G. V. Marinetti

Paper chromatography of phospholipides with solvent mixtures of ketones and acetic acid.

Abstract Mixtures of ketones and acetic acid, namely 2-pentanone-acetic acid 30:2, 3-methyl-2-butanone-acetic acid 30:3, 4-methyl-2-pentanone-acetic acid 30:2, and 2, 6-dimethyl-4-heptanone-acetic acid 30:7 were found to give excellent separations of individual phospholipides on paper. It was possible to separate lysolecithin, sphingomyelin, phosphatidyl ethanolamine, lecithin, and phosphatidic acid in a unidimensional chromatogram. The individual phospholipides had to be present at concentrations from 10 to 25 /μg./20 μl. in order to achieve good separations.