Gab Sang Lee
Seoul National University
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Featured researches published by Gab Sang Lee.
Biology of Reproduction | 2004
So Hyun Lee; Dae-Young Kim; Dong Hyun Nam; Sang Hwan Hyun; Gab Sang Lee; Hye Soo Kim; Chang Kyu Lee; Sung Keun Kang; Byeong Chun Lee; Woo Suk Hwang
Abstract Platelet activating factor (PAF) is known as an autocrine growth/survival factor in mammalian preimplantation embryos. This study investigated the expression of porcine PAF receptor (PAFr) mRNA and its role in porcine in vitro fertilized (IVF) or somatic cell nuclear transfer (SCNT) embryo development. The expression of PAFr mRNA in IVF or SCNT blastocysts was shown by reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis. Semiquantitative RT-PCR and Southern blot analysis demonstrated that PAFr mRNA was expressed during preimplantation embryo development, it was highly expressed through the 2-cell to 8-cell embryo stage, and it decreased at the morula stage. PAFr mRNA expression was detected steadily in IVF embryos, whereas it was varied at the 2-cell, 4-cell, and blastocyst stages in SCNT embryos. To determine the role of PAF in IVF and SCNT embryo development, embryos were cultured in North Carolina State University (NCSU)-23 medium supplemented with different concentrations of PAF (0, 0.037, 0.37, 3.72, or 37.2 nM). The PAF supplement significantly increased the rate of blastocyst formation in SCNT embryos, but not in IVF embryos. The PAF supplement for the entire 168 h of culture showed significantly higher blastocyst formation in SCNT embryos. Upregulation of PAFr mRNA by PAF in SCNT embryos indicated that the embryotrophic effect of PAF was mediated through its functional receptors in SCNT embryos. In conclusion, the present study demonstrated that PAFr mRNA was expressed in porcine IVF and SCNT embryos, and that PAF supplement improved the developmental competence of SCNT embryos through its specific receptors.
Theriogenology | 2005
Gab Sang Lee; Hye Soo Kim; Sang Hwan Hyun; So Hyun Lee; Hyun Yong Jeon; Dong Hyun Nam; Yeon Woo Jeong; Sue Kim; Ji Hye Kim; Jae Yong Han; Curie Ahn; Sung Keun Kang; Byeong Chun Lee; Woo Suk Hwang
Theriogenology | 2004
Hye Soo Kim; Gab Sang Lee; Sang Hwan Hyun; So Hyun Lee; Dong Hyun Nam; Yeon Woo Jeong; Sue Kim; Sung Keun Kang; Byeong Chun Lee; Woo Suk Hwang
Molecular Reproduction and Development | 2005
Sue Kim; Gab Sang Lee; So Hyun Lee; Hye Soo Kim; Yeon Woo Jeong; Ji Hye Kim; Sung Keun Kang; Byung Chun Lee; Woo Suk Hwang
Molecular Reproduction and Development | 2005
Gab Sang Lee; Hye Soo Kim; Sang Hwan Hyun; Hyun Yong Jeon; Dong Hyun Nam; Yeon Woo Jeong; Sue Kim; Ji Hye Kim; Sung Keun Kang; Byeong Chun Lee; Woo Suk Hwang
Molecular Reproduction and Development | 2005
Gab Sang Lee; Hye Soo Kim; So Hyun Lee; Min-Soo Kang; Dae Yong Kim; Chang Kyu Lee; Sung Keun Kang; Byeong Chun Lee; Woo Suk Hwang
Theriogenology | 2006
Hye Soo Kim; Gab Sang Lee; Ji Hye Kim; Sung Keun Kang; Byeong Chun Lee; Woo Suk Hwang
Theriogenology | 2005
Dong Hyun Nam; So Hyun Lee; Hye Soo Kim; Gab Sang Lee; Yeon Woo Jeong; Sue Kim; Ji Hye Kim; Sung Keun Kang; Byeong Chun Lee; Woo Suk Hwang
Molecular Reproduction and Development | 2008
Gab Sang Lee; Hye Soo Kim; Woo Suk Hwang; Sang-Hwan Hyun
Archive | 2012
So H. Lee; Woo S Hwang; Byeong Chun Lee; Sung K. Kang; Jek Y. Han; Jeong M. Lim; Chang K. Lee; Eun Sook Lee; Eui Bae Jeung; Jong K. Cho; Dae Y. Kim; Sang H. Hyun; Gab Sang Lee; Hye Soo Kim; Sung C. Lee; Su Cheong Yeom