Frantisek Bartos

Polyamine levels in normal human serum. Comparison of analytical methods.

Abstract A comparison of levels of the polyamines, putrescine, spermidine and spermine, in individual and pooled normal human serum, as determined by three independent methods, high pressure cation-exchange chromatography (HPCC), radioimmunoassay (RIA) and gas chromatography-mass spectrometry (GC-MS), has been made. Generally good correlations were found for values derived by the three different methods.

Insulin values of normal pancreatic and pancreatic adenomas tissue extracts in children

Abstract Tissues of four pancreatic adenomas of children with clinical hyper-insulinemia and relative hyperinsulinemia, and normal pancreatic tissue were studied. Tissues were extracted and immunoreactive insulin levels measured per gram of dry tissue. Comparisons of findings in two newborn babies with inverted rate glucose/insulin and two children of 11 and 14 yr of age with hyper-insulinemia are reported. Results indicate relative comparable insulin values in adenoma pancreatic tissue in both age groups per gram of tissue, although larger amounts of circulating immunoreactive insulin were found in older children with true hyperinsulinemia. High insulin concentrations were found in one case. These high levels may be related to the severity or type of cell adenoma.

Practical considerations in angiotensin II antibody production

Abstract When cost considerations are paramount, one immunization with angiotensin II-rabbit serum albumin immunogen into multiple sites in rabbits suffices to produce high-titer antisera which are clinically useful for radioimmunoassays. Although sustained antibody responses may occur after one or two immunizations with an angiotensin II-poly- l -lysine immunogen, titers are impractically low for clinical work.