Garry E. Kiefer

Endoscopic fluorescence imaging of tissue selective lanthanide chelates

Endoscopic probes have been incorporated into a fluorescent imaging system for the remote quantitation of tissue selective markers. Resolution at the level of 114 line pairs/mm allows visualization of regions that are approximately 6 mm wide. Tissue selective markers, based on polyazamacrocyclic chelates of terbium increase contrast and can be quantified in tissues at the sub-picomole level. Minimally invasive in vivo fluorescence imaging is demonstrated. The potential for application of the system to disease diagnosis and bone graft morphology quantitation is discussed.

Imaging of lanthanide chelates for cancer detection and carinogenesis investigations

A new class of exogenous marker, based on polyazamacrocycic chelates of Tb (III) or Eu (III) have been shown to be useful for improving imaging contrast or enhanced tissue-site visualization when fluorescence detection is employed (1-5).