G.A. van Koningsveld
Wageningen University and Research Centre
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Publication
Featured researches published by G.A. van Koningsveld.
FEBS Letters | 2004
L.K. Ozimek; S.A F T van Hijum; G.A. van Koningsveld; van der Marc Maarel; G.H. van Geel-Schutten; Lubbert Dijkhuizen
Bacterial fructosyltransferases (FTFs) are retaining‐type glycosidases that belong to family 68 of glycoside hydrolases. Recently, the high‐resolution 3D structure of the Bacillus subtilis levansucrase has been solved [Meng, G. and Futterer, K., Nat. Struct. Biol. 10 (2003) 935–941]. Based on this structure, the catalytic nucleophile, general acid/base catalyst, and transition state stabilizer were identified. However, a detailed characterization of site‐directed mutants of the catalytic nucleophile has not been presented for any FTF enzyme. We have constructed site‐directed mutants of the three putative catalytic residues of the Lactobacillus reuteri 121 levansucrase and inulosucrase and characterized the mutant proteins. Changing the putative catalytic nucleophiles D272 (inulosucrase) and D249 (levansucrase) into their amido counterparts resulted in a 1.5–4×105 times reduction of total sucrase activity.
Journal of Agricultural and Food Chemistry | 2003
S.V.E. Prigent; Harry Gruppen; Antonie J. W. G. Visser; G.A. van Koningsveld; G.A.H. de Jong; A.G.J. Voragen
Journal of Agricultural and Food Chemistry | 2001
L.A.M. Pouvreau; Harry Gruppen; S.R. Piersma; L.A.M. van den Broek; G.A. van Koningsveld; A.G.J. Voragen
Journal of Agricultural and Food Chemistry | 2002
S. Gonzales-Perez; K.B. Merck; J.M. Vereijken; G.A. van Koningsveld; Harry Gruppen; A.G.J. Voragen
Trends in Food Science and Technology | 2004
Lynn Heng; G.A. van Koningsveld; Harry Gruppen; M.A.J.S. van Boekel; Jean-Paul Vincken; Jacques P. Roozen; A.G.J. Voragen
Journal of Agricultural and Food Chemistry | 2001
G.A. van Koningsveld; Harry Gruppen; H.H.J. de Jongh; G. Wijngaards; M.A.J.S. van Boekel; P. Walstra; A.G.J. Voragen
Journal of Agricultural and Food Chemistry | 2005
Bas J. H. Kuipers; G.A. van Koningsveld; Arno C. Alting; F. Driehuis; Harry Gruppen; A.G.J. Voragen
Journal of Agricultural and Food Chemistry | 2003
L.A.M. Pouvreau; Harry Gruppen; G.A. van Koningsveld; L.A.M. van den Broek; A.G.J. Voragen
Journal of Agricultural and Food Chemistry | 2002
Stef J. Koppelman; G.A. van Koningsveld; A.C. Knulst; Harry Gruppen; I.G.A.J. Pigmans; H.H.J. de Jongh
Journal of Agricultural and Food Chemistry | 2002
G.A. van Koningsveld; P. Walstra; Harry Gruppen; G. Wijngaards; M.A.J.S. van Boekel; A.G.J. Voragen
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