Frank Paqué

Effects of Root Canal Preparation on Apical Geometry Assessed by Micro–Computed Tomography

INTRODUCTION Previous micro-computed tomography analyses of root canal preparation provided data that were usually averaged over canal length. The aim of this study was to compare preparation effects on apical root canal geometry. METHODS Sixty extracted maxillary molars (180 canals) used in prior studies were reevaluated for analyses of the apical 4 mm. Teeth were scanned by using micro-computed tomography before and after canal shaping with FlexMaster, GT-Rotary, Lightspeed, ProFile, ProTaper, instruments or nickel-titanium K-files for hand instrumentation. Apical preparation was to a size #40 in mesiobuccal and distobuccal and #45 in palatal canals except for GT (#20) and ProTaper (#25 in mesiobuccal and distobuccal and #30 in palatal canals, respectively). Data for canal volume changes, the structure model index (quantifying canal cross sections), and untreated surface area were contrasted by using analysis of variance and Scheffé tests. RESULTS Mean mesiobuccal, distobuccal, and palatal canal volumes increased after preparation (P < .05), but differences were noted for preparation techniques. GT rendered the smallest (0.20 +/- 0.14 mm(3)); K-files and ProFile showed the largest volume increases (0.51 +/- 0.20 mm(3) and 0.45 +/- 021 mm(3), P < .05). All canals were slightly rounder in the apical 4 mm after preparation indicated by nonsignificant increases in structure model index. Untreated areas ranged from 4%-100% and were larger in mesiobuccal and palatal canals than in distobuccal ones. Preparation with GT left significantly larger untreated areas in all canal types (P < .05); among root canal types, distobuccal canals had the least amounts of untreated surface areas. CONCLUSIONS Apical canal geometry was affected differently by 6 preparation techniques; preparations with GT instruments to an apical size #20 left more canal surface untouched, which might affect the ability to disinfect root canals in maxillary molars.

Preparation of oval-shaped root canals in mandibular molars using nickel-titanium rotary instruments: a micro-computed tomography study.

INTRODUCTION This study evaluated the prepared surface areas of oval-shaped canals in distal roots of mandibular molars using four different instrumentation techniques. METHODS Teeth were prescanned and reconstructed using micro-computed tomography (MCT) scans at low resolution (68 microm). Forty-eight molars with ribbon-shaped/oval distal root canals were selected and randomly assigned to four groups. Distal canals (n = 12 each) were prepared by circumferential filing using Hedström files to apical size #40 (group H/CF); with ProTaper nickel-titanium rotaries to finishing file 4 (F4) considering the distal canal as 1 canal (group PT/1); ProTaper to F4 considering buccal and oral aspects of the distal canal as 2 individual canals (group PT/2); ProTaper to F4 in a circumferential filing motion (PT/CF). Before and after shaping, teeth were evaluated using MCT at 34-microm resolution. The percentage of prepared surface was assessed for the full canal length and the apical 4 mm. Statistical analysis was performed using analysis of variance and Bonferroni/Dunn multiple comparisons. RESULTS Preoperatively, canal anatomy was statistically similar among the groups (p = 0.56). Mean (+/- standard deviation) untreated areas ranged from 59.6% (+/-14.9, group PT/2) to 79.9% (+/-10.3, PT/1) for the total canal length and 65.2% to 74.7% for the apical canal portion, respectively. Canals in group PT/1 had greater untreated surface areas (p < 0.01) than groups PT/2 and PT/CF. Among all groups, amounts of treated surface areas were statistically similar in the apical 4 mm. CONCLUSIONS Preparations of oval-shaped root canals in mandibular molars left a variable portion of surface area unprepared regardless of the instrumentation technique used. However, considering oval canals as two separate entities during preparation appeared to be beneficial in increasing overall prepared surface.

Hard-Tissue Debris Accumulation Analysis by High-Resolution Computed Tomography Scans

INTRODUCTION Hard-tissue debris accumulation is a potential side effect of root canal instrumentation that has not been systematically investigated. In the current communication, a method to assess this debris using high-resolution microcomputed tomography (microCT) is presented. METHODS Based on prescans, mandibular molars with joining mesial root canals and isthmuses between these were selected (n = 6). The mean volume filled with apparent hard-tissue debris after instrumentation without irrigation was calculated over 2 mm of the mesial canal system by multiplying the voxel volume with the number of voxels representing acquired radiopaque material. Backscattered electron imaging was used to compare the calcium-phosphorus content of this material with that of the root dentin in the same specimen. RESULTS Backscatter scans showed that the accumulated debris viewed in the microCT scans was consistent with root dentin. In the selected canal segments, 29.2% +/- 14.5% of the original canal volume was filled with accumulated debris, which represented a significant change from the preoperative scan (p < 0.01, one-sample t test against zero). Three-dimensional reconstructions of the microCT images visualized the accumulated hard-tissue debris in the whole canal system. CONCLUSIONS The current method appears suitable to quantitatively compare different instrumenting/irrigating regimens on dentin debris accumulation.

Micro-computed tomography evaluation of the preparation of long oval root canals in mandibular molars with the self-adjusting file.

INTRODUCTION The aim of this study was to assess the shaping potential of a novel nickel-titanium instrument, the self-adjusting file (SAF), in long oval root canals in distal roots in mandibular molars. METHODS Twenty mandibular molars with long oval distal root canals were selected and scanned preoperatively and postoperatively by using micro-computed tomography at an original resolution of 20 μm. Canals were shaped with the SAF, three-dimensionally reconstructed, and evaluated for volume, surface area, canal transportation, and prepared surface. Data were statistically contrasted by using paired t tests and regression analysis. RESULTS Preoperatively, canal volume was 7.73 ± 2.13 mm(3), and canal area was 42.83 ± 8.14 mm(2). Volumes and surface areas increased significantly (P < .001) by 4.84 ± 1.73 mm(3) and 3.34 ± 1.73 mm(2), respectively, and no gross preparation errors were detected. Unprepared canal surface varied between individual canals, and mean unprepared surface was 23.5% ± 8.9%. Prepared areas were significantly larger compared with rotary canal preparation done in a previous study. Canal transportation scores were higher in the coronal root canal third (106 ± 50 μm) compared with the apical third (81 ± 49 μm). CONCLUSIONS In vitro, preparation of long oval-shaped root canals in mandibular molars with the SAF was effective and safe. Moreover, shapes generated with the SAF were more complete compared with rotary canal preparation.

The self-adjusting file (SAF). Part 3: removal of debris and smear layer-A scanning electron microscope study.

AIM The aim of this study was to evaluate the cleaning ability of the Self-Adjusting File (SAF) system in terms of removal of debris and smear layer. METHODOLOGY Root canal preparations were performed in 20 root canals using an SAF operated with a continuous irrigation device. The glide path was initially established using a size 20 K-file followed by the SAF file that was operated in the root canal via a vibrating motion for a total of 4 minutes. Sodium hypochlorite (3%) and EDTA (17%) were used as continuous irrigants and were alternated every minute during this initial 4-minute period. This was followed by a 30-second rinse using EDTA applied through a nonactivated SAF and a final flush with sodium hypochlorite. The roots were split longitudinally and subjected to scanning electron microscopy (SEM). The presence of debris and a smear layer in the coronal, middle, and apical thirds of the canal were evaluated through the analysis of the SEM images using five-score evaluation systems based on reference photographs. RESULTS The SAF operation with continuous irrigation, using alternating irrigants, resulted in root canal walls that were free of debris in all thirds of the canal in all (100%) of the samples. In addition, smear layer-free surfaces were observed in 100% and 80% of the coronal and middle thirds of the canal, respectively. In the apical third of the canal, smear layer-free surfaces were found in 65% of the root canals. CONCLUSIONS The operation of the SAF system with continuous irrigation coupled with alternating sodium hypochlorite and EDTA treatment resulted in a clean and mostly smear layer-free dentinal surface in all parts of the root canal.

Root Canal Preparation of Maxillary Molars With the Self-adjusting File: A Micro-computed Tomography Study

INTRODUCTION The aim of this study was to describe the canal shaping properties of a novel nickel-titanium instrument, the self-adjusting file (SAF), in maxillary molars. METHODS Twenty maxillary molars were scanned by using micro-computed tomography at 20-μm resolution. Canals were shaped with the SAF, which was operated with continuous irrigation in a handpiece that provided an in-and-out vibrating movement. Changes in canal volumes, surface areas, and cross-sectional geometry were compared with preoperative values. Canal transportation and the fraction of unprepared canal surface area were also determined. Data were normally distributed and compared by analyses of variance. RESULTS Preoperatively, mean canal volumes were 2.88 ± 1.32, 1.50 ± 0.99, and 4.30 ± 1.89 μm(3) [corrected] for mesiobuccal (MB), distobuccal (DB), and palatal (P) canals, respectively; these values were statistically similar to earlier studies with the same protocol. Volumes and surface areas increased significantly in MB, DB, and P canals; mean canal transportation scores in the apical and middle root canal thirds ranged between 31 and 89 μm. Mean unprepared surfaces were 25.8% ± 12.4%, 22.1% ± 12.0%, and 25.2% ± 11.3% in MB, DB, and P canals, respectively (P > .05) when assessed at high resolution. CONCLUSIONS By using SAF instruments in vitro, canals in maxillary molars were homogenously and circumferentially prepared with little canal transportation.

Root Canal Preparation with a Novel Nickel-Titanium Instrument Evaluated with Micro-computed Tomography: Canal Surface Preparation over Time

INTRODUCTION The aim of this part of an ongoing study was to describe the dentin removal ability of a novel nickel-titanium instrument, the self-adjusting file (SAF), by using micro-computed tomography. METHODS Twenty maxillary incisors were scanned preoperatively at 20-microm resolution and postoperatively after up to 6 minutes of preparation with an SAF with 1.5-mm or 2-mm diameter. SAFs were operated with continuous irrigation in a handpiece that provided an oscillating, in-and-out movement. Changes in canal volume compared with preoperative values as well as unprepared canal surface area were determined. Data were normally distributed and compared by analysis of variance and regression analyses. RESULTS Preoperatively canal volumes were statistically similar in both groups (9.86 +/- 3.97 mm3 and 9.80 +/- 2.67 mm3). Volumes increased during preparation to 13.58 +/- 3.85 mm3 (after 6 minutes with SAF 1.5 mm) and 16.43 +/- 3.64 mm3 (after 5 minutes with SAF 2.0 mm), and overall canal shapes were adequate. Unprepared canal surface area decreased from 63.0% +/- 15.1% (2 minutes with SAF 1.5 mm) to 8.6% +/- 4.1% (5 minutes with SAF 2.0 mm). CONCLUSIONS Preparation of straight root canals in maxillary anterior teeth left little canal surface uninstrumented after shaping with the SAF. The timeframe of clinical application will depend on the amount of desired dentin removal and done with an SAF selected on the basis of apical gauging.

Microtomography-based comparison of reciprocating single-file F2 ProTaper technique versus rotary full sequence.

INTRODUCTION A preparation technique with only 1 single instrument was proposed on the basis of the reciprocating movement of the F2 ProTaper instrument. The present study was designed to quantitatively assess canal preparation outcomes achieved by this technique. METHODS Twenty-five extracted human mandibular first molars with 2 separate mesial root canals were selected. Canals were randomly assigned to 1 of the 2 experimental groups: group 1, rotary conventional preparation by using ProTaper, and group 2, reciprocate instrumentation with 1 single ProTaper F2 instrument. Specimens were scanned initially and after root canal preparation with an isotropic resolution of 20 μm by using a micro-computed tomography system. The following parameters were assessed: changes in dentin volume, percentage of shaped canal walls, and degree of canal transportation. In addition, the time required to reach working length with the F2 instrument was recorded. RESULTS Preoperatively, there were no differences regarding root canal curvature and volume between experimental groups. Overall, instrumentation led to enlarged canal shapes with no evidence of preparation errors. There were no statistical differences between the 2 preparation techniques in the anatomical parameters assessed (P > .01), except for a significantly higher canal transportation caused by the reciprocating file in the coronal canal third. On the other hand, preparation was faster by using the single-file technique (P < .01). CONCLUSIONS Shaping outcomes with the single-file F2 ProTaper technique and conventional ProTaper full-sequence rotary approach were similar. However, the single-file F2 ProTaper technique was markedly faster in reaching working length.

Accumulated hard tissue debris levels in mesial roots of mandibular molars after sequential irrigation steps

AIM To investigate the impact of sequential irrigation procedures on accumulated hard tissue debris (AHTD) levels in canal systems in mesial roots of human mandibular molars after instrumentation. METHODOLOGY Based on pre-scans in a micro-computed tomography (μCT) system, 20 extracted human mandibular molars with joining mesial root canals and an isthmus between these were selected. Canals were instrumented using the ProTaper rotary system until the F3 instrument reached working length. A 1% NaOCl solution was applied during instrumentation, followed by a final 5-mL flush to working length. Subsequently, canals were irrigated with 5 mL of 17% EDTA. Thereafter, passive ultrasonic irrigation (PUI) was performed using 1% NaOCl on three occasions each for 20 s. Percent values of total canal system volume filled with AHTD were calculated based on high-resolution scans after each irrigation step. Data was compared using repeated measure anova followed by paired t-test for individual comparisons. Bonferronis correction was applied for multiple testing; the alpha-type error was set at 1%. RESULTS Instrumentation of the root canals in conjunction with 1% NaOCl irrigation left 6.9 ± 4.2 vol.% of the total canal system volume filled with AHTD. This value was significantly (P < 0.01) reduced to 4.9 ± 3.6 vol.% after EDTA irrigation. Subsequent PUI resulted in a further significant (P < 0.01) reduction to 3.7 ± 2.8 vol.%. CONCLUSIONS In this quantitative study on AHTD reduction, a significant effect of EDTA and PUI was shown. However, approximately half of the debris that accumulated during instrumentation remained in the canal system.

Necrotic pulp tissue dissolution by passive ultrasonic irrigation in simulated accessory canals: impact of canal location and angulation.

AIM To evaluate whether passive ultrasonic irrigation (PUI) of 2.5% NaOCl would dissolve necrotic pulp tissue from simulated accessory root canals (SACs) better than passive placement of the irrigant, when temperature was equilibrated between the two treatments. METHODOLOGY Transparent root canal models (n = 6) were made from epoxy resin. SACs of 0.2 mm diameter were placed at defined angles and positions in the mid-canal and apical area. SACs were filled with necrotic bovine pulp tissue. PUI was performed five times for 1 min each with irrigant replenishment after every minute. Main canal temperature was measured after each minute, and a digital photograph was taken. In control experiments, mock treatments were performed with the same set-up without activation of the file using heated NaOCl to mimic the temperature created by PUI. Experiments were repeated five times. Digital photographs were analysed for the distance of dissolved tissue into the SACs in mm. Overall comparison (sum of dissolved tissue from all five accessory canals) between treatments was performed using paired t-test. Differences between SAC angulation and position after PUI were investigated using anova/Bonferroni (alpha < 0.05). RESULTS Passive ultrasonic irrigation caused a rise in irrigant temperature in the main canal to 53.5 +/- 2.7 degrees C after the fifth minute. PUI dissolved a total of 6.4 +/- 2.1 mm, mock treatment controlled for heat: 1.4 +/- 0.6 mm (P < 0.05). No significant influence of SAC position or angulation was found. CONCLUSIONS Passive ultrasonic irrigation promotes positive tissue-dissolving effects beyond a rise in irrigant temperature.