell amaranth biotype is due to the presence of altered target sites for triazine and imidazolinone herbicides. Nomenclature: Atrazine; imazethapyr; Powell amaranth, Amaranthus powellii S. Wats. AMAPO; redroot pigweed, Amaranthus retroflexus L. AMARE; Arabidopsis thaliana (L.) Heynh. Arabidopsis; tobacco, Nicotiana tabacum L.

Weed control in glufosinate-resistant corn (Zea mays).

Abstract: The development of glufosinate-resistant corn represents a new weed management system for corn growers. Field experiments were conducted from 1995 to 1997 at four locations in southwestern Ontario. The objective of this study was to determine the effect of timing of weed control relative to the growth stage of corn with glufosinate applied alone or in combination with residual herbicides. Control of all species tested improved with the addition of atrazine plus dicamba to glufosinate, applied from the two- to eight-leaf stage of corn growth. Based on a 90% weed dry matter reduction, glufosinate with atrazine plus dicamba controlled common ragweed, common lambsquarters, and pigweed species at the three-leaf stage of corn and yellow foxtail, barnyardgrass, and large crabgrass at the two-, four-, and eight-leaf stage of corn, respectively. Weed control with glufosinate alone was improved when applied at the later growth stages of corn. Glufosinate applied alone at the four-leaf stage of corn controlled common ragweed and common lambsquarters, whereas pigweed species were controlled effectively at the eight-leaf stage of corn growth. Corn grain yield was consistently higher when glufosinate was applied in combination with residual herbicides, compared to glufosinate alone. Glufosinate in combination with residual herbicides applied to corn at the three- to five-leaf stage may represent the best timing for weed control. Our data suggested that a tank mixture of glufosinate with other postemergence residual herbicides or a split application of glufosinate in combination with cultivation may be required for weed control in glufosinate-resistant corn. Nomenclature: Atrazine; dicamba; SAN 582 (proposed name, dimethenamid), 2-chloro-N-[(1-methyl-2-methoxy)ethyl]-N-(2,4-dimethyl-thien-3-yl)-acetamide; glufosinate; metolachlor; redroot pigweed, Amaranthus retroflexus L. #3 AMARE; common lambsquarters, Chenopodium album L. # CHEAL; yellow foxtail, Setaria glauca (L.) Beauv. # SETGL; large crabgrass, Digitaria sanguinalis (L.) Scop. # DIGSA; barnyardgrass, Echinochloa crus-galli (L.) Beauv. # ECHCR; common ragweed, Ambrosia artemisiifolia L. # AMBEL; corn, Zea mays L. Additional index words: Integrated weed management. Abbreviations: DAE, days after emergence; DM, dry matter; HRC, herbicide-resistant crops; IWM, integrated weed management; POST, postemergence; PRE, preemergence.

Glyphosate-Resistant Giant Ragweed (Ambrosia trifida) Control in Dicamba-Tolerant Soybean

Abstract Glyphosate-resistant (GR) giant ragweed has been confirmed in Ontario, Canada. Giant ragweed is an extremely competitive weed and lack of control in soybean will lead to significant yield losses. Seed companies have developed new herbicide-resistant (HR) crop cultivars and hybrids that stack multiple HR traits. The objective of this research was to evaluate the efficacy of glyphosate and glyphosate plus dicamba tank mixes for the control of GR giant ragweed under Ontario environmental conditions in dicamba-tolerant (DT) soybean. Three field trials were established over a 2-yr period (2010 and 2011) on farms near Windsor and Belle River, ON. Treatments included glyphosate (900 g ae ha−1), dicamba (300 g ae ha−1), and dicamba (600 g ha−1) applied preplant (PP), POST, or sequentially in various combinations. Glyphosate applied PP, POST, or sequentially provided 22 to 68%, 40 to 47%, and 59 to 95% control of GR giant ragweed and reduced shoot dry weight 26 to 80%, 16 to 50%, and 72 to 98%, respectively. Glyphosate plus dicamba applied PP followed by glyphosate plus dicamba applied POST consistently provided 100% control of GR giant ragweed. DT soybean yield correlated with GR giant ragweed control. This is the first report in Canada of weed control in DT soybean, specifically for the control of GR giant ragweed. Results indicate that the use of dicamba in DT soybean will provide an effective option for the control of GR giant ragweed in Ontario. Nomenclature: dicamba; glyphosate; giant ragweed, Ambrosia trifida L.; soybean, Glycine max (L.) Merr.

Multiple allelic forms of acetohydroxyacid synthase are responsible for herbicide resistance in Setaria viridis

In weed species, resistance to herbicides inhibiting acetohydroxyacid synthase (AHAS) is often conferred by genetic mutations at one of six codons in the AHAS gene. These mutations provide plants with various levels of resistance to different chemical classes of AHAS inhibitors. Five green foxtail [Setaria viridis (L.) Beauv.] populations were reported in Ontario with potential resistance to the AHAS-inhibiting herbicide imazethapyr. The objectives of this study were to confirm resistance, establish the resistance spectrum for each of the five populations, and determine its genetic basis. Dose response curves were generated for whole plant growth and enzyme activity, and the AHAS gene was sequenced. Resistance was confirmed by determining the resistance factor to imazethapyr in the five resistant green foxtail populations for whole plant dose response experiments (21- to 182-fold) and enzyme assays (15- to 260-fold). All five imazethapyr-resistant populations showed cross-resistance to nicosulfuron and flucarbazone while only three populations had cross-resistance to pyrithiobac. Sequence analyses revealed single base-pair mutations in the resistant populations of green foxtail. These mutations were coded for Thr, Asn, or Ile substitution at Ser653. In addition, a new mutation was found in one population that coded for an Asp substitution at Gly654. There is an agreement between the spectra of resistance observed and the type of resistance known to be conferred by these substitutions. Moreover, it indicates that, under similar selection pressure (imazethapyr), a variety of mutations can be selected for different populations, making the resistance pattern difficult to predict from herbicide exposure history.

ALS inhibitor resistance in populations of Powell amaranth and redroot pigweed

Abstract In 1997, farmers in Ontario, Canada, reported failure of some ALS-inhibiting herbicides to provide adequate control of pigweed species. Growth room experiments were conducted to confirm resistance to ALS inhibitors in populations of Powell amaranth and redroot pigweed. Twenty-two out of 35 collected seed samples were able to grow in the presence of soil-applied imazethapyr or flumetsulam. Dose–response curves were generated for 11 and 9 populations of Powell amaranth and redroot pigweed, respectively, using foliar-applied imazethapyr and thifensulfuron. Resistance to ALS inhibitors was confirmed in nine and five populations of Powell amaranth and redroot pigweed, respectively. Within each species, comparison of the herbicide rate required to reduce plant dry weight 50% (GR50) between the resistant populations and a susceptible population was conducted to obtain resistance factors. For imazethapyr, resistance factors ranged from 4.2 to 3,438 and from 33 to 168 for Powell amaranth and redroot pigweed, respectively. High-level cross-resistance to thifensulfuron was found in two populations of each species, with resistance factors ranging from 270 to 2,416. In both species, populations could be grouped according to their cross-resistance patterns: some populations were resistant to imazethapyr only, whereas others expressed resistance to both imazethapyr and thifensulfuron. The observed patterns of cross-resistance were not correlated with known herbicide exposure history of the fields where these populations originated. Nomenclature: Flumetsulam; imazethapyr; thifensulfuron; Powell amaranth = green pigweed, Amaranthus powellii S. Wats. AMAPO; redroot pigweed, Amaranthus retroflexus L. AMARE.

Evaluation of site-specific weed management using a direct-injection sprayer

Abstract Targeting weed patches for site-specific herbicide applications potentially represents cost savings for operators, reduction in environmental herbicide effects, and increased efficiency of weed control. An experiment was initiated in a no-till corn field in Ontario, Canada, in 1998 and was continued in rotation with no-till soybeans in 1999. Weeds were intensively scouted, and distribution maps of the most common weeds (field horsetail, spiny sowthistle, dandelion, and common lambsquarters) were generated for both years. A prescription map for each plot was made using the weed density maps. Treatment decisions were based on a weed threshold value of 1 shoot m−2. Four herbicide treatments were compared: a conventional broadcast, a site-specific application targeting weed patches only, and two combinations of broadcast and site-specific applications. Treatments were applied using a direct-injection sprayer. Efficacy of weed control and yield were compared among treatments. In 1998 and 1999 there were no differences in the level of weed control or yield among treatments. The average percent area sprayed was reduced as much as 26% in the site-specific treatment in 1998 and up to 59% in the site-specific and broadcast combination treatments in 1999. For those species present in the field, patches ranged from highly aggregated to completely random, and patch stability ranged from very stable to very unstable over the 2 yr. Nomenclature:Common lambsquarters, Chenopodium album L. CHEAL; corn, Zea mays L.; dandelion, Taraxacum officinale Weber. TAROF; field horsetail, Equisetum arvense (L.) EQUAR; soybean, Glycine max (L.) Merr.; spiny sowthistle, Sonchus asper (L.) Hill. SONAS.