Fumikazu Mizukoshi

Localization of triphosphoinositide in the cochlea

SummaryTriphosphoinositide (TPI) has been demonstrated to be a receptor for aminoglycosides in the cochlea and may regulate ionic permeability by its binding with Ca++. This phospholipid was localized by a protein A-gold technique in the cochlea at the electronmicroscopic level. TPI was prepared by a neomycin column and antibodies to it were raised in rabbits. The antibody used in this study reacted virtually only to TPI among the tested lipids. TPI was localized mainly at stereocilia, cuticular plates, head plates of Deiters cells, plasma membrane, and mitochondria of various cells in the organ of Corti. In the vascular stria, TPI was found mainly at the plasma membrane of basal infoldings of the marginal cells. Possible physiological and pathophysiological roles of TPI in the cochlea are briefly discussed.

Immunocytochemical detection of triphosphoinositide in vestibular hair cells

SummaryTriphosphoinositide (TPI), an aminoglycoside receptor and a possible regulator of cationic permeation through its ability to bind with Ca++, was localized by the protein-A gold technique in vestibular sensory epithelia using an antibody highly specific to TPI. TPI was detected on the stereocilia, kinocilia, and cuticular plate of hair cells, and in the reticular membrane of supporting cells. The cilia of hair cells are damaged by aminoglycosides at a relatively early stage of toxicity. Ca++-regulated bioactivity in this area is probably involved.

Distribution of Goblet Cells in the Rat Nasal Septum by Use of Quantitative Histological Technique

In this quantitative, histological study, both goblet cell number and distribution of secretory granules in normal septal mucosa of rats were examined by computer-based color image analysis combined with a new, simple whole-mount method. Our new method has proved to be a very useful and highly accurate means of examination. The count and size of the mucous area of goblet cells decreased toward the antero-posterior direction with the exception of the apical region of the septum. The regional difference characteristic of the goblet cell is noteworthy. The results are assumed to be closely related to both flow direction of mucous blanket and distribution of nasal gland orifices. Goblet cells are assumed to be controlled by some regulatory factor of the secretory system and to play an important role in nasal physiological mechanism.

Early Seasonal Treatment of Japanese Cedar Pollinosis with Topical Glucocorticosteroids.

The effects of early seasonal administration of topical glucocorticosteroids for Japanese cedar pollinosis were examined. In a group that received the drug one week prior to the onset of the season, nasal symptoms were significantly suppressed in the early period of the season. When treatment was started at the onset of the season, the same effects were achieved but commenced one week later. In both groups, nasal congestion was greatly alleviated.

Longitudinal Median Sternotomy for Extirpation of Cervical Tumors Extended to the Upper Mediastinum

The cervical tumor observed in five cases extending to the upper mediastinum was extirpated in our department by using a combination of a neck dissection and a longitudinal median sternotomy.These tumors were originated from thyroid,thymus,and cervical nerve. The longitudinal median sternotomy is a useful approach to the upper mediastinum in order to keep a good operation view,to decrease bleeding volume,and to shorten operation time. Therefore,this approach should be adapted more for the extirpation of cervical tumor which extends to the upper mediastinum and for a mediastinal dissection.

Case report and survey of patients treated adenocarcinoma of the ethmoid sinus in our university since 1964.

This is a case report of a 68-year-old male with tumor of the ethmoid sinus diagnosed histologically as adenocarcinoma. The patient had complained of nasal bleeding and was treated with surgery, followed by irradiation, chemotherapy and intraarterial infusion of 5FU. The most important part of the treatment was considered to be surgery. The patient is alive 7 months after the initial treatment without any evidence of recurrence of the tumor.From 1964 to 1984, 182 patients with carcinoma of the nasal cavity and paranasal sinuses were treated in our university. In 9 cases (4.9%), the primary site was an ethmoid sinus. The histological diagnoses were squamous cell carcinoma (5), undifferentiated carcinoma (3) and adenocarcinoma (1). The 5-year survival rate was 50%, but in cases of undifferentiated carcinoma, or when the eyes were involved, the prognosis was very poor, none of these patients survived for 5 years.