Frank R. Blood

Incorporation of C14-acetate into intestinal fatty acids of rats with cannulated bile ducts.

Summary 1. Deprivation of bile flow to the rat intestine by means of cannulation of the bile duct did not affect the ability of the intestine to accumulate normal amounts of highly labeled fatty acids after administration of C14-acetate as shown by comparison with sham-operated, pair-fed controls. 2. Total radioactivity in the biliary fatty acids amounted to only 5-16% of the amount found in the intestinal fatty acids in the time periods studied. 3. Fatty acids of combined intestinal and cecal contents of cannulated animals contained more fatty acid but of a lower specific activity than those of control rats.

Chronic toxicity of ethylene glycol in the monkey.

Abstract Incorporation of ethylene glycol in the diet of 2 male monkeys at the level of 0.2% and 1 female monkey at a level of 0.5% for a period of 3 years produced no toxic effects.

Feeding of irradiated chicken, beef, and pineapple jam to dogs

Summary Irradiated and nonirradiated chicken, beef, and pineapple jam were added to the diet of dogs (purebred beagles), in an amount equal to 35% of the solids fed, for a period of two years. No adverse effects were demonstrated as evidenced by growth, hematology, food efficiency, reproduction, mortality, gross and microscopic pathology.

Feeding of irradiated beef to rats

Summary Irradiated and nonirradiated beef were added to the diet of rats in amounts equal to 35% of the solids fed for a period up to two years. A four-generation reproduction study was conducted simultaneously. No adverse effects were demonstrated, as evidenced by growth, hematologic change, food efficiency, reproduction, mortality, or gross and microscopic pathology. It is felt that the somewhat higher incidence of mammary fibroadenomas and adenomas than reported by some other workers is a reflection of age and strain differences rather than diet.

Effect of dietary fat on the disposition of cholesterol-4-C14 in rats.

Summary Rats were maintained for 30 to 76 days on a purified diet containing 20% fat as hydrogenated cottonseed oil, corn oil, or coconut oil and then injected intravenously with a tracer dose of cholesterol-4-C14. In the 6 days following injection higher excretion of C14 was observed in animals maintained on hydrogenated cottonseed oil. Of the activity extracted by petroleum ether after saponification, a lower percentage was present in the digitonin-precipitable fraction in animals fed coconut oil. Smaller amounts of cholesterol were excreted by the coconut oil-fed rats. No consistent differences were observed in total C14 activity of various organs of the different dietary groups at any time period. Neither serum cholesterol nor serum C14 activity was affected by variation of the type of dietary fat given these rats.

Effect of dietary fat on excretion and deposition of C14 from cholesterol-4-C14 in rats.

Summary The influence of a purified diet containing 20% fat as hydrogenated cottonseed oil, corn oil, or coconut oil on fecal excretion and tissue deposition of C14 has been studied in rats injected with cholesterol-4-C14. There was no significant difference between the 3 groups in amount of C14 excreted in feces or deposited in liver and in pooled internal organs (heart, lungs, aorta, and kidneys).

Feeding of irradiated peaches and whole and peeled oranges to monkeys

Summary Inclusion of irradiated peaches, whole oranges, or peeled oranges in the diet of monkeys over a two-year period had no effect on the hematologic picture, growth, microscopic pathology or mortality. Inclusion of orange peel in the diet of monkeys did not increase intraocular pressure.

Effect of Cobalt Administration on Myoglobin Content.

Summary Administration of cobalt to rats at a level sufficient to produce a marked polycythemia did not alter myoglobin concentration.

Effect of x-irradiation on myoglobin content.

To assess the effects of x-irradiation on myoglobin concentration, rats were irradiated with an ld50/30 dose of x-irradiation. Each irradiated rat was paired with a control animal. At approximately weekly intervals for 5 weeks, several pairs were killed and a portion of their skeletal muscle was assayed for myoglobin. No change in the myoglobin concentration was observed although a severe anemia developed in all of the irradiated animals.