G. Macchiarelli
Sapienza University of Rome
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Featured researches published by G. Macchiarelli.
Critical Care Medicine | 1998
Luigi Tritapepe; G. Macchiarelli; Monica Rocco; Francesco Scopinaro; Orazio Schillaci; Eugenio Martuscelli; Pietro M. Motta
OBJECTIVE To study human myocardial ultrastructural changes after carbon monoxide (CO) poisoning inducing reversible cardiac failure. DESIGN CASE REPORT clinical, functional and morphologic findings. SETTINGS Public university-affiliated hospital and electron microscopy laboratory. PATIENT A 25-yr-old woman with functional evidence of cardiac failure after acute CO poisoning. INTERVENTIONS Hyperbaric and intensive care treatment over 10 days. Scintigraphic and cardiac angiography with endomyocardial biopsy. MEASUREMENTS AND MAIN RESULTS Scintigraphy with 99mTc hexakis 2-methoxy-2-isobutyl isonitrile (sestaMIBI) showed an uptake defect in the left anterior descending artery territory. The cardiac angiography demonstrated a slight hypokinesis of the superior two thirds of the anterior wall and of the septal region with completely normal coronary angiograms. Electron microscopy of left ventricular biopsies showed slight ultrastructural changes in the myocytes. In addition, large glycogen deposits were mostly associated with swollen mitochondria. The patient was discharged in good clinical condition on day 10. CONCLUSIONS Presence of glycogen deposits associated with abnormal mitochondria may be signs of the incapability of myocardial cells in utilizing energy substrata. In the presence of normal myocardial perfusion, our findings are consistent with the presence of a stunned myocardium-like syndrome. Early recognition and treatment of this clinical syndrome allow the prevention of myocardial infarction.
Microscopy Research and Technique | 1997
Pietro M. Motta; G. Macchiarelli; Stefania A. Nottola; Silvia Correr
The morphology of the exocrine secretory unit of the pancreas, i.e. the pancreatic acinus, is reviewed. The histological features of the acini and their relation with the duct system are described. The acinar three‐dimensional architecture was studied by means of different ultrastructural techniques, some of which are complementary. The fine structure and morphodynamics of the acinar cells are also described. In addition, the location of the organelles in specific cytoplasmic domains and their close morphofunctional relationship with the sequential stages of secretion of the digestive enzymes are specially emphasized. Finally, morphological approaches are suggested to achieve a better comprehension of the physiological and pathological pancreatic activities whose morphodynamics need to be further elucidated or are almost totally unknown. Microsc. Res. Tech. 37:384–398, 1997.
Cells Tissues Organs | 1991
G. Macchiarelli; Giuseppe Familiari; A. Caggiati; Magliocca Fm; F.R. Riccardelli; A. Mianf; Pietro M. Motta
In order to study the morphological aspects of endothelial regeneration and vascular wall reaction after microvascular anastomosis, rat femoral arteries were sectioned and successively sutured (end-to
Archive | 1991
Akio Kikuta; G. Macchiarelli; Takuro Murakami
Oocyte development and hormone secretion are mutually related functions of the ovary. Oocytes develop and mature within developing follicles. Ovarian hormones are secreted from the stromal intersitial tissue, follicle, and corpora lutea, which are transformed from postovulatory follicles. The follicles, corpora lutea, and stroma compose the cortex of the ovary, and appear during the estrous cycle as successively transforming structures accompanied by very dynamic and continuous changes of their microvascular systems, including active angiogenesis, reconstruction, and degeneration of vessels.
Cell and Tissue Research | 1997
Stefania A. Nottola; G. Macchiarelli; Pietro M. Motta
Abstract.Ovarian angioarchitecture was studied by scanning electron microscopy of vascular corrosion casts in estrous, pseudopregnant (stimulated with human chorionic gonadotropin) and pregnant rabbits. In all samples, the proper ovarian branch of the ovarian artery (ramus ovaricus) entered the ovarian hilus near the caudal pole of the organ and ran parallel to the major axis of the hilus. The extraovarian venous drainage was formed by several vessels emptying into a distal large vein. The ramus ovaricus exhibited various degrees of coiling and branched in the medulla. The coiling of the ramus ovaricus and its ramifications were maintained in all samples. A venous meshwork and/or flat vein branches closely enveloped the arterial coils found in the hilus and outer medulla. At this level numerous arteriovenous contacts were demonstrated in all samples. The coiled arteries, prior to entering the ovarian cortex, supplied several small peripheral follicles which were drained by the hilar veins. In the cortex the coiled arteries branched in numerous thin, straight or slightly undulated arterioles which supplied developing estrous follicles and pseudopregnant corpora lutea. The arterioles supplying the pregnant corpora lutea were long, large and tightly spiraled. The venous drainage followed the modifications of the arterial supply. These data demonstrate that ovarian cycle and pregnancy induced significant changes in the cortical vessels, which adapted their structure to the temporary functional needs of the recruited follicles or corpora lutea. Hilar and medullary vessels have permanent structures that may represent morphological devices for (a) a continuous control of the blood flow (spiral arteries) and (b) a local recirculation of endocrine products (arteriovenous contacts) comparable to the ”countercurrent mechanism” previously shown to operate in ovaries of other species, but not yet found in rabbits.
Cell and Tissue Research | 1991
Enrico Vizza; U. Muglia; G. Macchiarelli; L. Baschieri; N. Pasetto; Pietro M. Motta
SummaryThe three-dimensional architecture of the human isthmic myosalpinx is directly visualized by means of scanning electron microscopy after removal of interstitial connective tissue through NaOH maceration and ultrasound microdissection. These investigations show that the myosalpinx is composed of irregularly running bundles of smooth muscle cells, changing their orientation within the myosalpinx and displaying longitudinal, oblique and circular directions. The muscular bundles anastomose and intermingle with other bundles running at different levels in the oviduct wall, and actually give rise to a wide and complex muscular network in which no distinct layers are readily discernible. These morphological data are consistent with the physiological findings that the transport of gametes and embryo in very early stages in the isthmic portion of the oviduct tube is the result of a discontinuous pattern of forward and backward movements.
Protoplasma | 1999
Pietro M. Motta; Stefania A. Nottola; Giuseppe Familiari; G. Macchiarelli; Silvia Correr; Sayoko Makabe
SummaryThe fine structure of the human cumulus oophorus has been reviewed on the basis of scanning and transmission electron microscopic observations as well as of immunofluorescence data. Tissues sampled from preovulatory ovarian follicles and cumulus-enclosed oocytes and fertilized eggs (collected from the oviduct or obtained during in vitro fertilization procedures) have been evaluated from a microtopographic and morphodynamic point of view in order to better clarify the possible role of this population of cells. In particular, the following aspects have been studied and discussed: the presence of multiple close contacts (modulated by the interposition of the zona pellucida) between the oocyte surface and the long microvillous evaginations projecting from the inner aspect of corona cells surface (through these structures the intraovarian cumulus oophorus may control oocyte growth and metabolism up until the time of ovulation); the occurrence of different subpopulations of cells (steroid-synthetic cells, cells producing adhesive proteins, leukocytes, macrophages) in the postovulatory, extraovarian cumulus oophorus surrounding oocytes, zygotes and early developing embryos. All these elements found in the cumulus mass may positively act, through their paracrine activities, on the chemical composition of the microenvironment in which fertilization occurs.
Archive | 1992
G. Macchiarelli; Stefania A. Nottola; Akio Kikuta; Osamu Ohtani; Takuro Murakami; Pietro M. Motta
The main ovarian activities are morphofunctionally related to the dynamic structure called the luteo-follicular complex (LFC). The LFC, in fact, is the morphodynamic expression of the continuous and cyclical modifications of the parenchymal ovarian components: the developing follicles and corpora lutea.
Annals of Anatomy-anatomischer Anzeiger | 2003
Isaura M.M. Prado; Liberato J.A. Di Dio; Marcílio Hubner Miranda-Neto; Sonia Lucy Molinari; Tiziana Stallone; G. Macchiarelli; Pietro M. Motta
The arrangement of the collagen bundles was studied in the Peyers patches of swine terminal ileum, by means of light microscopy (using silver-impregnation technique and picrosirius F3BA staining) and scanning electron microscopy (after NaOH-maceration). The lymphoid tissue forms a large and continuous patch along the antimesenteric border. The follicles are disposed mainly in the tela submucosa and sometimes they reached in the tunica mucosa surface (follicle/dome structures). Some follicles are located in the lamina propria of the tunica mucosa. Light microscopy showed black and brown-stained fibers, and yellow and red, and green-stained fibers, respectively by silver impregnation technique and picrosirius red staining, in the tela submucosa. In this tela, by scanning electron microscopy, the collagen fibers appeared as thick bundles forming a network of parallel layers. This network was denser in the interfollicular than in the follicular area, and formed a capsule surrounding the lymphoid follicles. Our results pointed out that a clear correspondence exists between the findings of currently used light microscopy techniques and the scanning electron microscopy after alkali-water maceration method. The arrangement of the collagen fibers in the antimesenteric border of the tela submucosa suggested a functional compartmentalization within the aggregated lymphoid follicles. This could facilitate the antigen-to-cell and cell-to-cell interaction during the immune response and thus create a suitable microenvironment for an active cell metabolism. The tunica mucosa showed a porous structure and its frequent gaps were likely the sites through which lymphocytes and other cells could freely migrate thus participating in the immunological activities of these structures.
Cells Tissues Organs | 1994
Pietro M. Motta; Enrico Vizza; Silvia Correr; G. Macchiarelli; Giuseppe Familiari; U. Muglia
The presence and distribution of contractile cells in mature corpora lutea (CL) of pregnant and normal cycling mice were studied by transmission (TEM) and scanning (SEM) electron microscopy. In the connective tissue surrounding the CL, SEM observations made after maceration in alkali, revealed long, flat, spindle-shaped elements arranged parallel to each other, forming a continuous capsule. TEM of these elements revealed ultrastructural features of mature smooth muscle cells (SMC), as well as structures intermediate between fibroblasts, fibrocytes and SMC. Discrete bundles of SMC were seen to exit the external capsule of the CL and to penetrate the luteal tissue. The extensive branches of these bundles of SMC appeared to make contact with numerous vessels. Therefore, three-dimensional reconstruction, based on TEM and SEM analysis, revealed that the SMC of the CL were organized such that they form a wide fibromuscular skeleton around and within the luteal mass. It is possible that pulsatile or tonic contraction of this structure may help to regulate the secretory activity of the luteal tissue by modifying the pressure inside the CL.