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Featured researches published by G.G. Schmitz.


Molecular Genetics and Genomics | 1988

Regulation of a transport operon promoter in Salmonella typhimurium: identification of sites essential for nitrogen regulation.

G.G. Schmitz; Kishiko Nikaido; Giovanna Ferro-Luzzi Ames

SummaryThe promoter of nitrogen-regulated transport, argTr, has been mutationally altered in order to determine the features that are essential for its response to nitrogen availability. Deletions of all sequences upstream of position-44 or downstream of position +2 had no effect no nitrogen regulation of argTr. These deletions define a small region of 44 bp where all necessary features for nitrogen regulation are located. This region includes for nitrogen regulation are located. This region includes sequences highly homologous to the nif consensus promoter. Alteration of this particular sequence caused drastic changes in the response to changes of nitrogen availability, thus indicating that they are directly involved in regulation. This implies that the NtrC protein must also act within this small region of the promoter. The data are discussed in terms of current-hypotheses concerning nitrogen regulation. In addition, we have shown 1. that carbon regulation at this promoter must occur at a site upstream from the nitrogen promoter; 2. that nifA can replace ntrC in the regulation of argTr.


Archive | 1992

The Digoxigenin: Anti-Digoxigenin (DIG) System

Christoph Kessler; Hans-Joachim Höltke; Rudolf Seibl; G.G. Schmitz; Thomas Walter; Rüdiger Rüger; Gregor Sagner; Josef Burg; Klaus Mühlegger; Anton Haselbeck; Wolfgang Hösel

The digoxigenin:anti-dioxigenin (DIG) indicator system is based on the specific interaction between the cardenolide steroid DIG, a chemically derived aglycon of digoxin and lanatoside C (see figure), and a high-affinity, DIG-specific antibody (Kessler, 1991).


Gene | 1990

RleAI: a novel class-IIS restriction endonuclease from Rhizobium leguminosarum recognizing 5′-CCCACA(N)12-3′3′-GGGTGT(N)9-5′

Zdenko Vesely; Armin Müller; G.G. Schmitz; Klaus Kaluza; Michael Jarsch; Christoph Kessler

Abstract The novel class-IIS restriction endonuclease, Rle AI, has the 5′-CCCACA(N) 12 -3′ 3′-GGGTGT(N) 9 -5′ , specificity.


Analytical Biochemistry | 1991

Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase

G.G. Schmitz; Thomas Walter; Rudolf Seibl; Christoph Kessler


Nucleic Acids Research | 1991

Cleavage of yeast and bacteriophage T7 genomes at a single site using the rare cutter endonuclease I-Sce I

Agneè; s Thierry; Arnaud Perrin; Jeanne Boyer; Cécile Fairhead; Bernard Dujon; B. Frey; G.G. Schmitz


Archive | 1989

Process for the detection of nucleic acids

Hans-Joachim Höltke; Rudolf Seibl; G.G. Schmitz; Hans Robert Dr. Schöler; Christoph Kessler; Ralf Mattes


Nucleic Acids Research | 1990

SgrAl, a novel class-II restriction endonuclease from Streptomyces griseus recognizing the octanucleotide sequence 5′-CR/CCGGYG-3′

N. Tautz; Klaus Kaluza; B. Frey; M. Jarsch; G.G. Schmitz; C. Kessler


Gene | 1988

Ksp632I, a novel class-IIS restriction endonuclease from Kluyvera sp. strain 632 with the asymmetric hexanucleotide recognition sequence: 5'-CTCTTC(N)1-3' 3'-GAGAAG(N)4-5'

Bryan J. Bolton; G.G. Schmitz; Michael Jarsch; Michael J. Comer; Christoph Kessler


Nucleic Acids Research | 1992

Swal, a unique restriction endonuclease from Staphylococcus warneri, which recognizes 5′-ATTTAAAT-3′

M. Lechner; B. Frey; Frank Laue; J. Anton-Botella; Cassandra L. Smith; Waltraud Ankenbauer; G.G. Schmitz


Nucleic Acids Research | 1990

The selective inhibitory effect of netropsin on relaxation of sequence specificity of restriction endonuclease SgrAI recognizing the octanucleotide sequence 5′-CR—CCGGYG-3′

Frank Laue; Waltraud Ankenbauer; G.G. Schmitz; C. Kessler

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