Frank B. Jaworski

Next-generation in vivo optical imaging with short-wave infrared quantum dots

For in vivo imaging, the short-wavelength infrared region (SWIR; 1000–2000 nm) provides several advantages over the visible and near-infrared regions: general lack of autofluorescence, low light absorption by blood and tissue, and reduced scattering. However, the lack of versatile and functional SWIR emitters has prevented the general adoption of SWIR imaging by the biomedical research community. Here, we introduce a class of high-quality SWIR-emissive indium-arsenide-based quantum dots (QDs) that are readily modifiable for various functional imaging applications, and that exhibit narrow and size-tunable emission and a dramatically higher emission quantum yield than previously described SWIR probes. To demonstrate the unprecedented combination of deep penetration, high spatial resolution, multicolor imaging and fast-acquisition-speed afforded by the SWIR QDs, we quantified, in mice, the metabolic turnover rates of lipoproteins in several organs simultaneously and in real time as well as heartbeat and breathing rates in awake and unrestrained animals, and generated detailed three-dimensional quantitative flow maps of the mouse brain vasculature.

Efficient luminescent down-shifting detectors based on colloidal quantum dots for dual-band detection applications.

A colloidal quantum dot (QD) luminescent down-shifting (LDS) layer is used to sensitize an InGaAs short wavelength infrared photodetector to the near UV spectral band. An average improvement in the external quantum efficiency (EQE) from 1.8% to 21% across the near UV is realized using an LDS layer consisting of PbS/CdS core/shell QDs embedded in PMMA. A simple model is used to fit the experimental EQE data. A UV sensitive InGaAs imaging array is demonstrated and the effect of the LDS layer on the optical resolution is calculated. The bandwidth of the LDS detector under UV illumination is characterized and shown to be determined by the photoluminescence lifetime of the QDs.

Review of short-wave infrared spectroscopy and imaging methods for biological tissue characterization

Abstract. We present a review of short-wave infrared (SWIR, defined here as ∼1000 to 2000 nm) spectroscopy and imaging techniques for biological tissue optical property characterization. Studies indicate notable SWIR absorption features of tissue constituents including water (near 1150, 1450, and 1900 nm), lipids (near 1040, 1200, 1400, and 1700 nm), and collagen (near 1200 and 1500 nm) that are much more prominent than corresponding features observed in the visible and near-infrared (VIS-NIR, defined here as ∼400 to 1000 nm). Furthermore, the wavelength dependence of the scattering coefficient has been observed to follow a power-law decay from the VIS-NIR to the SWIR region. Thus, the magnitude of tissue scattering is lower at SWIR wavelengths than that observed at VIS or NIR wavelengths, potentially enabling increased penetration depth of incident light at SWIR wavelengths that are not highly absorbed by the aforementioned chromophores. These aspects of SWIR suggest that the tissue spectroscopy and imaging in this range of wavelengths have the potential to provide enhanced sensitivity (relative to VIS-NIR measurements) to chromophores such as water and lipids, thereby helping to characterize changes in the concentrations of these chromophores due to conditions such as atherosclerotic plaque, breast cancer, and burns.

Quantitative short-wave infrared multispectral imaging of in vivo tissue optical properties

Extending the wavelength range of spatial frequency domain imaging (SFDI) into the short-wave infrared (SWIR) has the potential to provide enhanced sensitivity to chromophores such as water and lipids that have prominent absorption features in the SWIR region. Here, we present, for the first time, a method combining SFDI with unstructured (zero spatial frequency) illumination to extract tissue absorption and scattering properties over a wavelength range (850 to 1800 nm) largely unexplored by previous tissue optics techniques. To obtain images over this wavelength range, we employ a SWIR camera in conjunction with an SFDI system. We use SFDI to obtain in vivo tissue reduced scattering coefficients at the wavelengths from 850 to 1050 nm, and then use unstructured wide-field illumination and an extrapolated power-law fit to this scattering spectrum to extract the absorption spectrum from 850 to 1800 nm. Our proof-of-principle experiment in a rat burn model illustrates that the combination of multispectral SWIR imaging, SFDI, and unstructured illumination can characterize in vivo changes in skin optical properties over a greatly expanded wavelength range. In the rat burn experiment, these changes (relative to normal, unburned skin) included increased absorption and increased scattering amplitude and slope, consistent with changes that we previously reported in the near-infrared using SFDI.

Multispectral imaging via luminescent down-shifting with colloidal quantum dots

The high infrared quantum yield, continuous absorption spectrum, and band edge tunability of colloidal quantum dots (QD) has opened up new opportunities to use luminescent down shifting for multispectral imaging in the infrared. We demonstrate a QD sensitized short wavelength infrared (SWIR) camera which is capable of UV-SWIR multispectral imaging. The application of multispectral cameras for UV tagging applications is demonstrated and the extension of this technology to the mid infrared spectral region is discussed.

Large format high-operability SWIR and MWIR focal plane array performance and capabilities

High-performance large-format detector arrays responsive to the 1-5μm wavelength range of the infrared spectrum fabricated using large area HgCdTe layers grown on 6-inch diameter (211) silicon substrates are available for advanced imaging applications. This paper reviews performance and capabilities of Raytheon Vision Systems (RVS) HgCdTe/Si Focal Plane Arrays (FPA) and shows 2k x 2k format MWIR HgCdTe/Si FPA performance with NEdT operabilities better than 99.9%. SWIR and MWIR detector performance for HgCdTe/Si is comparable to established performance of HgCdTe/CdZnTe wafers. HgCdTe devices fabricated on both types of substrates have demonstrated very low dark current, high quantum efficiency and full spectral band fill factor characteristic of HgCdTe. HgCdTe has the advantage of being able to precisely tune the detector cutoff via adjustment of the Cd composition in the MBE growth. The HgCdTe/Si detectors described in this paper are p-on-n mesa delineated architecture and fabricated using the same mature etch, passivation, and metallization processes as our HgCdTe/CdZnTe line. Uniform device quality HgCdTe epitaxial layers and application of detector fabrication processes across the full area of 6-inch wafers routinely produces high performing detector pixels from edge to edge of the photolithographic limits across the wafer, offering 5 times the printable area as costly 6×6cm CdZnTe substrates. This 6-inch HgCdTe detector wafer technology can provide applications demanding very wide FOV high resolution coverage the capability to produce a very large single piece infrared detector array, up to a continuous image plane 10×10 cm in size. Alternatively, significant detector cost reduction through allowing more die of a given size to be printed on each wafer is possible, with further cost reduction achieved through transition towards automated detector fabrication and photolithographic processes for both increased yields and reduced touch labor costs. RVS continues to improve its FPA manufacturing line towards achieving low cost infrared FPAs with the format, size, affordability, and performance required for current and future infrared applications.

Dual-band ultraviolet-short-wavelength infrared imaging via luminescent downshifting with colloidal quantum dots

Abstract. The performance of short-wavelength infrared (SWIR) cameras in the visible and ultraviolet (UV) regions is limited by the absorption of high-energy photons in inactive regions of the imaging array. Dual-band UV-SWIR imaging can be achieved by using PbS colloidal quantum dots (CQD) to downshift incident UV light to the SWIR band. The CQD downshifting layer has minimal impact on the SWIR imaging performance and greatly increases the UV sensitivity of an InGaAs camera. A dual-lens design in which the QDs are incorporated on a removable substrate is demonstrated, which provides UV sensitivity without modification of the InGaAs camera focal plane array. A single-lens design in which the QDs are deposited directly on the focal plane array is demonstrated using both a standard InGaAs focal plane and a substrate-thinned focal plane. Higher UV resolution for the substrate-thinned focal plane is observed.

The use of colloidal quantum dots on focal plane arrays for optical communications

A well-known use of focal plane arrays is to obtain an image of a scene in various photonic bands- most commonly in the visible and infrared domains. Recent developments in colloidal quantum dot technology allow utilization of thin nano-crystals films in conjunction with detector arrays to sensitize detection outside the conventional bands. For example, downshifting PbS quantum dots have been recently demonstrated to produce an image in the UV and VIS bands using SWIR focal planes. In this paper we discuss our results as well as approaches to extend these sensitized focal planes into another mission, UV wireless communications, to achieve a dual-use capability for a conventional imager.