Fumitake Nishimura

A simple biofilm model of bacterial competition for attached surface.

A simple biofilm model of competition in bacterial growth for an attached surface is developed. Competition for the attached surface is expressed with the crowded and detachment effects. The developed model is verified by comparing simulated results with data obtained in the experiments of batch culture of nitrifier and continuous treatment of actual sewage with biofilm reactor. This model can favorably simulate the growth competition between autotrophic and heterotrophic bacteria for the attached surface. Then some parameters for nitrification process are discussed with this model. It is clarified that the effective removal of organic matter before nitrification tank is required for effective nitrification in the biofilm reactor.

O3/H2O2 Process for Both Removal of Odorous Algal-Derived Compounds and Control of Bromate Ion Formation

The limitation of ozonation and the applicability of ozone/hydrogen peroxide process with a source water for a water supply using a flow-through type contactor were discussed. The water sample was pre-treated in a lab and spiked with bromide ion in the concentration range from 39 to 515 μg/L, and both 2-MIB and geosmin, odorous algal-derived compounds, from 58 to 609 ng/L under the hydrogen peroxide dose of 0 to 3.7 mg/L. When the initial concentration of Br− was around 50 μg/L, the formation of BrO3 − was controlled at less than 10 μg/L at the ozone dose of 2 mg/L, however the concentration of 2-MIB was over 10 ng/L in some cases with its initial concentration of around 100 ng/L during ozonation. When the initial concentration of Br− exceeds 100 μg/L, it seems very difficult to meet the standard for drinking water quality even with the initial 2-MIB concentration of 100 ng/L. The dose of H2O2 from 0.5 to 2.3 mg/L attained the standard for drinking water quality with the initial concentrations of Br− of approximately 50 μg/L and of 2-MIB of up to 500 ng/L at the ozone dose of 2 mg/L. When the initial concentration of 2-MIB was around 100 ng/L with the initial Br− concentration of up to 200 μg/L, the H2O2 dose of 1.7 mg/L also attained the standard for drinking water quality. When the initial concentration of Br− was around 500 μg/L, although the higher dose of H2O2 was required for the control of BrO3 − formation and the increase of the initial concentration of 2-MIB requires more H2O2 dose, the higher dose of H2O2 could attain the standard for drinking water quality of BrO3 − and 2-MIB (geosmin) simultaneously.

Behavior of inorganic elements during sludge ozonation and their effects on sludge solubilization.

The behavior of inorganic elements (including phosphorus, nitrogen, and metals) during sludge ozonation was investigated using batch tests and the effects of metals on sludge solubilization were elucidated. A decrease of ∼ 50% in the ratio of sludge solubilization was found to relate to a high iron content 80-120 mgFe/gSS than that of 4.7-7.4 mgFe/gSS. During sludge ozonation, the pH decreased from 7 to 5, which resulted in the dissolution of chemically precipitated metals and phosphorus. Based on experimental results and thermodynamic calculation, phosphate precipitated by iron and aluminum was more difficult to release while that by calcium released with decrease in pH. The release of barium, manganese, and chrome did not exceed 10% and was much lower than COD solubilization; however, that of nickel, copper, and zinc was similar to COD solubilization. The ratio of nitrogen solubilization was 1.2 times higher than that of COD solubilization (R(2)=0.85). Of the total nitrogen solubilized, 80% was organic nitrogen. Because of their high accumulation potential and negative effect on sludge solubilization, high levels of iron and aluminum in both sewage and sludge should be considered carefully for the application of the advanced sewage treatment process with sludge ozonation and phosphorus crystallization.

Removal of high concentration ammonia from wastewater by a combination of partial nitrification and anammox treatment

Attached growth reactors were developed separately for solids retention time (SRT)-controlled partial nitrification and for anaerobic ammonia oxidation (Anammox) treatment, and a new nitrogen removal process is proposed for wastewater containing highly concentrated ammonia. For partial nitrification, an attached growth medium of polyurethane foam was used. Partial nitrification was achieved stably under a SRT of 4 days, and the abundance ratio of NO -N to the sum of NH -N, NO -N and NO -N was approximately 0.8 after 10 days. Under a SRT of 4 days, the amoA gene concentrations of ammonia-oxidizing bacteria increased from 1×108 to 7×108 copies/l, whereas the 16S ribosomal RNA (16S rRNA) gene concentrations of nitrite-oxidizing bacteria did not increase. These results indicate that SRT-controlled operation is a promising technology for achieving partial nitrification. For the Anammox treatment, an attached growth medium of non-woven fabric was used. Inorganic nitrogen removal of approximately 80–90% was observed at an inorganic nitrogen loading rate of over 10 kgN/(m3-medium·d) and an influent nitrogen concentration of 400 mgN/l. Our non-woven fabric reactor showed similar or superior Anammox performance to that reported previously. By using a combination of these two rectors, we can develop a method that combines partial nitrification and Anammox treatment for effective and stable nitrogen removal.

Effects of microbial activity on perfluorinated carboxylic acids (PFCAs) generation during aerobic biotransformation of fluorotelomer alcohols in activated sludge

Biotransformation of fluorotelomer alcohols (FTOHs) in wastewater treatment plants (WWTPs) can release toxic intermediates and perfluorinated carboxylic acids (PFCAs) to the aqueous environment. However, little information is known about the role of relevant microbial activity (i.e., autotrophs and/or heterotrophs) in biotransformation of FTOHs. Additionally, the dynamics of microbial community in sludge after exposure to FTOHs remain unclear. In the present research, using domestic and industrial WWTP sludge, we performed lab-scale batch experiments to characterize the FTOHs biodegradation property under aerobic condition. Both heterotrophs and the autotrophs were associated with FTOHs biotransformation. However, the microbial activity influenced PFCAs generation efficiency. Autotrophs based on ammonia oxidation (50mgN/L) resulted in more effective generation of PFCAs than heterotrophs based on glucose (200mgC/L) metabolism. Moreover, autotrophs generated more amounts of short-chain PFCAs (carbon number ≤7) than the heterotrophs. The ammonia monooxygenase (AMO) in ammonia oxidizing microorganisms (AOMs) are suggested as responsible for the enhanced generation of PFCAs during FTOHs biotransformation. In the sludge that had been exposed to poly- and perfluorinated alkyl substances in an industrial WWTP, Chlorobi was the predominant microorganisms (36.9%), followed by Proteobacteria (20.2%), Bacteroidetes (11.1%), Chloroflexi (6.2%), Crenarchaeota (5.6%), Planctomycetes (4.2%), and Acidobacteria (3.5%). In the present research, the dosed 8:2 FTOH (12.1mg/L) and its biotransformation products (intermediates and PFCAs) could force a shift in microbial community composition in the sludge. After 192h, Proteobacteria significantly increased and dominated. These results provide knowledge for comprehending the effects of microbial activity on FTOHs biodegradation and the information about interaction between microbial community and the exposure to FTOHs in activated sludge.

Applicability of Corbicula as a bioindicator for monitoring organochlorine pesticides in fresh and brackish waters

The applicability of Corbicula as a bioindicator for monitoring organochlorine pesticides (OCPs) in fresh and brackish waters is presented here. Differences in isomer compositions and OCP bioaccumulation levels were analyzed in western Japan and the Pearl River Delta (PRD) in China. Isomer compositions of DDTs, chlordanes, and HCHs were significantly different between the two areas because of their different historical uses and property of the chemicals. This is represented by the (DDE + DDD)/DDT ratio in Corbicula, ranging 4.9–39 in western Japan and 1.1–2.4 in the PRD. However, isomer compositions in Corbicula reflected those in water, and the different patterns in Corbicula likely reflected the usage history. Concentrations of dissolved oxygen, suspended solids, and volatile suspended solids in water, and the difference in species did not influence OCP bioaccumulative levels in Corbicula when conducting biomonitoring. These levels are likely similar to those in Mytilus galloprovincialis. Therefore, Corbicula could be an appropriate bioindicator for monitoring OCPs in fresh and brackish waters.

Investigation of 1,4-dioxane originating from incineration residues produced by incineration of municipal solid waste

As a groundwater contaminant, 1,4-dioxane is of considerable concern because of its toxicity, refractory nature to degradation, and rapid migration within an aquifer. Although landfill leachate has been reported to contain significant levels of 1,4-dioxane, the origin of 1,4-dioxane in leachate has not been clarified until now. In this study, the origins of 1,4-dioxane in landfill leachate were investigated at 38 landfill sites and three incineration plants in Japan. Extremely high levels of 1,4-dioxane 89 and 340 microg l(-1), were detected in leachate from two of the landfill sites sampled. Assessments of leachate and measurement of 1,4-dioxane in incineration residues revealed the most likely source of 1,4-dioxane in the leachate to be the fly ash produced by municipal solid waste incinerators. Effective removal of 1,4-dioxane in leachate from fly ash was achieved using heating dechlorination systems. Rapid leaching of 1,4-dioxane observed from fly ash in a sequential batch extraction indicated that the incorporation of a waste washing process could also be effective for the removal of 1,4-dioxane in fly ash.

Bioaccumulation and primary risk assessment of persistent organic pollutants with various bivalves

Field surveys on persistent organic pollutant (POP) bioaccumulation were conducted with oysters, clams and scallops whose consumption amount accounted for large shares in the total consumption of shellfish in Japan. There was no numerical difference in bioaccumulation characteristics between oysters, clams, scallops, Corbicula and Mytilus galloprovincialis. Therefore, it was clear that the bioaccumulation characteristics in oysters, clams and scallops, which are important for food, could be ascertained by using the monitoring results with Corbicula and M. galloprovincialis which are easily sampled in various water areas in the world. Non-cancer risk (hazard quotient, HQ) and cancer risk (excess cancer risk, ΔR) via shellfish ranged from 10⁻⁸ to 10⁻⁴ and from 10⁻¹¹ to 10⁻⁷, respectively, at sampling points, which showed the risks of POP exposure via shellfish to be low enough. However, concerning the intake of other food, the importance of dieldrin monitoring should be suggested in Japan. Based on these results, the effectiveness of primary risk assessment could be suggested for screening chemicals whose preferential monitoring is needed.

Effect of alkaline microwaving pretreatment on anaerobic digestion and biogas production of swine manure

Microwave assisted with alkaline (MW-A) condition was applied in the pretreatment of swine manure, and the effect of the pretreatment on anaerobic treatment and biogas production was evaluated in this study. The two main microwaving (MW) parameters, microwaving power and reaction time, were optimized for the pretreatment. Response surface methodology (RSM) was used to investigate the effect of alkaline microwaving process for manure pretreatment at various values of pH and energy input. Results showed that the manure disintegration degree was maximized of 63.91% at energy input of 54 J/g and pH of 12.0, and variance analysis indicated that pH value played a more important role in the pretreatment than in energy input. Anaerobic digestion results demonstrated that MW-A pretreatment not only significantly increased cumulative biogas production, but also shortened the duration for a stable biogas production rate. Therefore, the alkaline microwaving pretreatment could become an alternative process for effective treatment of swine manure.

Nitrogen behavior during sludge ozonation: a long-term observation by pilot experiments

Sludge ozonation is a promising technology for dealing with the increasing challenge of excess sludge treatment and disposal. However, nitrogen behavior during sludge ozonation and subsequent biological removal remains unclear. To clarify the feasibility and stability of oxidizing organic nitrogen (released during sludge ozonation) in the bioreactor (but not during ozonation), and the best operational conditions for sludge ozonation, nitrogen behavior was investigated by a long-term observation. The results showed that when inlet ozone concentration increased from 30 to 80 mg O3/L, and ozonation time decreased from 29 to 11 h, less soluble organic nitrogen was oxidized to ammonia (from 66.1 to 18.7% of soluble total nitrogen). This can reduce the operational costs of sludge ozonation. Furthermore, it is feasible to convert organic nitrogen to nitrate by biological processes because full nitrification was restored in three weeks after shock loading of organic nitrogen owing to the change in ozonation conditions. After combining sludge ozonation with the anaerobic/oxic process, the mass balance for nitrogen showed that nitrogen in the excess sludge decreased with increasing sludge reduction rate. The decreased nitrogen in the excess sludge was mainly transformed to nitrogen gas by denitrification, whereas nitrogen in the effluent did not increase noticeably.