G. Meynadier

Les protéines des corps d'inclusion des baculovirus

RésuméLa comparaison des granules ou des protéines de granules desBaculovirus dePieris brassicae, Pygaera anastomosis, Hyphantria cunea, Carpocapsa pomonella, Mythimna unipuncta, Mamestra oleracea a été entreprise par les techniques délectrophorèse, dagglutination, dimmunofluorescence et de précipitation en gel. Lélectrophorèse fait apparaître létroite ressemblance qui existe entre les protéines des six sortes de corps dinclusion. Lanalyse immunochimique montre que chaque granule est bien distinct de tous les autres; elle peut servir de base à lidentification des divers virus.SummaryThe proteins ofBaculovirus capsules ofPieris brassicae, Pygaera anastomosis, Hyphantria cunea, Mamestra oleracea, Mythimna unipuncta andCarpocapsa pomonella are studied and compared.There is no significant difference in the composition of electrophoregrams of proteins extracted from capsules of six virus strains by means of a thioglycolate buffer, pH 10,5. Two chief zones (G and F bands) are identified in every one of these strains. Mobility of proteins (C proteins) contained in the G intermediate band is slightly identical, only C proteins ofPieris brassicae andPygaera anastomosis can be differentiated according to a slight difference between electrophoretic mobility.Agglutination and immunofluorescence techniques used for suspensions and smears of granules enable to recognize granules ofC. pomonella andM. unipuncta among the six types of granules.By the immunodiffusion technique, it is revealed that F antigens occurs in all strains and C antigens in five ones. In addition to these common antigens, there are specific antigens respectively named TPa, TPb, J, U, P antigens forP. anastomosis, P. brassicae, H. cunea, M. unipuncta andC. pomonella granules.Capsules taken in Hungary and France from larvae ofC. pomonella could not be differentiated by the techniques used.This work emphasizes both characters common to the group of viruses studied and differences sufficient for identifying every one of types studied.

Les protéines des corps d'inclusion des baculovirus: IV. — Étude de sept polyédroses de Lépidoptères

La comparaison des protéines des polyèdres des Baculovirus deGalleria mellonella L.,Lymantria dispar L.,Autographa californicaSpeyer,Mamestra brassicae L.,Arctia caja L.,Bombyx mori L. etAntheraea pernyiGuer. en immunodiffusion montre quil existe entre elles une étroite parenté antigénique.Des différences entre ces protéines peuvent être mises en évidence à laide de sérums spécifiques obtenus dans des conditions particulières dimmunisation.SummaryThe proteins of Baculovirus inclusion bodies were compared by immunodiffusion inGalleria mellonella L.,Lymantria dispar L.,Autographa californicaSpeyer,Mamestra brassicae L.,Arctia caja L.,Bombyx mori L.,Antheraea pernyiGuér.Three antigenic materials were used to prepare anti-sera. A: whole non-solubilized inclusion bodies, B: purified inclusion bodies treated with CO3Na2 (B1) and with thioglycolate buffer (B2). C1 and C2: purified and treated inclusion bodies deprived of virions by centrifugation.The sera obtained by the B and C methods were not very specific but rich in anti C antibodies. showing a close relationship between the proteins of these polyhedrosis.The A immunizing method enabled us to obtain in approximately 30% of cases (serum 119.2 antiL. dispar, serum 125.2 antiA. californica, 128.2 antiA. pernyi) specific sera revealing characteristics peculiar to species. The sera may be used in the practical identification of Baculoviruses.

Les protéines des corps d’inclusion desBaculovirus 1. — Étude de leur solubilisation

RésuméL’étude immunochimique des corps d’inclusion formés par lesBaculovirus de Lépidoptères est entreprise en vue de différencier ces viroses. Le travail relaté décrit l’influence de différents solutés sur l’extraction des protéines des granules de la granulose dePieris brassicae. L’intérêt de l’emploi d’agents réducteurs tels que l’acide thioglycolique ou le mercaptoéthanol pour la solubilisation des granules est mis en évidence.SummaryThe proteins of inclusion bodies of Baculoviruses (pathogens causing granulosis and nuclear polyhedrosis) are peculiar to insect viruses. The study of their immunochemical and biochemical characteristics should make it possible to establish an accurate differential diagnosis.The present work is a preliminary study, the objective of which is to determine optimum conditions for the solubilization of inclusion bodies.The following solutions are used for dissolving granules of the granulosis ofPieris brassicae:1°CO3Na2 (0,06-0,25M)+ClNa 0,05M (Bergold’ process, 1963).2°Tris 2 M.3°Mixtures of tris and urea.4°Sodium thioglycolate-bicarbonate (0,06–0,25 M), pH 10,5.5°2-mercaptoethanol 1% in tris 1 M.nThe proteins released are analysed by immunodiffusion and starch gel electrophoresis. Two different proteins are revealed: the T protein is extracted when reducing agents are added (thioglycolic acid, 2-mercaptoethanol) in alkaline solutions; the C protein is released in any case.The use of reducing agents improves the classical process of solubilization of inclusion bodies.

Les protéines des corps d'inclusion desBaculovirus III. Étude comparée de la granulose dePieris brassicae et de la polyédrose deBombyx mori

RésuméLétude des protéines des granules dePieris brassicae L. et des polyèdres nucléaires deBombyx mori L. par les techniques de filtration sur gel, délectrophorèse et dimmunodiffusion révèle de nombreuses ressemblances. La comparaison des protéines des corps dinclusion engendrés par lesBaculovirus des Lépidoptères contribue à montrer lhomogénéité de ce groupe.SummaryIt is generally admitted that the viruses of nuclear polyhedroses and granuloses of Lepidoptera form two distinct groups serologically.A comparative study of proteins of nuclear polyhedral bodies ofBombyx mori and of granules ofPieris brassicae was carried by using techniques of gel filtration, electrophoresis and immunodiffusion. The characteristics of proteins of granules studied by gel chromatography and electrophoresis are closely related to those of proteins of polyhedral bodies.In immunodiffusion, the extraction of proteins of polyhedral inclusion bodies by the carbonate reveals a common antigen (C antigen) which is recognized by homologue serums and anti-protein heterologue serums of inclusion bodies of seven otherBaculovirus. The extraction by the thioglycolate reveals, in addition to C antigens, specific antigens: T antigen for the granulosis ofPieris brassicae and B antigens for the nuclear polyhedrosis ofBombyx mori.The comparison of proteins of inclusion bodies generated by granulosis and nuclear polyhedrosis viruses contributes to show the homogeneity of the LepidopterBaculovirus group.

Une granulose, nouvelle affection deMamestra oleracea L. [Lepidoptera, Noctuidae]

RésuméUne granulose, maladie nouvelle pourMamestra oleracea L., est décrite. Lagent pathogène se multiplie dans la région nucléaire des cellules adipeuses, hypodermiques et trachéales. Les granules et les virus ont été observés au microscope électronique. Lélevage dun nombre de générations successives relativement élevé, rendu possible par lemploi dun aliment artificiel, est envisagé comme cause dapparition de maladies à virus.SummaryMamestra (Polia) oleracea L., a noctuid of economic importance, shows a susceptibility to a very pathogenic granulosis when it is reared on an artificial medium. The pathogen develops in the nuclear area of fat body, hypoderm and trachea cells. Granules and viruses have been observed in electron microscopy.Bergoldiavirus oleraceae nov. sp. is suggested as a denomination. Rearing of many successive generations, made possible by use of artificial diet, is considered as a possible way of introduction of virus diseases in insects.

Une Granulose, nouvelle Affection deMamestra oleracea

Une granulose, maladie nouvelle pourMamestra oleracea L., est decrite. Lagent pathogene se multiplie dans la region nucleaire des cellules adipeuses, hypodermiques et tracheales. Les granules et les virus ont ete observes au microscope electronique. Lelevage dun nombre de generations successives relativement eleve, rendu possible par lemploi dun aliment artificiel, est envisage comme cause dapparition de maladies a virus.

Une Granulose, nouvelle Affection de Mamestra oleracea L. (Lepidoptera, Noctuidae)

Une granulose, maladie nouvelle pourMamestra oleracea L., est decrite. Lagent pathogene se multiplie dans la region nucleaire des cellules adipeuses, hypodermiques et tracheales. Les granules et les virus ont ete observes au microscope electronique. Lelevage dun nombre de generations successives relativement eleve, rendu possible par lemploi dun aliment artificiel, est envisage comme cause dapparition de maladies a virus.