G. R. Foxcroft

Nutritional restriction in lactating primiparous sows selectively affects female embryo survival and overall litter development

This study explored the possibility of sex-specific effects on embryonic survival in primiparous sows subjected to restricted feed intake during the last week of lactation and bred after weaning (Restrict; n = 16), compared with control sows fed close to ad libitum feed intakes (Control; n = 17). Restrict sows were in a substantial negative net energy balance at weaning, and lost 13% of estimated protein and 17% of fat mass during lactation, yet the weaning-to-oestrous interval and ovulation rate were not different between treatments. However, embryonic survival at Day 30 of gestation was lower (P < 0.05) in Restrict than Control sows, and selectively reduced the proportion of female embryos surviving (P < 0.01). A decrease in weight and crown-rump length of surviving female (P < 0.05) and male (P < 0.05) embryos was seen in Restrict litters. The mechanisms mediating this sex-specific effect on embryonic loss in feed-restricted sows are unclear. The data presented here indicate that feed-restriction during the last week of lactation in primiparous sows causes a selective decrease in survival of female embryos and limits the growth of all surviving embryos.

Biomarkers of in vivo fertility in sperm and seminal plasma of fertile stallions

The global proteome of sperm and seminal plasma of fertile stallions was investigated to determine whether associations with relative in vivo fertility exist. Seven stallions at stud in a commercial breeding station were collected throughout the breeding season and bred to a total of 164 mares to determine conception rates. On three occasions during the breeding season, raw semen was obtained from a regular collection for proteomic analysis using two-dimensional electrophoresis and also assessed for routine semen quality end points. First cycle conception rate was negatively related to ejaculate volume (r = -0.43, P = 0.05) and total IGF1 content (ng) per ejaculate (r = -0.58, P = 0.006), whereas overall pregnancy rate was positively related to sperm concentration (r = 0.56, P = 0.01). The abundance of three proteins known to be involved in carbohydrate metabolism in sperm was positively related to fertility. Furthermore, the abundance of four seminal plasma proteins were identified as being negatively related to fertility; these were identified as kallikrein-1E2 (KLK2), clusterin, and seminal plasma proteins 1 (SP1) and 2 (SP2). Abundance of cysteine-rich secretory protein 3 (CRISP3) was positively related to first cycle conception rate (r = 0.495, P = 0.027) and may provide a good marker of fertility. Based on stepwise regression analysis, clusterin and SP1 in seminal plasma together with sperm citrate synthase were predictive of fertility (r = 0.77, P < 0.0001). This study identified proteins within sperm and seminal plasma that could serve as biomarkers of semen quality and fertility in stallions.

The effect of gilt age at first estrus and breeding on third estrus on sow body weight changes and long-term reproductive performance.

The objective of this trial was to determine the effect of age at first estrus on BW changes and long-term reproductive performance of sows. At approximately 100 d of age, prepubertal C22 gilts (n = 431) were allocated to trial. At a pen average of 140 d of age, gilts began daily direct contact with mature boars to stimulate onset of puberty. Gilts (n = 317, 73%) were recorded as cyclic by 180 d of age (select) and were classified on the basis of age at puberty into 3 puberty groups: 1) early puberty (EP; <153 d of age; n = 85); 2) intermediate puberty (IP; 154 to 167 d of age; n = 140); or 3) late puberty (LP; 168 to 180 d of age; n = 90). Gilts not exhibiting the standing reflex by 180 d of age were considered nonselect (NS; n = 91). Mean day to puberty and age at puberty attainment in each of the classifications were EP: 9.6 +/- 0.5 d and 147.4 +/- 0.5 d; IP: 19.3 +/- 0.5 d and 159.9 +/- 0.3 d; LP: 33.8 +/- 0.7 and 175.7 +/- 0.6 d, respectively. Fewer NS gilts (73.0%) were bred than were EP (97.7%), IP (93.2%), or LP (93.0%) gilts (P < 0.05). Total number of piglets born and born alive were not different between classifications and increased (P < 0.05) over successive parities in EP, IP, and NS gilts. For gilts initially served, there was no effect of puberty group classification on retention in the herd to farrow a third litter, but the rate of fallout per parity tended to be greatest for NS (17.2%) compared with EP (12.4%), IP (15.6%), and LP (14.2%) gilts (P < 0.08). Taken together, these data suggest that the response to a standardized protocol of boar stimulation can identify 50 to 75% of gilts that will have greatest lifetime productivity in the breeding herd. In the known cyclic (select) gilts, BW increased over the productive life of the sow, and EP gilts were lighter than LP gilts at every measured event (P < 0.05). Plasma IGF-1 only differed between puberty groups at d 100 of age (EP: 169.0 +/- 4.4; IP: 157.2 +/- 3.5; LP: 144.0 +/- 4.4 ng/mL), suggesting a mechanism linking IGF-1 status and age at puberty in the present study.

Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars

This study investigated whether specific proteins from distinct seminal plasma fractions of boars could be related to in vivo fertility. Nine boars with acceptable sperm motility and morphology for use in artificial insemination demonstrated major differences in total number born and pregnancy rate when low sperm doses (1.5 billion sperm) were used to breed a minimum of 50 gilts per boar. The 2 lowest-fertility and 2 highest-fertility boars were chosen for evaluation of specific seminal plasma proteins. On 4 occasions, semen was collected and separated into 3 fractions based on sperm concentration (Sperm-Peak, Sperm-Rich, and Sperm-Free), and the fractions were analyzed for total protein concentration and abundance of major seminal plasma glycoprotein (PSP-I), AWN-1, and osteopontin protein using Western blotting techniques. The concentrations of these seminal plasma proteins were lower in the Sperm-Peak fractions compared with the Sperm-Free fractions (P < .05). Seminal plasma from the pooled Sperm-Rich fraction used for artificial insemination was also subjected to two-dimensional gel electrophoresis to investigate novel protein markers related to in vivo fertility. Total piglets born (r = -0.76, P = .01) and sperm motility at day 7 (r = -0.74, P = .037) were again negatively correlated with a 22-kDa protein identified by mass spectrometry as PSP-I. However, fertility index and farrowing rate tended to be positively correlated (P < .10) with a 25-kDa protein, identified as glutathione peroxidase (GPX5), an antioxidant enzyme that may protect sperm membranes from oxidative damage. These candidate proteins merit further investigation as markers of fertility in boars.

In vitro fertilization of in vitro matured pig oocytes: Effects of boar and ejaculate fraction

Ejaculates from 3 young boars were collected on 4 occasions as a series of separate 15-ml fractions. The contribution of different fractions of these ejaculates to observed variability in the quality of the semen when used for IVF was then determined. On the basis of sperm concentration, 3 fractions representing the first peak concentration (Fraction 1), the lowest sperm concentration after Fraction 1 (Fraction 2), and the second peak concentration (Fraction 3) were selected for analysis in vitro. Oocyte-cumulus-granulosa cell complexes were obtained by dissection from slaughterhouse ovaries. In vitro matured oocytes were randomly assigned for fertilization by the 3 semen samples from each boar. Sperm concentration was the same in all the samples during prefertilization incubation, while the final concentration for fertilization was 5 x 10(5) sperm/ml. Data were analysed using ANOVA for a split-plot design. In the presence of fraction effects, Student-Newman-Keuls (SNK) test was used for multiple comparison of treatment means. Oocyte penetration rates differed among fractions (P = 0.001) and varied from 69 to 100% (mean 95.7%) for Fraction 1, from 0 to 100% (mean 53.3%) for Fraction 2, and from 50to 100% (mean 89.9%) for Fraction 3. There were also differences in male pronuclear formation rate (P = 0.028; mean 27.6, 9.3 and 16.4% for Fractions 1, 2 and 3, respectively); in the rate of polyspermy (P = 0.0001; mean 92.3, 31.9 and 76.3% for Fractions 1, 2 and 3, respectively); and in the number of penetrated spermatozoa per oocyte P = 0.002; mean 5.58, 1.94 and 4.07 for Fractions 1, 2, and 3, respectively). The first peak concentration of semen (Fraction 1) showed superiority in fertilizing ability and less variability in penetration rate from replicate to replicate compared with the other 2 fractions. By multiple comparison, Boar 1 showed higher rates of penetration (P < 0.05), male pronuclear formation (P < 0.05) and polyspermy (P < 0.05) than the other 2 boars. There was no fraction-by-boar interaction. The IVM-IVF system adopted proved to be a promising method for boar semen evaluation.

Responses to delayed estrus after weaning in sows using oral progestagen treatment

Oral progestagen treatment extends the weaning-to-estrus interval (WEI) in weaned sows. Particularly in lower parity sows, this allows recovery from lactational catabolism and improves sow productivity. However, the optimal duration of progestagen treatment in contemporary dam-line sows is unclear. Therefore, sows (n = 749) weaned over consecutive 3-wk periods in June and July and classified as parity 2 and 3 (P2-3); 4, 5, and 6 (P4-6); or parity 7 or higher (P7+) were organized into 2 breeding groups using 1 of 3 strategies: 1) oral progestagen for 2 d before and 12 d after weaning (M14; n = 249); 2) oral progestagen for 2 d before and 5 d after weaning (M7; n = 250); or 3) no progestagen treatment (M0; n = 250). Progestagen (altrenogest) was administered directly into the sows mouth at a dosage of 6.8 mL (15 mg of altrenogest) daily. Sows were bred using artificial insemination at first detection of estrus after weaning (M0) or altrenogest withdrawal, and every 24 h thereafter, until they no longer exhibited the standing reflex. The WEI for M0 sows was 5.1 +/- 0.1 d. Estrus was recorded sooner (P < 0.001) after withdrawing treatment in M14 than in M7 sows (6.9 +/- 0.1 vs. 7.4 +/- 0.1 d, respectively). More (P < 0.001) M14 sows (88.6 +/- 2.5%) were bred within 10 d of altrenogest withdrawal than M7 (72.8 +/- 2.8%) sows, or within 10 d of weaning in M0 sows (78.8 +/- 2.6%). Reproductive tracts were recovered after slaughter at d 30 or 50 of gestation. For P2-3 sows, ovulation rate (least squares mean +/- 95% confidence interval) in M7 (23.1 +/- 1.0) was greater (P < 0.001) than in M14 (20.7 +/- 1.0) or M0 (19.7 +/- 1.0) sows; no differences were detected in P4-6 and P7+ sows. At d 30, M7 and M14 sows had more (P < 0.01) embryos (16.4 +/- 0.6 and 15.8 +/- 0.4, respectively) than M0 (13.9 +/- 0.5) sows. At d 50 of gestation, number of fetuses in M14 sows (13.6 +/- 0.4) was greater (P < 0.001) than in M0 (11.8 +/- 0.4) and M7 (12.2 +/- 0.3) sows. Use of oral progestagen to delay the return to postweaning estrus for greater than 18 d appears to have potential for improving weaned sow productivity. Given the incidence of high ovulation rates and associated evidence of intrauterine crowding of embryos around d 30 of gestation, the changing dynamics of prenatal loss resulting from longer periods of progestagen treatment may represent an additional production advantage.

Semen dilution for assessment of boar ejaculate quality in pig IVM and IVF systems

Use of high sperm concentrations for IVF results in high rates of penetration and polyspermy. To optimize the use of IVM-IVF for comparisons of semen quality, we determined the effect of using decreasing sperm concentrations on penetration, polyspermy and male pronuclear (MPN) formation rates and average number of spermatozoa per oocyte in standardized ejaculate fractions from 3 adult boars of different breeds. Raw semen values of the 3 boars during the period of IVF experiment were recorded for analysis. Standardized aliquots of the first sperm-rich fraction were collected (main plot) on 4 occasions. Each semen sample was serially diluted to 5 × 105, 2.5 × 105, 1.25 × 105, 6.25 × 104 and 3.125 × 104 sperm cell/ml for IVF (sub-plot). Oocyte-cumulus cell complexes collected by aspiration from slaughterhouse ovaries were cultured in vitro using standard procedures. Penetration, polyspermy and MPN formation rates were significantly affected by boars (all P < 0.001), semen dilutions (all P < 0.001) and their interactions (all P < 0.05). Penetration rates were lower (P < 0.05) for the 5 semen dilutions in Boar C (LSM 69.5, 58, 44.3, 25 and 10%) compared with Boar A (LSM 100, 100, 100, 97.5 and 92%) and Boar B (LSM 100, 94.7, 100, 92.8 and 88.3%); these differences were highly correlated to sperm motility assessed at Day 7 after semen collection. Semen dilution in Boar C resulted in a significant decrease in penetration rate but had no effect in Boars A and B over the range of dilutions used. Conversely, although semen dilution in Boars A and B decreased (P < 0.05) rate of polyspermy (LSM 100, 100, 78, 60.8, and 32.2%; and 100, 97.7, 64.3, 37.8, and 34.5%, respectively), and average number of spermatozoa per oocyte (LSM 9.1, 5.8, 3.7, 2.3, and 1.4; and LSM 8, 5.1, 3.1, 1.9 and 1, respectively), there was no effect in Boar C. Because of these interaction effects, the optimal sperm:oocyte ratio for maximal MPN formation rate was different among boars. In conclusion, serial dilution of semen used in the IVM/TVF systems effectively discriminates semen quality. Together with Day 7 motility estimates, this may allow effective prediction of relative boar fertility.

Intra-uterine growth retardation affects birthweight and postnatal development in pigs, impairing muscle accretion, duodenal mucosa morphology and carcass traits.

The present study investigated the occurrence of intra-uterine growth retardation (IUGR) in newborn (n=40) and 150-day-old (n=240) pigs of different birthweight ranges (high, HW: 1.8-2.2kg; low, LW: 0.8-1.2kg) from higher-parity commercial sows and its impact on their subsequent development and carcass traits in a Brazilian commercial production system. HW newborn pigs had heavier organs than LW pigs (P<0.01), and all brain:organ weight ratios were higher (P<0.01) in LW compared with HW offspring, providing strong evidence of IUGR in the LW piglets. HW pigs had higher bodyweights and average daily gain (ADG) in all phases of production (P<0.05), but ADG in the finisher phase was similar in both groups. Additionally, LW newborn and 150-day-old pigs showed a lower percentage of muscle fibres and a higher percentage of connective tissue in the semitendinosus muscle, greater fibre number per mm(2) and a lower height of the duodenal mucosa (P<0.05). On the other hand, HW pigs had higher hot carcass weight, meat content in the carcass and yield of ham, shoulder and belly (P<0.01). Hence, lower-birthweight piglets may suffer from IUGR, which impairs their growth performance, muscle accretion, duodenal mucosa morphology and carcass traits.

Opioid modulation of the effects of repeated stress on ACTH, cortisol, prolactin and growth hormone in pigs

Prepubertal gilts (n = 16) were restrained with a nose snare for 15 min each day over 9 days. At the beginning of the first and last nose snare, the animals were also injected IV with 1 mg/kg of naloxone. Blood samples were taken before and after restraint at 15-min intervals and plasma assayed for ACTH, cortisol, prolactin, and GH. The initial restraint led to significant increases in ACTH, cortisol, prolactin, and GH concentrations. There was no evidence of a reduction in the magnitude of endocrine responses with repeated restraint. Indeed, the response of GH was more apparent after the final restraint. In the absence of the restraint, naloxone elevated cortisol and ACTH concentrations. Naloxone, given with the first restraint, enhanced the increase in ACTH, cortisol, and prolactin. Naloxone, given during the last restraint, inhibited the increase in GH, but had less effect upon cortisol and prolactin concentrations than during the initial restraint. Gilts genetically selected for a high cortisol response to ACTH injections had a higher basal cortisol concentration and a higher cortisol response to restraint than gilts selected for a low cortisol response to ACTH. However, there were no differences between these groups in ACTH, prolactin, or GH concentrations, or in any endocrine response to naloxone. Endogenous opioids can inhibit pituitary-adrenocortical responses and enhance GH responses of pigs to stress. Measures of cortisol concentrations are poor predictors of prolactin and GH responses to stress.

Nutrition and reproduction in the pig: Ovarian aetiology

Abstract All mammalian reproductive processes will ultimately be determined by nutrient availability, from gametogenesis to lactation. The modern production gilt and sow, selected for lean growth rate and optimal milk production over an abbreviated lactation, represent extreme models in which to investigate interactions between the demands of somatic growth and reproduction. The focus of this review is nutritional modulation of the porcine gonad and, more specifically, the ovary, rather than the entire hypothalamo-hypophysial-gonadal axis. The influences and mechanisms of action of metabolic hormones and growth factors of both extra- and intra-ovarian origin are considered. Additionally, regulation of circulating gonadal steroids by nutrition and the consequent implications for gonadotrophin secretion and embryo mortality are discussed.