G. Manicardi
University of Modena and Reggio Emilia
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Featured researches published by G. Manicardi.
International Journal of Food Microbiology | 2001
Patrizia Messi; Moreno Bondi; Carla Sabia; Renata Battini; G. Manicardi
Lactic acid bacteria (134) from Italian sausages were tested for the production of antimicrobial substances (bacteriocins). Six percent of these showed antibacterial activity against one or several closely related microorganisms used as indicators. Lactobacillus plantarum 35d in particular produced a bacteriocin of high activity (320 AU ml(-1)) and a wide range of antimicrobial activity including S. aureus, L. monocytogenes, and A. hydrophila. The bacteriocin withstood heating at 80 degrees C for 120 min and storage at 4 degrees C for 6 months. The mode of action was identified as bactericidal. The apparent molecular weight of the bacteriocin extracted with n-butanol was estimated to be 4.5 kDa.
International Journal of Food Microbiology | 2008
Ramona Iseppi; Francesco Pilati; M. Marini; Maurizio Toselli; Simona de Niederhäusern; Elisa Guerrieri; Patrizia Messi; Carla Sabia; G. Manicardi; Immacolata Anacarso; Moreno Bondi
In this study, Enterocin 416K1, a bacteriocin produced by Enterococcus casseliflavus IM 416K1, was entrapped in an organic-inorganic hybrid coating applied to a LDPE (low-density polyethylene) film for its potential use in the active food packaging field. The antibacterial activity of the coated film was evaluated against Listeria monocytogenes NCTC 10888 by qualitative modified agar diffusion assay, quantitative determination in listeria saline solution suspension and direct contact with artificially contaminated food samples (frankfurters and fresh cheeses) stored at room and refrigeration temperatures. All investigations demonstrated that enterocin-activated coatings have a good anti-listeria activity. Qualitative tests showed a clear zone of inhibition in the indicator lawn in contact with and around the coated film. During the quantitative antibacterial evaluation the L. monocytogenes viable counts decreased to 1.5 log units compared to the control. The inhibitory capability was confirmed also in food-contact assays. In all food samples packed with coated films we observed a significant decrease in L. monocytogenes viable counts in the first 24 h compared to the control. This difference was generally maintained up to the seventh day and then decreased, with the exception of the cheese samples stored at refrigeration temperature.
International Journal of Food Microbiology | 2002
Carla Sabia; G. Manicardi; Patrizia Messi; S. de Niederhäusern; Moreno Bondi
Enterococci (118) from Italian sausages were tested for the production of antimicrobial substances. Of these, 7.6% showed antibacterial activity against one or several closely related microorganisms used as indicators. Enterococcus casseliflavus IM 416K1 in particular produced a bacteriocin (Enterocin 416K1) with strong anti-listerial antagonistic activity. The bacteriocin withstood heating at 90 degrees C for 120 min and storage at 4 degrees C for 6 months. The mode of action was identified as bactericidal. The crude activity of Enterocin 416K1 was linked to a molecule with an apparent molecular weight smaller than 5 kDa. Plasmid analysis of E. casseliflavus IM 416K1 revealed the presence of four plasmids with different molecular weights (34, 11, 7 and 3.3 MDa). All the Bac- variants produced by curing experiments showed loss of the single plasmid of 34 MDa. Bacteriocin activity and immunity production may be linked to genes located on that same plasmid.
Journal of Applied Microbiology | 2008
Carla Sabia; S. de Niederhäusern; Elisa Guerrieri; Patrizia Messi; Immacolata Anacarso; G. Manicardi; Moreno Bondi
Aim: Three hundred and two enterococci were isolated from food, animal and clinical samples in order to evaluate the incidence of vancomycin‐resistant enterococci (VRE) and bacteriocin, cytolysin, haemolysin, gelatinase production.
Current Microbiology | 2007
Simona de Niederhäusern; Carla Sabia; Patrizia Messi; Elisa Guerrieri; G. Manicardi; Moreno Bondi
Vancomycin-resistant enterococci (VRE) in healthy people and in food-producing animals seems to be quite common in Europe. The existence of this community reservoir of VRE has been associated with the massive use of avoparcin in animal husbandry. Eight years after the avoparcin ban in Europe, we investigated the incidence of VanA enterococci, their resistance patterns, and the mobility of their glycopeptide-resistance determinants in a sampling of animal rectal swabs and clinical specimens. A total of 259 enterococci isolated from equine, swine, and clinical samples were subcultured on KF-streptococcus agar (Difco Laboratories, Detroit, MI) supplemented with vancomycin and teicoplanin; 7 (6.7%), 10 (16%), and 8 (8.6%) respectively were found to be glycopeptides resistant (VanA phenotype). Slight differences in antimicrobial resistance patterns resulted among VRE recovered from the different sources.Polymerase chain reaction amplification demonstrated the presence of the vanA gene cluster and its extrachromosomal location in VRE plasmid DNA. VanA resistance was transferred in 7 out of 25 mating experiments, 4 with clinical, 2 with swine, and only 1 with equine donors. The conjugative plasmids of animal strains showed a high homology in the restriction profiles, unlike plasmids of clinical microrganisms. Our observations confirmed the possible horizontal transfer of VanA plasmids across different strains and, consequently, the diffusion of the vancomycin-resistance determinants.
Letters in Applied Microbiology | 2004
Carla Sabia; Patrizia Messi; S. de Niederhäusern; G. Manicardi; Moreno Bondi
Aims: The antimicrobial activity of two plasmid‐borne bacteriocins produced by Enterococcus casseliflavus IM 416K1 and Ent. faecalis IM 388C and their mating transferability were studied.
Letters in Applied Microbiology | 2004
Simona de Niederhäusern; Carla Sabia; Patrizia Messi; Elisa Guerrieri; G. Manicardi; Moreno Bondi
Aims: The glycopeptide‐resistance transferability from vancomycin‐resistant enterococci (VRE) of clinical and animal origin to different species of Listeria was investigated.
International Journal of Food Microbiology | 2003
Carla Sabia; S. de Niederhäusern; Patrizia Messi; G. Manicardi; Moreno Bondi
Current Microbiology | 2011
Simona de Niederhäusern; Moreno Bondi; Patrizia Messi; Ramona Iseppi; Carla Sabia; G. Manicardi; Immacolata Anacarso
Microbiologica | 1987
Fabio U; Moreno Bondi; G. Manicardi; Patrizia Messi; R. Neglia