Gao Changyou

PREPARATION OF SULFATED CHITOSAN/POLYLYSINE/TRANSFORM GROWTH FACTOR-β1 NANOPARTICLES AND THEIR ABILITY TO INDUCE CHONDROGENESIS DIFFERENTIATION OF BMSCs: PREPARATION OF SULFATED CHITOSAN/POLYLYSINE/TRANSFORM GROWTH FACTOR-β1 NANOPARTICLES AND THEIR ABILITY TO INDUCE CHONDROGENESIS DIFFERENTIATION OF BMSCs

采用共沉淀法制备了复合转化生长因子-1(TGF-1)的磺化壳聚糖/聚赖氨酸纳米粒子(SCS/PLL/TGF-1),研究了磺化壳聚糖的浓度对纳米粒子粒径和表面电位的影响.所得纳米微粒的粒径在240~290 nm、表面电位在-0.4~-1.3 mV之间可调.采用透射电子显微镜表征了磺化壳聚糖/聚赖氨酸(SCS/PLL)和SCS/PLL/TGF-1纳米粒子的形貌.通过体外骨髓间充质干细胞(BMSCs)培养实验,分别考察了SCS/PLL/TGF-1纳米粒子对BMSCs的增殖、氨基葡聚糖(GAGs)分泌和Ⅱ型胶原基因表达的影响.结果显示,相对于等量的SCS/PLL或同浓度的自由TGF-1,SCS/PLL/TGF-1纳米粒子可有效保护TGF-1的活性,促进BMSCs向软骨细胞分化,可望应用于软骨修复材料等领域的研究.