Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Gary García Espinosa is active.

Publication


Featured researches published by Gary García Espinosa.


Veterinaria Mexico | 2016

Análisis del genoma de un virus atípico de influenza aviar H5N2 de baja patogenicidad de origen mexicano

Giovanni Steffani Hernández; Fernando Chávez Maya; Edith Rojas Anaya; Elizabeth Loza Rubio; Gary García Espinosa

Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Steffani Hernandez G, Chavez Maya F, Rojas Anaya E, Loza Rubio E, Garcia Espinosa G. Genomic analysis of an atypical Mexican low-pathogenic H5N2 avian influenza virus. Veterinaria Mexico OA. 2016;3(2). doi: 10.21753/vmoa.3.2.363 We analysed the genome of a low-pathogenic avian H5N2 influenza virus isolated from the faeces of experimentally infected Pekin ducks and Leghorn-type chickens to determine its origin and molecular characteristics. The complete genomic sequence was determined using a Sanger-based genome sequencing method and was subsequently characterized by phylogenetic analysis and genetic comparison. The results of this study showed that 8 genomic segments corresponded to an avian influenza virus that were related with strains isolated in Mexico. Investigation of the haemagglutinin gene revealed the presence of few basic amino acids at the cleavage site and lack of a potential N-glycosylation site at position 11. The gene encoding the PB1 protein lacked PB1-F2 and the basic polymerase gene codes for PA-X. In addition, the basic polymerase gene contained the consensus ribosomal frameshifting motif TCC TTT CGT C, which is required for the expression of the PA-X. Molecular characteristics showed that the virus has features of a low-pathogenic H5 influenza virus with the exception of a potential N-glycosylation site at position 11. The genome information for this particular virus will provide a molecular map for further in vivo studies to identify why some influenza viruses can persist in chickens for long periods of time. Such information will be useful in countries such as Mexico, where the virus has been a poultry health problem since 1994 and has the potential to evolve high pathogenicity. Figure 1. Phylogenetic tree of the HA gene of A/chicken/Mexico/2007 (H5N2) Influenza A virus [ ]. The phylogenetic tree was constructed using the neighbour-joining method. The percentages of replicate trees in which the associated taxa clustered together in a bootstrap test (1,000 replicates) are shown next to the branches. The evolutionary distances were computed using the Kimura 2-parameter method. The analysis involved 101 chicken nucleotide sequences. Evolutionary analyses were conducted in MEGA 5.05. A larger triangle size represents a larger number of nucleotide sequences with genetic relationship.


Veterinaria Mexico | 2016

Genomic analysis of an atypical Mexican low-pathogenic H5N2 avian influenza virus

Giovanni Steffani Hernández; Fernando Chávez Maya; Edith Rojas Anaya; Elizabeth Loza Rubio; Gary García Espinosa

Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Steffani Hernandez G, Chavez Maya F, Rojas Anaya E, Loza Rubio E, Garcia Espinosa G. Genomic analysis of an atypical Mexican low-pathogenic H5N2 avian influenza virus. Veterinaria Mexico OA. 2016;3(2). doi: 10.21753/vmoa.3.2.363 We analysed the genome of a low-pathogenic avian H5N2 influenza virus isolated from the faeces of experimentally infected Pekin ducks and Leghorn-type chickens to determine its origin and molecular characteristics. The complete genomic sequence was determined using a Sanger-based genome sequencing method and was subsequently characterized by phylogenetic analysis and genetic comparison. The results of this study showed that 8 genomic segments corresponded to an avian influenza virus that were related with strains isolated in Mexico. Investigation of the haemagglutinin gene revealed the presence of few basic amino acids at the cleavage site and lack of a potential N-glycosylation site at position 11. The gene encoding the PB1 protein lacked PB1-F2 and the basic polymerase gene codes for PA-X. In addition, the basic polymerase gene contained the consensus ribosomal frameshifting motif TCC TTT CGT C, which is required for the expression of the PA-X. Molecular characteristics showed that the virus has features of a low-pathogenic H5 influenza virus with the exception of a potential N-glycosylation site at position 11. The genome information for this particular virus will provide a molecular map for further in vivo studies to identify why some influenza viruses can persist in chickens for long periods of time. Such information will be useful in countries such as Mexico, where the virus has been a poultry health problem since 1994 and has the potential to evolve high pathogenicity. Figure 1. Phylogenetic tree of the HA gene of A/chicken/Mexico/2007 (H5N2) Influenza A virus [ ]. The phylogenetic tree was constructed using the neighbour-joining method. The percentages of replicate trees in which the associated taxa clustered together in a bootstrap test (1,000 replicates) are shown next to the branches. The evolutionary distances were computed using the Kimura 2-parameter method. The analysis involved 101 chicken nucleotide sequences. Evolutionary analyses were conducted in MEGA 5.05. A larger triangle size represents a larger number of nucleotide sequences with genetic relationship.


International Journal of Poultry Science | 2008

Peptides from the Bursa of Fabricius Associated with Suppression of Mitogen Stimulated DNA - Synthesis in Bursa of Fabricius Cells Belong to Intracellular Proteins

Gary García Espinosa; Stefan Clerens; Lut Arckens; Gisela F. Erf; G. Tellez; Billy M. Hargis


Veterinaria Mexico | 2016

Genome analysis of a low-pathogenic H5N2 influenza virus that kills chicken embryos

Giovanni Steffani Hernández; Fernando Chávez-Maya; Edith Rojas Anaya; Elizabeth Loza Rubio; Gary García Espinosa


Clínica veterinaria: abordaje diagnóstico y terapéutico | 2016

Hemosiderosis y enfermedad por almacenamiento de hierro, padecimientos silenciosos en aves silvestres en cautiverio y sus complicaciones: reporte de dos casos

Luz del Rosario Pérez Sauza; Félix Domingo Sánchez Godoy; Gary García Espinosa


Clínica Veterinaria: abordaje diagnóstico y terapéutico | 2016

Enfermedad de Newcastle en pollitos criollos: Estudio patológico y filogenético

Norma Leticia Calderón Apodaca; Fernando Chávez Maya; Gary García Espinosa


Veterinaria Mexico | 2014

Sarcocystis sp. en zanates (Quiscalus mexicanus), tordos (Molothrus aeneus) y gorriones (Aimophila ruficauda) de México

Félix Domingo Sánchez Godoy; Fernando Chávez Maya; Adriana Méndez Bernal; Gary García Espinosa; Cristina Guerrero Molina; Néstor Ledesma Martínez; Elizabeth Morales Salinas


Veterinaria Mexico | 2014

Sarcocystis sp. parasites in the Mexican Great-tailed Grackle (Quiscalus mexicanus), Bronzed Cowbird (Molothrus aeneus), and Stripe-headed Sparrow (Aimophila ruficauda)

Félix Domingo Sánchez Godoy; Fernando Chávez Maya; Adriana Méndez Bernal; Gary García Espinosa; Cristina Guerrero Molina; Néstor Ledesma Martínez; Elizabeth Morales Salinas


Universidad y ciencia | 2013

Condición corporal de la cerceta ala azul (Anas discors) obtenida por actividad cinegética en el Estado de México

Nany Stivalis Cárdenas García; Arturo Cortés Cuevas; José Antonio Quintana López; Gary García Espinosa


Revista Mexicana de Ciencias Pecuarias | 2009

Secuencia parcial de un fragmento de ADN de patos silvestres homólogo al complejo mayor de histocompatibilidad de Gallus gallus

Sofía González Guzmán; Elizabeth Loza Rubio; Virginia León Regagnon; Gary García Espinosa

Collaboration


Dive into the Gary García Espinosa's collaboration.

Top Co-Authors

Avatar

Fernando Chávez Maya

National Autonomous University of Mexico

View shared research outputs
Top Co-Authors

Avatar

Elizabeth Loza Rubio

National Autonomous University of Mexico

View shared research outputs
Top Co-Authors

Avatar

José Antonio Quintana López

National Autonomous University of Mexico

View shared research outputs
Top Co-Authors

Avatar

Félix Domingo Sánchez Godoy

National Autonomous University of Mexico

View shared research outputs
Top Co-Authors

Avatar

Giovanni Steffani Hernández

National Autonomous University of Mexico

View shared research outputs
Top Co-Authors

Avatar

Adriana Méndez Bernal

National Autonomous University of Mexico

View shared research outputs
Top Co-Authors

Avatar

Cristina Guerrero Molina

National Autonomous University of Mexico

View shared research outputs
Top Co-Authors

Avatar

Elizabeth Morales Salinas

National Autonomous University of Mexico

View shared research outputs
Top Co-Authors

Avatar

Jaime Alonso Navarro Hernández

National Autonomous University of Mexico

View shared research outputs
Top Co-Authors

Avatar

Norma Leticia Calderón Apodaca

National Autonomous University of Mexico

View shared research outputs
Researchain Logo
Decentralizing Knowledge