G. E. Morfill

Plasma medicine: an introductory review

This introductory review on plasma health care is intended to provide the interested reader with a summary of the current status of this emerging field, its scope, and its broad interdisciplinary approach, ranging from plasma physics, chemistry and technology, to microbiology, biochemistry, biophysics, medicine and hygiene. Apart from the basic plasma processes and the restrictions and requirements set by international health standards, the review focuses on plasma interaction with prokaryotic cells (bacteria), eukaryotic cells (mammalian cells), cell membranes, DNA etc. In so doing, some of the unfamiliar terminology—an unavoidable by-product of interdisciplinary research—is covered and explained. Plasma health care may provide a fast and efficient new path for effective hospital (and other public buildings) hygiene— helping to prevent and contain diseases that are continuously gaining ground as resistance of pathogens to antibiotics grows. The delivery of medically active substances at the molecular or ionic level is another exciting topic of research through effects on cell walls (permeabilization), cell excitation (paracrine action) and the introduction of reactive species into cell cytoplasm. Electric fields, charging of surfaces, current flows etc can also affect tissue in a controlled way. The field is young and hopes are high. It is fitting to cover the beginnings in New Journal of Physics, since it is the physics (and non- equilibrium chemistry) of room temperature atmospheric pressure plasmas that have made this development of plasma health care possible.

Focus on Plasma Medicine

Plasma Healthcare is an emerging interdisciplinary research topic of rapidly growing importance, exploring considerable opportunities at the interface of plasma physics, chemistry and engineering with life sciences. Some of the scientific discoveries reported so far have already demonstrated clear benefits for healthcare in areas of medicine, food safety, environmental hygiene, and cosmetics. Examples include ongoing studies of prion inactivation, chronic wound treatment and plasma-mediated cancer therapy. Current research ranges from basic physical processes, plasma chemical design, to the interaction of plasmas with (i) eukaryotic (mammalian) cells; (ii) prokaryotic (bacteria) cells, viruses, spores and fungi; (iii) DNA, lipids, proteins and cell membranes; and (iv)xa0living human, animal and plant tissues in the presence of biofluids. Of diverse interests in this new field is the need for hospital disinfection, in particular with respect to the alarming increase in bacterial resistance to antibiotics, the concomitant needs in private practices, nursing homes etc, the applications in personal hygiene—and the enticing possibility to design plasmas as possible pharmaceutical products, employing ionic as well as molecular agents for medical treatment. The delivery of the reactive plasma agents occurs at the gaseous level, which means that there is no need for a carrier medium and access to the treatment surface is optimal. This focus issue provides a close look at the current state of the art in Plasma Medicine with a number of forefront research articles as well as an introductory review. Focus on Plasma Medicine Contents Application of epifluorescence scanning for monitoring the efficacy of protein removal by RF gas-plasma decontamination Helen C Baxter, Patricia R Richardson, Gaynor A Campbell, Valeri I Kovalev, Robert Maier, James S Barton, Anita C Jones, Greg DeLarge, Mark Casey and Robert L Baxter Inactivation factors of spore-forming bacteria using low-pressure microwave plasmas in an N2 and O2 gas mixture M K Singh, A Ogino and M Nagatsu Degradation of adhesion molecules of G361 melanoma cells by a non-thermal atmospheric pressure microplasma H J Lee, C H Shon, Y S Kim, S Kim, G C Kim and M G Kong The acidification of lipid film surfaces by non-thermal DBD at atmospheric pressure in air A Helmke, D Hoffmeister, N Mertens, S Emmert, J Schuette and W Vioel Reduction and degradation of amyloid aggregates by a pulsed radio-frequency cold atmospheric plasma jet D L Bayliss, J L Walsh, G Shama, F Iza and M G Kong The effect of low-temperature plasma on bacteria as observed by repeated AFM imaging Rene Pompl, Ferdinand Jamitzky, Tetsuji Shimizu, Bernd Steffes, Wolfram Bunk, Hans-Ulrich Schmidt, Matthias Georgi, Katrin Ramrath, Wilhelm Stolz, Robert W Stark, Takuya Urayama, Shuitsu Fujii and Gregor Eugen Morfill Removal and sterilization of biofilms and planktonic bacteria by microwave-induced argon plasma at atmospheric pressure Mi Hee Lee, Bong Joo Park, Soo Chang Jin, Dohyun Kim, Inho Han, Jungsung Kim, Soon O Hyun, Kie-Hyung Chung and Jong-Chul Park Cell permeabilization using a non-thermal plasma M Leduc, D Guay, R L Leask and S Coulombe Physical and biological mechanisms of direct plasma interaction with living tissue Danil Dobrynin, Gregory Fridman, Gary Friedman and Alexander Fridman Nosocomial infections-a new approach towards preventive medicine using plasmas G E Morfill, T Shimizu, B Steffes and H-U Schmidt Generation and transport mechanisms of chemical species by a post-discharge flow for inactivation of bacteria Takehiko Sato, Shiroh Ochiai and Takuya Urayama Low pressure plasma discharges for the sterilization and decontamination of surfaces F Rossi, O Kylian, H Rauscher, M Hasiwa and D Gilliland Contribution of a portable air plasma torch to rapid blood coagulation as a method of preventing bleeding S P Kuo, O Tarasenko, J Chang, S Popovic, C Y Chen, H W Fan, A Scott, M Lahiani, P Alusta, J D Drake and M Nikolic A two-dimensional cold atmospheric plasma jet array for uniform treatment of large-area surfaces for plasma medicine QY Nie, Z Cao, C S Ren, D Z Wang and M G Kong A novel plasma source for sterilization of living tissues E Martines, M Zuin, R Cavazzana, E Gazza, G Serianni, S Spagnolo, M Spolaore, A Leonardi, V Deligianni, P Brun, M Aragona, I Castagliuolo and P Brun Designing plasmas for chronic wound disinfection T Nosenko, T Shimizu and G E Morfill Plasma medicine: an introductory review M G Kong, G Kroesen, G Morfill, T Nosenko, T Shimizu, J van Dijk and J L Zimmermann

Nosocomial infections—a new approach towards preventive medicine using plasmas

A new, very efficient, large area scalable and robust electrode design for plasma production in air at atmosphere pressures has been developed and tested. This has made the development of a plasma dispenser for hospital disinfection possible, which has certain advantages over current fluid disinfection systems. The properties of this device are presented, in particular the bactericidal and fungicidal efficiency, and the advantages are described. Such plasma dispensers could play an important role in the future fight against the alarming and growing threat posed by nosocomial (=hospital and community associated) bacterial infections.

Designing plasmas for chronic wound disinfection

Irradiation with low-temperature atmospheric-pressure plasma provides a promising method for chronic wound disinfection. To be efficient for this purpose, plasma should meet the following criteria: it should significantly reduce bacterial density in the wounded area, cause a long-term post-irradiation inhibition of bacterial growth, yet without causing any negative effect on human cells. In order to design plasmas that would satisfy these requirements, we assessed the relative contribution of different components with respect to bactericidal properties due to irradiation with argon plasma. We demonstrate that plasma-generated UV radiation is the main short-term sterilizing factor of argon plasma. On the other hand, plasma-generated reactive nitrogen species (RNS) and reactive oxygen species (ROS) cause a long-term after-irradiation inhibition of bacterial growth and, therefore, are important for preventing wound recolonization with bacteria between two treatments. We also demonstrate that at certain concentrations plasma-generated RNS and ROS cause significant reduction of bacterial density, but have no adverse effect on human skin cells. Possible mechanisms of the different effects of plasma-generated reactive species on bacteria and human cells are discussed. The results of this study suggest that argon plasma for therapeutic purposes should be optimized in the direction of reducing the intensity of plasma-generated UV radiation and increasing the density of non-UV plasma products.

Effects of cold atmospheric plasmas on adenoviruses in solution

Experiments were performed with cold atmospheric plasma (CAP) to inactivate adenovirus, a non-enveloped double stranded DNA virus, in solution. The plasma source used was a surface micro-discharge technology operating in air. Various plasma diagnostic measurements and tests were performed in order to determine the efficacy of CAPs and to understand the inactivation mechanism(s). Different stages of the adenovirus ‘life cycle’ were investigated—infectivity and gene expression as well as viral replication and spread. Within 240xa0s of CAP treatment, inactivation of up to 6 decimal log levels can be achieved.

On the role of dust in the summer mesopause

Abstract We propose that dust formed at the cool summer mesopause may have optical properties very different from that measured for bulk material of ice. The smallness of the dust and possible surface impurities may lead to high photoelectric yields and low workfunctions. For such reasons the dust in the summer mesopause may, at least occasionally, be charged to substantial positive surface potentials while pure ice, with its high photoelectric workfunction, would be charged to low and negative potentials by collisions with plasma particles. The presence of ‘dressed’ dust particles, with surface potentials of some volts, can lead to enhanced radar backscatter. We also suggest that the apparent reductions in electron density (‘bite-out’), which have been observed in the radar backscatter region, can be caused by the inability of an electrostatic probe to deflect the massive dust particles. The dust density which is required by our model to explain radar backscatter and electron bite-outs is of the order of 10 cm −3 for dust of radius above 5 × 10 −6 cm.

PKE-Nefedov: plasma crystal experiments on the International Space Station

The plasma crystal experiment PKE-Nefedov, the first basic science experiment on the International Space Station (ISS), was installed in February 2001 by the first permanent crew. It is designed for long-term investigations of complex plasmas under microgravity conditions. `Complex plasmas contain ions, electrons, neutrals and small solid particles - normally in the micrometre range. These microparticles obtain thousands of elementary charges and interact with each other via a `screened Coulomb potential. Complex plasmas are of special interest, because they can form liquid and crystalline statesxa0(Thomas et al 1994 Phys.xa0Rev.xa0Lett.xa073 652-5, Chu and I 1994 Phys.xa0Rev.xa0Lett.xa072 4009-12) and are observable at the kinetic level. In experiments on Earth the microparticles are usually suspended against gravity in strong electric fields. This creates asymmetries, stresses and pseudo-equilibrium states with sufficient free energy to readily become unstable. Under microgravity conditions the microparticles move into the bulk of the plasmaxa0(Morfill et al 1999 Phys.xa0Rev.xa0Lett.xa083 1598), experiencing much weaker volume forces than on Earth. This allows investigations of the thermodynamics of strongly coupled plasma states under substantially stress-free conditions. In this first paper we report our results on plasma crystals, in particular the first experimental observations of bcc lattice structures.

Restoration of Sensitivity in Chemo — Resistant Glioma Cells by Cold Atmospheric Plasma

Glioblastoma (GBM) is the most common and aggressive brain tumor in adults. Despite multimodal treatments including surgery, chemotherapy and radiotherapy the prognosis remains poor and relapse occurs regularly. The alkylating agent temozolomide (TMZ) has been shown to improve the overall survival in patients with malignant gliomas, especially in tumors with methylated promoter of the O6-methylguanine-DNA-methyltransferase (MGMT) gene. However, intrinsic and acquired resistance towards TMZ makes it crucial to find new therapeutic strategies aimed at improving the prognosis of patients suffering from malignant gliomas. Cold atmospheric plasma is a new auspicious candidate in cancer treatment. In the present study we demonstrate the anti-cancer properties of different dosages of cold atmospheric plasma (CAP) both in TMZ-sensitive and TMZ-resistant cells by proliferation assay, immunoblotting, cell cycle analysis, and clonogenicity assay. Importantly, CAP treatment restored the responsiveness of resistant glioma cells towards TMZ therapy. Concomitant treatment with CAP and TMZ led to inhibition of cell growth and cell cycle arrest, thus CAP might be a promising candidate for combination therapy especially for patients suffering from GBMs showing an unfavorable MGMT status and TMZ resistance.

Complex plasma laboratory PK-3 Plus on the International Space Station

PK-3 Plus is the second-generation laboratory for the investigation of complex plasmas under microgravity conditions on the International Space Station (ISS). It has more advanced hardware, software and diagnostics than its precursor PKE-Nefedov (Nefedov et al 2003 New J. Phys. 5 33). The first experiments with PK-3 Plus show the perfect functioning of the apparatus and provide much better insights into the properties of complex plasmas. In particular, the void in the center of the complex plasma cloud can now be easily closed, thus providing a much better homogeneity of the complex plasma—a feature which was hardly achievable before—but which is essential for many precision studies. Moreover, the use of the function generator at frequencies above the dust plasma frequency provides many possibilities for future experiments. Other interesting phenomena are related to high densities of the microparticles in the complex plasma. These so-called heartbeat and filamentary mode instabilities can be investigated in detail, by comparing particle motion with the discharge glow characteristics.

Dynamics of Lane Formation in Driven Binary Complex Plasmas

The dynamical onset of lane formation is studied in experiments with binary complex plasmas under microgravity conditions. Small microparticles are driven and penetrate into a cloud of big particles, revealing a strong tendency towards lane formation. The observed time-resolved lane-formation process is in good agreement with computer simulations of a binary Yukawa model with Langevin dynamics. The laning is quantified in terms of the anisotropic scaling index, leading to a universal order parameter for driven systems.