Gennady I. Ruban

Investigation of morphometric parameters for granulocytes and lymphocytes as applied to a solution of direct and inverse light-scattering problems

Quantitative data on cell structure, shape, and size distribution are obtained by optical measurement of normal peripheral blood granulocytes and lymphocytes in a cell suspension. The cell nuclei are measured in situ. The distribution laws of the cell and nuclei sizes are estimated. The data gained are synthesized to construct morphometric models of a segmented neutrophilic granulocyte and a lymphocyte. Models of interrelation between the cell and nucleus metric characteristics for granulocyte and lymphocyte are obtained. The discovered interrelation decreases the amount of cell-nucleus size combinations that have to be considered under simulation of cell scattering patterns. It allows faster analysis of light scattering to discriminate cells in a real-time scale. Our morphometric data meet the requirements of scanning flow cytometry dealing with the high rate analysis of cells in suspension. Our findings can be used as input parameters for the solution of the direct and inverse light-scattering problems in scanning flow cytometry, dispensing with a costly and time-consuming immunophenotyping of the cells, as well as in turbidimetry and nephelometry. The cell models developed can ensure better interpretations of scattering patterns for an improvement of discriminating capabilities of immunophenotyping-free scanning flow cytometry.

Light scattering and morphology of the lymphocyte as applied to flow cytometry for distinguishing healthy and infected individuals.

A simple optical model of single lymphocytes with smooth and nonsmooth surfaces has been developed for healthy and infected individuals. The model can be used for rapid (in the real-time scale) solution of the inverse light-scattering problem on the basis of optical data measured by label-free flow cytometry. Light scattering patterns have been calculated for the model developed. It has been shown that the smooth and nonsmooth cells can be resolved using the intensities of the sideward- and backward-scattered light. We have found by calculations and validated by the flow cytometer experiments that intensity distributions for the cells of lymphocyte populations can be used as a preliminary signatures of some virus infections. Potential biomedical applications of the findings for label-free flow cytometry detection of individuals infected with viruses of hepatitis B or C and some others viruses are presented.

Investigation of human white blood cells: application to scanning flow cytometry

Normal human leukocytes have been investigated in suspension by methods of the specialized light microscopy. On the basis of the obtained experimental data the histograms of the size distributions of lymphocytes, their nuclei, and granulocytes are constructed.

Revealing some virus infections by lymphocyte laser scattering

Light scattering patterns have been calculated for a simple optical model constructed for single smooth and nonsmooth lymphocytes of healthy and infected individuals. It has been shown that the smooth and nonsmooth cells can be resolved using the intensities of the sideward and backward scattered light. We have found by calculations and validated by the flow-cytometer experiments that intensity distributions for the cells of lymphocyte populations can be used as a preliminary signatures of some virus infections. Potential biomedical applications of the findings for label-free flowcytometry detecting of individuals infected with viruses of hepatitises B or C and some others viruses are presented.

Development of human granulocyte morphometric model for scanning flow cytometry

Cell structure, shape, and size distribution are characterized by optical measurement of normal peripheral blood granulocytes in a cell suspension as applied to a cell discrimination by the solution of the direct and inverse light-scattering problems in scanning flow cytometry (SFC). The distribution laws of the cell and nuclei sizes are estimated. A morphometric model of a segmented neutrophilic granulocyte is constructed on the base of the gained data. An interrelation between the cell and nucleus metric characteristics is deduced. The obtained interrelation allows decreasing a number of independent parameters of the cell model to adapt it to the real-time discrimination of the cells. The findings can be used as input parameters for the solution of the direct and inverse light-scattering problems in SFC-dispensing with a costly and time-consuming immunophenotyping of the cells.

Automated multifunctional photometer

A multipurpose automated photometer designed for controlling the sharpness and noise characteristics of photolayers and their spatial homogeneity is described. The photometers specifications are given and the results are reported of the investigation of the bright strip contrast function and modulation transfer function of undeveloped course- and fine-grain laboratory-cast photographic films in the passed radiation.