G. Raveendra Babu

Bioanalytical Method Development and Validation for Simultaneous Estimation of Paracetamol and Cefixime by using RP-HPLC in Rabbit Plasma

A novel approach was used to develop and validate a bioanalytical RP-HPLC method for the simultaneous estimation of Paracetamol and Cefixime in rabbit plasma using Cefaclor as internal standard. Evaluation of the content of drugs were done by employing a mixture of Phosphate buffer (p H 6.4) and Acetonitrile (80:20, v/v) as the mobile phase and measure the absorbance at 245 nm for Paracetamol and Cefixime. Retention time was established to be 3.618 min for Cefaclor, 4.608 min for Paracetamol and 5.914min for Cefixime. The results shown that the analytical technique furnished here establishes acceptable accuracy and precision, shorter and easy sample preparation, reduced the complications for equipment on satisfactory analysis time.

Visible Spectroscopic Method for Estimation of Atenolol in Tablets

A simple, accurate, cost effective and reproducible spectrophotometric method has been developed for the estimation of atenolol in tablets. The method was based on the formation of colored chromogen (vanilline). The λ-max of atenolol was found to be 650nm to both crude and marketed sample and is analyzed using the beerlamberts law. The percentage of recovery of atenolol ranged from (99.5 ± 0.16) in pharmaceutical dosage form. The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. Beers law was obeyed in the concentration range of 2–10μg/ml having line equation Y=0. 2071C-0.0043 with a correlation coefficient of 0.9999. The results of the analysis were validated statistically and by recovery study.