Gabriele Grassi
International Centre for Genetic Engineering and Biotechnology
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Publication
Featured researches published by Gabriele Grassi.
Journal of Hepatology | 1995
Gabriele Grassi; Gabriele Pozzato; M. Moretti; Mauro Giacca
BACKGROUND/AIMS The determination of HCV-RNA concentration in liver samples is likely to provide interesting insights for the study of disease progression and for evaluation of the efficacy of anti-viral therapy. METHODS A procedure was developed for the precise quantification of HCV-RNA in liver biopsies, based on the competitive reverse transcription-polymerase chain reaction technology. This competitive assay consists of the co-amplification of the target RNA with known amounts of a competitor RNA molecule containing the same sequence as the target plus an insertion in the middle, allowing resolution of the two amplification products by gel electrophoresis. RESULTS The amounts of HCV-genomic-RNA and beta-actin mRNA (the latter being used as an internal standard to overcome the problem of reproducibility of quantitative RNA extraction) were evaluated in liver biopsies of 15 patients affected by hepatitis C virus-positive chronic liver disease at the time of diagnosis. All the patients underwent alpha-interferon therapy for 6 months and were subsequently followed for at least 1 further year after the end of treatment. Viral RNA concentration (which ranged from 2 to 2.7 x 10(5) HCV-RNA molecules per 10(6) beta-actin molecules) directly correlated with the efficacy of treatment, indicating that low levels of viral replication in the liver are associated with a poor response to therapy. CONCLUSIONS This study suggests that the determination of viral load in the liver is an important prognostic tool for the prediction of the efficacy of alpha-interferon therapy.
Pediatric Research | 1997
Joan C. Marini; Gabriele Grassi; Antonella Forlino
DEVELOPMENT OF RIBOZYMES AS THERAPEUTIC AGENTS FOR DOMINANT GENETIC DISEASE: Allele-Specific Cleavage of Collagen Transcripts Shows Competitive Binding Effects. 616
Nucleic Acids Research | 1997
Gabriele Grassi; Antonella Forlino; Joan C. Marini
Hepatology | 1998
Sabrina Brambilla; Giorgio Bellati; Margherita Asti; Antonella Lisa; Maria Elena Candusso; Maria D'Amico; Gabriele Grassi; Mauro Giacca; Angelo Franchini; Savino Bruno; G. Ideo; Mario U. Mondelli; Enrico Maria Silini
Nucleic Acids Research | 1994
Gabriele Grassi; Lorena Zentilin; Sabrina Tafuro; Silvia Diviacco; Alessandro Ventura; Arturo Falaschi; Mauro Giacca
Italian Journal of Gastroenterology and Hepatology | 1997
L. Mazzoran; Gabriele Grassi; Mauro Giacca; U. Gerini; S. Baracetti; Fanni-Canelles M; Francesca Zorat; Gabriele Pozzato
Experimental Cell Research | 1996
Lorena Zentilin; Sabrina Tafuro; Gabriele Grassi; Rodolfo Garcia; Alessandro Ventura; Francisco E. Baralle; Arturo Falaschi; Mauro Giacca
Archive | 2015
Mario Grassi; Simona Maria Fiorentino; Rossella Farra; Barbara Dapas; Gabriele Grassi
Archive | 2018
Gianluca Chiarappa; Michela Abrami; Rossella Farra; Barbara Dapas; Gabriele Grassi; Mario Grassi
Journal of Cystic Fibrosis | 2018
Michela Abrami; Massimo Maschio; Massimo Conese; Marco Confalonieri; S. Di Gioia; Barbara Dapas; F. Tonon; R. Farra; Mario Grassi; Gabriele Grassi; F. Gerin
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International Centre for Genetic Engineering and Biotechnology
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