Fred Miya

EFFECT OF PYROGEN ON PHAGOCYTIC DIGESTION AND SURVIVAL OF X-IRRADIATED MICE

Piromen in doses of 1 mu g per injection administered twice daily for 3, 5, or 7 days significantly increased intracellular digestion of chicken erythrocytes by mouse phagocytes. Similar treatment with 0.1 mu g of Piromen for 5 to 7 days also increased intracellular digestion. However, Piromen in doses of 0.1, 1, or 2 mu g per injection administered twice daily for various intervals prior to and after x irradiation failed to increase survival of x- irradiated mice. (auth)

Effect of properdin on whole body irradiated mice and rats.

Summary Under conditions employed postirradiation treatment of mice and rats with partially purified bovine properdin and purified human properdin administered intraperitoneally or intravenously did not afford protection against the effects of whole body x-irradiation. There appeared to be a relationship between delayed mortality and time of injection.

Failure of cell-free spleen extracts to protect irradiated animals.

Summary Postradiation administration of cell-free mouse splenic extract to whole body x-irradiated mice (intraperitoneally) and guinea pigs (intramuscularly) did not result in protection against postradiation death. The treated animals died at an accelerated rate when compared to the controls.

The Properdin System in Mice.

Summary Injection of complement (guinea pig serum) into the laboratory albino mouse failed to alter capacity of these animals to withstand parenterally induced bacterial infection with K. pneumoniae or S. typhimurium. Addition of complement to mouse serum failed to induce bactericidal activity in vitro.

Serologic Analysis of Ehrlich Tumor-Cell Induced Ascites Fluid.∗

Summary Ascites fluid induced in mice by intraperitoneal injection of Ehrlich tumor cells contained specific antibody to S. typhimurium when mice were immunized against this organism. The serum and ascites were identical in their anticomplementary properties and neither contained detectable complement. The serum had normal properdin levels. but the ascites did not contain detectable properdin under the assay conditions employed.

Isolation of partially purified properdin from bovine serum by cold ethanol fractionation.

Summary A procedure for isolation of properdin from bovine serum using cold ethanol fractionation technics is reported. Results indicate that high yields of properdin per unit volume are obtainable without use of zymosan.