Fujio Yu
University of Tokyo
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Publication
Featured researches published by Fujio Yu.
Microbiology | 1992
Yoshihiro Hashimoto; Makoto Nishiyama; Fujio Yu; Ichiro Watanabe; Sueharu Horinouchi; Teruhiko Beppu
Two different types of plasmid were isolated from strains of Rhodococcus rhodochrous. Two plasmids, of the same type but from different strains, were combined with Escherichia coli plasmids carrying antibiotic resistance markers to develop E. coli-Rhodococcus shuttle vectors. The ampicillin and kanamycin resistance markers served for selection in Rhodococcus. Electroporation was used to introduce recombinant plasmid DNA into R. rhodochrous ATCC 12674 at a frequency of 5 x 10(7) transformants per microgram DNA. With these host-vector and transformation systems, the nitrile hydratase and amidase genes of a Rhodococcus strain were introduced into the host strain and were efficiently expressed.
Archive | 1990
Tetsuji Nakamura; Fujio Yu; Ichiro Watanabe
Archive | 1992
Tetsuji Nakamura; Fujio Yu
Archive | 1991
Fujio Yu; Yoshihiro Hashimoto
Archive | 1994
Teruhiko Beppu; Sueharu Horinouchi; Makoto Nishiyama; Fujio Yu; Yoshihiro Hashimoto
Archive | 1991
Fujio Yu; Yoshihiro Hashimoto
Archive | 2011
Yasuteru Urano; Tetsuo Nagano; Tomoe Ohta; Fujio Yu
Archive | 2015
Yasuhisa Asano; Eiji Sato; Fujio Yu; Wataru Mizunashi
Archive | 2008
Takanori Akiyama; Fujio Yu; Fumiaki Watanabe; Yuya Takikawa; Eiji Sato
Archive | 2008
Takanori Akiyama; Fujio Yu; Fumiaki Watanabe; Yuya Takikawa; Eiji Sato