Genevieve Foley

Isolation and Pure Cultivation of the Smaller Mouth Spirochetes by an Improved Method

Summary Details are given of a method for primary anaerobic cultivation in small plates of spirochetes from human and experimental fuso-spirochetal exudates, and for their pure subculture in plates and tubes. Successful primary plates were obtained in 23 instances out of 29 cases of human infection, and from all of 21 experimentally infected guinea pigs. Eleven strains of spirochetes have been isolated and subcultured in a short period with little difficulty.

Studies, in the Rat, of Susceptibility to Dental Caries: II. Effects on Teeth, in Mothers and Young, of Diets Deficient in Calcium and Vitamin D, During Pregnancy, Lactation, and Rearing

In the first report in this series (20), it was noted that enamel hypoplasia occurred in incisors of rats on a low-Ca vitamin-D-free diet, but that apparently only recently formed enamel was affected, molar teeth escaping the deformity. The emphasis placed by Mellanby (15) on possible relationships of enamel defects to dental caries suggested the desirability of attempting to produce defective molar-enamel in rats by dietary means. Since enamel calcification of rat molars is complete at a very early age, it appeared desirable to feed a deficient diet to mothers during pregnancy and lactation, and then to continue the young under the same dietary conditions. Dental effects of this and other conditions, embodying possible factors in caries etiology, form the basis of the present report.