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Dive into the research topics where Fujio Yu is active.

Publication


Featured researches published by Fujio Yu.


Microbiology | 1992

Development of a host-vector system in a Rhodococcus strain and its use for expression of the cloned nitrile hydratase gene cluster.

Yoshihiro Hashimoto; Makoto Nishiyama; Fujio Yu; Ichiro Watanabe; Sueharu Horinouchi; Teruhiko Beppu

Two different types of plasmid were isolated from strains of Rhodococcus rhodochrous. Two plasmids, of the same type but from different strains, were combined with Escherichia coli plasmids carrying antibiotic resistance markers to develop E. coli-Rhodococcus shuttle vectors. The ampicillin and kanamycin resistance markers served for selection in Rhodococcus. Electroporation was used to introduce recombinant plasmid DNA into R. rhodochrous ATCC 12674 at a frequency of 5 x 10(7) transformants per microgram DNA. With these host-vector and transformation systems, the nitrile hydratase and amidase genes of a Rhodococcus strain were introduced into the host strain and were efficiently expressed.


Archive | 1990

Process for the production of 4-halo-3-hydroxybutyronitrile with Corynebacterium or Microbacterium

Tetsuji Nakamura; Fujio Yu; Ichiro Watanabe


Archive | 1992

Process for the production of R(-)-4-halo-3-hydroxybutyronitrile

Tetsuji Nakamura; Fujio Yu


Archive | 1991

Circular plasmids derived from the genus rhodococcus

Fujio Yu; Yoshihiro Hashimoto


Archive | 1994

Hybrid plasmid vectors, recombinant plasmids containing genes encoding nitrile degrading enzymes, transformants containing the recombinant plasmids and methods of producing amides and acids using the transformants

Teruhiko Beppu; Sueharu Horinouchi; Makoto Nishiyama; Fujio Yu; Yoshihiro Hashimoto


Archive | 1991

Novel circular plasmids derived from the genus rhodococcus

Fujio Yu; Yoshihiro Hashimoto


Archive | 2011

Fluorescent substrate for detection of enzymatic activity of nitrile-related enzyme

Yasuteru Urano; Tetsuo Nagano; Tomoe Ohta; Fujio Yu


Archive | 2015

Procédé de production d'un ester d'acide méthacrylique et nouvelle synthase d'ester d'acide méthacrylique

Yasuhisa Asano; Eiji Sato; Fujio Yu; Wataru Mizunashi


Archive | 2008

Verbesserte halohydrin-epoxidase

Takanori Akiyama; Fujio Yu; Fumiaki Watanabe; Yuya Takikawa; Eiji Sato


Archive | 2008

Halohydrine époxidase améliorée

Takanori Akiyama; Fujio Yu; Fumiaki Watanabe; Yuya Takikawa; Eiji Sato

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