Ge Shenguang

Molecular Imprinting-Chemiluminescence Sensor for the Determination of Amoxicillin

The amoxicillin-imprinted polymer was synthesized with methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linker. The binding characteristic of the imprinted polymer to amoxicillin was evaluated by equilibrium binding experiments. Using the imprinted polymer as recognition material, 3-(3′-nitrophenyl)-5(2′-sulfonylphenylazo)-rhodanine (4NRASP) was synthesized by the authors and was used as chemiluminescence (CL) reagent. A novel chemiluminescence (CL) sensor for the determination of amoxicillin was developed based on the CL reaction of amoxicillin with potassium permanganate in an acidic medium. The sensor displayed excellent selectivity and high sensitivity. The linear response range of the sensor was from 5.0 × 10−9 to 1.0 × 10−6 g · mL−1 (r = 0.9985) and the detection limit was 1.3 × 10−9 g · mL−1. The relative standard deviation for the determination of 1.0 × 10−7 g · mL−1 amoxicillin solution was 1.7% (n = 11). The sensor was applied to the determination of amoxicillin in urine samples with satisfactory results.

Flow injection chemiluminescence sensor with novel rhodanine ramification for determination of fenfluramine based on molecularly imprinted polymer.

Abstract Flow injection chemiluminescence (FI-CL) with molecularly imprinted polymer (MIP) was applied to determine fenfluramine. The fenfluramine-imprinted polymer was prepared with acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. Methyl and sulfonic group were introduced to rhodanine matrix, and a novel rhodanine ramification 3MORASP was synthesized by the author, and it was used as chemiluminescence reagent. 3-(3′-Methoxyphenyl)-5(2′-sulfonylphenylazo)-rhodanine (3MORASP), first synthesized by the authors, was used as chemiluminescence (CL) reagent. The novel flow path of FI-CL was designed, which made three merged streams of reactants injected into MIP column move through different pathways simultaneously. Fenfluramine was detected based on the reaction of fenfluramine, 3MORASP, and potassium permanganate in an acidic medium. The CL intensity was correlated linearly with the concentration of fenfluramine over the range of 1.0 × 10−7 to 5.0 × 10−6 g · mL−1, and the detection limit was 9.48 × 10−9 g · mL−1. The relative standard deviation (RSD) was 2.4% for determination of 1.0 × 10−6 g · mL−1 fenfluramine (n = 11). This method was successfully applied to the determination of fenfluramine in weight-reducing tonic.