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Dive into the research topics where Gabriela Obexer-Ruff is active.

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Featured researches published by Gabriela Obexer-Ruff.


Journal of Dairy Research | 2004

Strong phylogeographic relationships among three goat breeds from the Canary Islands

M. Amills; J. Capote; A. Tomás; Lucía Kelly; Gabriela Obexer-Ruff; Antonella Angiolillo; Armand Sánchez

We partially sequenced the mitochondrial D-loop region in 47 individuals from eleven Spanish and foreign goat breeds. Phylogenetic analysis of these sequences allowed us to identify a particular D-loop haplotype shared by individuals from the Palmera, Majorera and Tinerfeña Canarian breeds. Genotyping of 281 goats from 17 different breeds by PCR-Hpall RFLP evidenced that the geographical distribution of this haplotype is restricted to the Canary Islands. This ancestral mitochondrial haplotype might originate in the domestic goat herds brought by the native Canarian inhabitants approximately 3000 years ago. Although we observed other miscellaneous D-loop haplotypes in the Palmera, Majorera and Tinerfeña breeds, any of them allowed us to group individuals from these three populations in a single cluster, a feature that suggests that these haplotypes might have diverse origins. The remarkable degree of phylogeographic structure of the Canary goat breeds with regard to other Spanish and foreign populations might be attributed to the isolation of these breeds in the Canary Islands for approximately 2500 years, without exposure to the migratory movements and commercial trading events that probably affected the genesis of most domestic goat breeds worldwide. The Canarian D-loop haplotype can be efficiently genotyped by using DNA isolated from milk and cheese samples, which paves the way for the future establishment of a Canary breed identity test for these dairy products.


PLOS ONE | 2013

Accumulating Mutations in Series of Haplotypes at the KIT and MITF Loci Are Major Determinants of White Markings in Franches-Montagnes Horses

Bianca Haase; Heidi Signer-Hasler; M. M. Binns; Gabriela Obexer-Ruff; Regula Hauswirth; Rebecca R. Bellone; Dominik Burger; Stefan Rieder; Claire M. Wade; Tosso Leeb

Coat color and pattern variations in domestic animals are frequently inherited as simple monogenic traits, but a number are known to have a complex genetic basis. While the analysis of complex trait data remains a challenge in all species, we can use the reduced haplotypic diversity in domestic animal populations to gain insight into the genomic interactions underlying complex phenotypes. White face and leg markings are examples of complex traits in horses where little is known of the underlying genetics. In this study, Franches-Montagnes (FM) horses were scored for the occurrence of white facial and leg markings using a standardized scoring system. A genome-wide association study (GWAS) was performed for several white patterning traits in 1,077 FM horses. Seven quantitative trait loci (QTL) affecting the white marking score with p-values p≤10−4 were identified. Three loci, MC1R and the known white spotting genes, KIT and MITF, were identified as the major loci underlying the extent of white patterning in this breed. Together, the seven loci explain 54% of the genetic variance in total white marking score, while MITF and KIT alone account for 26%. Although MITF and KIT are the major loci controlling white patterning, their influence varies according to the basic coat color of the horse and the specific body location of the white patterning. Fine mapping across the MITF and KIT loci was used to characterize haplotypes present. Phylogenetic relationships among haplotypes were calculated to assess their selective and evolutionary influences on the extent of white patterning. This novel approach shows that KIT and MITF act in an additive manner and that accumulating mutations at these loci progressively increase the extent of white markings.


Journal of Heredity | 2008

Genetic Analysis of White Facial and Leg Markings in the Swiss Franches-Montagnes Horse Breed

Stefan Rieder; Christian Hagger; Gabriela Obexer-Ruff; Tosso Leeb; Pierre-André Poncet

White markings and spotting patterns in animal species are thought to be a result of the domestication process. They often serve for the identification of individuals but sometimes are accompanied by complex pathological syndromes. In the Swiss Franches-Montagnes horse population, white markings increased vastly in size and occurrence during the past 30 years, although the breeding goal demands a horse with as little depigmented areas as possible. In order to improve selection and avoid more excessive depigmentation on the population level, we estimated population parameters and breeding values for white head and anterior and posterior leg markings. Heritabilities and genetic correlations for the traits were high (h(2) > 0.5). A strong positive correlation was found between the chestnut allele at the melanocortin-1-receptor gene locus and the extent of white markings. Segregation analysis revealed that our data fit best to a model including a polygenic effect and a biallelic locus with a dominant-recessive mode of inheritance. The recessive allele was found to be the white trait-increasing allele. Multilocus linkage disequilibrium analysis allowed the mapping of the putative major locus to a chromosomal region on ECA3q harboring the KIT gene.


Veterinary Journal | 2010

Haematological parameters are normal in dominant white Franches-Montagnes horses carrying a KIT mutation.

Bianca Haase; Gabriela Obexer-Ruff; Gaudenz Dolf; Stefan Rieder; Dominik Burger; Pierre-André Poncet; Vincent Gerber; Judith Howard; Tosso Leeb

The KIT receptor protein-tyrosine kinase plays an important role during embryonic development. Activation of KIT is crucial for the development of various cell lineages such as melanoblasts, stem cells of the haematopoietic system, spermatogonia and intestinal cells of Cajal. In mice, many mutations in the Kit gene cause pigmentation disorders accompanied by pleiotropic effects on blood cells and male fertility. Previous work has demonstrated that dominant white Franches-Montagnes horses carry one copy of the KIT gene with the p.Y717X mutation. The targeted breeding of white horses would be ethically questionable if white horses were known to suffer from anaemia or leukopenia. The present study demonstrates that no statistically significant differences in peripheral blood parameters are detectable between dominant white and solid-coloured Franches-Montagnes horses. The data indicate that KIT mutations may have different effects in mice, pigs, and horses. The KIT p.Y717X mutation does not have a major negative effect on the haematopoietic system of dominant white horses.


Journal of Dairy Science | 2008

Sequence Analysis of Goat Major Histocompatibility Complex Class I Genes

A. Zidi; Armand Sánchez; Gabriela Obexer-Ruff; M. Amills

The polymorphism of major histocompatibility complex (MHC) class I genes has been often involved in the resistance/susceptibility to a variety of infectious and parasitic diseases. In this work, the complete sequence of the coding region of a major histocompatibility complex (MHC) class I gene in goats (Cahi-N*01701, GenBank accession no. EF569216) is reported. The length of the corresponding open reading frame was 1,077 bp encoding a mature protein of 337 amino acids. Sequencing of additional clones allowed us to identify a second locus (Cahi-NC4*50301, GenBank accession no. EF569217) that, after performing a Bayesian phylogenetic analysis, happened to cluster with a bovine non-classical MHC class I gene. Nonclassical MHC class I molecules display low levels of polymorphism and fulfill an important immunoregulatory role in the placenta to inhibit maternal rejection. This initial description of the gene content of the goat MHC class I region will contribute to the characterization, in this ruminant species, of one of the most important genetic factors in the elicitation of innate and adaptive immune responses against pathogens.


Scientific Reports | 2017

Differential distribution of Y-chromosome haplotypes in Swiss and Southern European goat breeds

O. Vidal; Cord Drögemüller; Gabriela Obexer-Ruff; Irene Reber; J. Jordana; Amparo Martínez Martínez; Valentin Adrian Bâlteanu; Juan Vicente Delgado; Shahin Eghbalsaied; V. Landi; F. Goyache; Amadou Traoré; Michele Pazzola; Giuseppe Massimo Vacca; Bouabid Badaoui; Fabio Pilla; Mariasilvia D’Andrea; I. Álvarez; J. Capote; Abdoallah Sharaf; Agueda Pons; M. Amills

The analysis of Y-chromosome variation has provided valuable clues about the paternal history of domestic animal populations. The main goal of the current work was to characterize Y-chromosome diversity in 31 goat populations from Central Eastern (Switzerland and Romania) and Southern Europe (Spain and Italy) as well as in reference populations from Africa and the Near East. Towards this end, we have genotyped seven single nucleotide polymorphisms (SNPs), mapping to the SRY, ZFY, AMELY and DDX3Y Y-linked loci, in 275 bucks from 31 populations. We have observed a low level of variability in the goat Y-chromosome, with just five haplotypes segregating in the whole set of populations. We have also found that Swiss bucks carry exclusively Y1 haplotypes (Y1A: 24%, Y1B1: 15%, Y1B2: 43% and Y1C: 18%), while in Italian and Spanish bucks Y2A is the most abundant haplotype (77%). Interestingly, in Carpathian goats from Romania the Y2A haplotype is also frequent (42%). The high Y-chromosome differentiation between Swiss and Italian/Spanish breeds might be due to the post-domestication spread of two different Near Eastern genetic stocks through the Danubian and Mediterranean corridors. Historical gene flow between Southern European and Northern African goats might have also contributed to generate such pattern of genetic differentiation.


Virology | 2006

Viral load, organ distribution, histopathological lesions, and cytokine mRNA expression in goats infected with a molecular clone of the caprine arthritis encephalitis virus.

Ana Paula Ravazzolo; Chiara Nenci; Hans-Rudolf Vogt; Andreas Waldvogel; Gabriela Obexer-Ruff; Ernst Peterhans; Giuseppe Bertoni


Vaccine | 2006

The MHC-haplotype influences primary, but not memory, immune responses to an immunodominant peptide containing T- and B-cell epitopes of the caprine arthritis encephalitis virus Gag protein

Alexandra Fluri; Chiara Nenci; Marie-Luise Zahno; Hans-Rudolf Vogt; Shiv Charan; André Busato; Gianfranco Pancino; Ernst Peterhans; Gabriela Obexer-Ruff; Giuseppe Bertoni


Journal of General Virology | 2007

Vaccination with a T-cell-priming Gag peptide of caprine arthritis encephalitis virus enhances virus replication transiently in vivo

Chiara Nenci; Marie-Luise Zahno; Hans-Rudolf Vogt; Gabriela Obexer-Ruff; Marcus G. Doherr; Reto Zanoni; Ernst Peterhans; Giuseppe Bertoni


Molecular Immunology | 2005

Nucleotide sequence and polymorphism of the caprine major histocompatibility complex class II DQA1 (Cahi-DQA1) gene

M. Amills; C. Sulas; Armand Sánchez; Giuseppe Bertoni; R. Zanoni; Gabriela Obexer-Ruff

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M. Amills

Autonomous University of Barcelona

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Armand Sánchez

Autonomous University of Barcelona

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Stefan Rieder

Bern University of Applied Sciences

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