Friedhelm Maywald
Bayer
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Featured researches published by Friedhelm Maywald.
Gene | 1988
Friedhelm Maywald; Thomas Böldicke; Gerhard Gross; Ronald Frank; Helmut Blöcker; Andreas Meyerhans; Konrad Schwellnus; Jürgen Dr. Ebbers; Wolfgang Bruns; Gerd Reinhardt; Eugen Schnabel; Werner Schröder; Hans Fritz; John H. Collins
As a basis for a protein design project, we decided to produce the human pancreatic secretory trypsin inhibitor (PSTI) in its active form. Total gene synthesis was carried out efficiently by (i) computer design of the gene fragments, (ii) synthesis of the oligodeoxynucleotides by the segmental support method, and (iii) assembly of double strands under optimized ligation conditions. Fusion to the ompA gene signal peptide led to secretion of processed PSTI in various constructions, with or without additional amino acids (aa) at the N-terminus. The secreted proteins (56 to 63 aa) were biologically active, suggesting that the three cysteine bridges were correctly formed. Surprisingly, after induction the product was found almost exclusively in the culture medium. Variants of PSTI with Asp or Asn at aa positions 21 and 29 [sequences published by Greene et al., Methods Enzymol. (1976) 813-825, and by Yamamoto et al., Biochem. Biophys. Res. Commun. (1985) 605-612] showed the same Ki for both human and porcine trypsin.
Biological chemistry Hoppe-Seyler | 1990
John H. Collins; Michael Szardenings; Friedhelm Maywald; Helmut Blöcker; Ronald Frank; Hans-Jürgen Hecht; Birger Vasel; Dietmar Schomburg; Edwin Fink; Hans Fritz
FEBS Journal | 1988
Thomas Böldicke; Silke Kindt; Friedhelm Maywald; Gabriele Fitzlaff; Michael Böcher; Ronald Frank; John Collins
Archive | 1991
John Prof. Dr. Collins; Helmut Dr. Blöcker; Ronald Dr. Frank; Friedhelm Maywald; Hans Prof Dr Fritz; Wolfgang Bruns
Archive | 1987
John Prof. Dr. Collins; Helmut Dr. Blöcker; Ronald Dr. Frank; Friedhelm Maywald; Hans Prof Dr Fritz; Wolfgang Bruns
Archive | 1988
Thomas Böldicke; Silke Kindt; Friedhelm Maywald; Gaby Fitzlaff; Ronald Frank; John H. Collins
Archive | 1987
Rolf-Dieter Prof. Dr. Hesch; John Prof. Dr. Collins; Ronald Frank; Friedhelm Maywald; Friedrich Dr. Götz; Roland Dr. Dipl.-Biol. Netzker; Konradt Dr. Dipl.-Chem. Schwellnus; Andreas Meyerhans; Helmut Blöcker
Archive | 1988
Collins John; John H. Collins; Bloecker Helmut; Helmut Bloecker; Frank Ronald; Ronald Frank; Maywald Friedhelm; Friedhelm Maywald; Fritz Hans; Hans Fritz; Bruns Wolfgang; Wolfgang Bruns
Archive | 1988
Wolfgang Bruns; Ronald Frank; Helmut Bloecker; John H. Collins; Friedhelm Maywald; Hans Fritz
Archive | 1988
John H. Collins; Helmut Bloecker; Ronald Frank; Friedhelm Maywald; Hans Fritz; Wolfgang Bruns