G. Lakshmi Sita

Direct somatic embryogenesis and plant regeneration from immature embryos of chilli (Capsicum annuum L.)

Abstract An in vitro regeneration protocol was developed in chilli (Capsicum annuum L.) from immature zygotic embryos via direct somatic embryogenesis without involving intermediate callus. Explants were cultured on Murashige and Skoogs (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), coconut water and high sucrose. Somatic embryos were first observed 15 days after culture. They proliferated directly from the embryonal axis and cotyledonary margins. Histological studies revealed the classical stages of embryo development. Both initiation and maturation was achieved on the same medium. Somatic embryos germinated into plantlets after placement on a sucrose medium with 1 mg/1 gibberellic acid (GA). The plants regenerated in this study showed cytological and morphological uniformity.

Agrobacterium-mediated genetic transformation in hot chilli (Capsicum annuum L. var. Pusa jwala)

A protocol for regeneration and genetic transformation has been established for chilli (Capsicum annuum L. var. Pusa jwala). High frequency regeneration of shoot buds from cotyledonary leaves was achieved with Murashige and Skoogs (MS) medium supplemented with 0.5 mg/l thidiazuron. Elongation of shoot buds and subsequent rooting was obtained on half-strength MS medium with 0.5 mg/l IAA. The Agrobacterium tumefaciens strain EHA 105 carrying a binary vector plasmid pBI 121 has been used for transformation. The cotyledonary explants from in vitro grown shoots were cocultivated for 2 days. Shoot buds were produced on the regeneration medium containing kanamycin (50 mg/l) and cefotaxime (400 mg/l). The shoot buds were elongated and rooted in the presence of kanamycin (25 mg/l). The transgenic nature of the regenerated plants were confirmed by the histochemical staining of GUS, polymerase chain reaction (PCR) and Southern hybridization analyses of nptII gene.

Differentiation of embryoids and plantlets from shoot callus of sandalwood

Callus cultures of sandalwood (Santalum album L.) were established from shoot segments and shoot tips of trees over 20 years old. Shoots were induced directly from shoot tip callus, while in shoot segments embryoids developed from the callus within 4 weeks after subculturing on to a medium supplemented with gibberellic acid (GA). Embryoids of 4–5 mm were transferred to basal medium or basal medium supplemented with low concentrations of auxin showed plantlet development.

High efficiency transformation protocol for three Indian cotton varieties via Agrobacterium tumefaciens

A protocol for consistent production of transgenic cotton plants in three Indian varieties was established utilizing Agrobacterium-mediated transformation. Shoot tip explants were transformed by cocultivation with Agrobacterium tumefaciens strain LBA 4404. The strain harbors a binary vector pBAL2 carrying the reporter gene

Triploid plants from endosperm cultures of sandalwood by experimental embryogenesis

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In vitro micropropagation of elite rosewood (Dalbergia latifolia Roxb

-glucuronidase intron (GUS-INT) and the marker gene neomycin phosphotransferase (NPTII). Regeneration potential of explants or different hormones was studied in detail. Among the different combinations of BAP and NAA tested, 0.1 mg

REGENERATION OF EGGPLANT (SOLANUM MELONGENA L.) FROM ROOT EXPLANTS

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Oral immunization of cattle with hemagglutinin protein of rinderpest virus expressed in transgenic peanut induces specific immune responses

of BAP and NAA in the medium influenced efficient regeneration of shoots by organogenesis. Shoot bud proliferation and elongation was achieved in 3i?½4 weeks time on medium supplemented with

Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum var. Pusa Ruby) with coat-protein gene of Physalis mottle tymovirus.

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Regeneration of plantlets from leaf disc cultures of rosewood: control of leaf abscission and shoot tip necrosis

. The putatively transformed shoots were harvested and placed for rooting on medium containing IBA and