G.F. Carneiro
Universidade Federal Rural de Pernambuco
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Featured researches published by G.F. Carneiro.
Zygote | 2014
Gabriel Ribas Pereira; Pedro Lorenzo; G.F. Carneiro; Sylvie Bilodeau-Goeseels; J.P. Kastelic; A. Esteller-Vico; M. López-Béjar; I. K. M. Liu
Immature oocytes synthesize a variety of proteins that include the enzyme glucose-6-phosphate dehydrogenase (G6PDH). Brilliant cresyl blue (BCB) is a vital blue dye that assesses intracellular activity of G6PDH, an indirect measure of oocyte maturation. The objective was to evaluate the BCB test as a criterion to assess developmental competence of equine oocytes and to determine if equine growth hormone (eGH) enhanced in vitro maturation (IVM) of equine oocyte. Cumulus-oocytes complexes (COCs) were recovered by aspirating follicles <30 mm in diameter from abattoir-derived ovaries and were evaluated morphologically. Thereafter, COCs were exposed to BCB (26 μM) for 90 min at 39°C and selected based on the colour of their cytoplasm (BCB positive/BCB+ or BCB negative/BCB-). The COCs were allocated as follows: (a) IVM medium; (b) eGH group; (c) BCB-/IVM; (d) BCB+/IVM; (e) BCB-/eGH; and (f) BCB+/eGH. Then, COCs were cultured in vitro for 30 h, at 39°C in a 5%CO2 humidified air atmosphere. Cumulus-free oocytes were incubated in 10 μg/ml of bis-benzamide for 20 min at 39°C and nuclear maturation was evaluated with epifluorescence microscopy. Of the 39 COCs selected morphologically and subjected to BCB staining, 18/39 (46.2%) were classified as BCB+ and 21/39 (53.8%) as BCB- (P > 0.05). Maturation was not affected significantly by BCB classification, but the maturation rate was higher for oocytes that had been exposed to exogenous eGH versus controls (16/28, 57.1% versus 8/26, 30.8%, P < 0.05). In the present study, the BCB test was not useful for predicting competent equine oocytes prior to IVM. However, eGH enhanced equine oocyte maturation in vitro.
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia | 2013
L.O. Barros; Sildivane Valcácia Silva; Felipe Costa Almeida; Ellen Cordeiro Bento da Silva; G.F. Carneiro; M.M.P. Guerra
Ejaculates (n=25) of horses were used to assess the effect of glutathione peroxidase (GPx) and cysteine on the viability of frozen sperm cells. Semen was extended at Botu Crio with antioxidants, and divided in groups: G1, control; G2, 1 U GPx; G3, 5U GPx; G4, 0.5mM cysteine and G5, 1mM cysteine, packed in 0.5mL straws, and frozen. After thawing (37° C for 30 seconds) samples were analyzed for plasma membrane (IMP) and acrosome integrity (IAc), mitochondrial membrane potential (MMP) and kinematic, at zero (T0) and 60 minutes after (T60). GPx 5U and cysteine 0.5mM increased (P<0.05) IAc at T0, when compared to T60. Cysteine 1mM resulted in a higher (P<0.05) IAc on T60, than GPx 1 and 5U, and cysteine 0.5mM. The PMM from a stallion on T60 was higher (P<0.05) than those of two stallions. In sperm kinematic, VCL and VAP were higher (P<0.05) at T0 compared to T60 for the control group, and one stallion showed larger (P<0.05) kinematic values than other animals. It is concluded that the addition of glutathione peroxidase at concentrations 1U and 5U, and cysteine, at concentrations of 0.5mM and 1mM, does not interfere with the integrity of cryopreserved equine sperm, but preserves the kinetic parameters VCL and VAP after 60 minutes of incubation. It should be noted also that the stallion has a strong influence on sperm characteristics post-freezing.
Small Ruminant Research | 2011
M.M.P. Guerra; S.V. Silva; A.M. Batista; Z.F. Coleto; E.C.B. Silva; P.L.J. Monteiro; G.F. Carneiro
Journal of Equine Veterinary Science | 2014
C.E. Peña-Alfaro; L.O. Barros; G.F. Carneiro; M.O. Gastal; E.L. Gastal
Ciência Animal Brasileira | 2010
Sildivane Valcácia Silva; G.F. Carneiro; Maria Madalena Pessoa Guerra; Oswaldo Christiano Gomes Neto; André Mariano Batista; Aurea Wischral
Journal of Equine Veterinary Science | 2014
E.L. Gastal; L.O. Barros; G.F. Carneiro; M.O. Gastal
Journal of Equine Veterinary Science | 2012
L.O. Barros; Silva; G.F. Carneiro
Journal of Equine Veterinary Science | 2018
G.F. Carneiro; Jorge Eduardo Cavalcante Lucena; Lawrence de Oliveira Barros
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia | 2017
M.C.R. Medeiros; A. Farinasso; I.H.A.V. Nery; H.M. Souza; João Luís de Albuquerque Vasconcelos; Keity Laiane Gomes Trindade; André Mariano Batista; H. C. Manso Filho; G.F. Carneiro; A. Wischral
Revista de Ciências Agroveterinárias | 2015
Mábio Silvan José da Silva; Rodrigo da Silva Lima; Messias José dos Santos Silva; Jorge Eduardo Cavalcante Lucena; G.F. Carneiro; Willian Gonçalves do Nascimento; Clóves Cabreira Jobim