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Dive into the research topics where Geoffrey J. Puzon is active.

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Featured researches published by Geoffrey J. Puzon.


Metabolomics | 2008

Metabolic profiling by ion mobility mass spectrometry (IMMS)

Prabha Dwivedi; Peiying Wu; Steve J. Klopsch; Geoffrey J. Puzon; Luying Xun; Herbert H. Hill

Ion Mobility Mass Spectrometry (IMMS) was evaluated as an analytical method for metabolic profiling. The specific instrument used in these studies was a direct infusion (DI)-electrospray ionization (ESI)—ambient pressure ion mobility spectrometer (APIMS) coupled to a time-of-flight mass spectrometer (TOFMS). The addition of an ion mobility spectrometer to a mass spectrometer had several advantages over direct infusion electrospray mass spectrometry alone. This tandem instrument (ESI-IMMS) added a rapid separation step with high-resolution prior to mass spectrometric analysis of metabolite mixtures without extending sample preparation time or reducing the high through put potential of direct mass spectrometry. Further, IMMS also reduced the baseline noise common with ESI-MS analyses of complex samples and enabled rapid separation of isobaric metabolites. IMMS was used to analyze the metabolome of Escherichiacoli (E. coli), containing a collection of extremely diverse chemical compounds including hydrophobic lipids, inorganic ions, volatile alcohols and ketones, amino and non-amino organic acids, and hydrophilic carbohydrates. IMMS data were collected as two-dimensional spectra showing both mobility and mass of each ion detected. Using direct infusion ESI-IMMS of a non-derivatized methanol extract of an E. coli culture, more than 500 features were detected, of which over 200 intracellular metabolites were tentatively assigned as E. coli metabolites. This analytical method also allowed simultaneous separation of isomeric metabolic features.


Biochemical and Biophysical Research Communications | 2002

A bacterial flavin reductase system reduces chromate to a soluble chromium(III)-NAD(+) complex.

Geoffrey J. Puzon; James N. Petersen; Arthur G. Roberts; David M. Kramer; Luying Xun

Biological reduction of carcinogenic chromate has been extensively studied in eukaryotic cells partly because the reduction produces stable chromium(III)-DNA adducts, which are mutagenic. Microbial reduction of chromate has been studied for bioremediation purposes, but little is known about the reduction mechanism. In eukaryotic cells chromate is mainly reduced non-enzymatically by ascorbate, which is usually absent in bacterial cells. We have characterized the reduction of chromate by a flavin reductase (Fre) from Escherichia coli with flavins. The Fre-flavin system rapidly reduced chromate, whereas chemical reduction by NADH and glutathione was very slow. Thus, enzymatic chromate reduction is likely the dominant mechanism in bacterial cells. Furthermore, the end-product was a soluble and stable Cr(III)-NAD(+) complex, instead of Cr(III) precipitate. Since intracellularly generated Cr(III) forms adducts with DNA, protein, glutathione, and ascorbate in eukaryotic cells, we suggest that the produced Cr(III) is primarily complexed to NAD(+), DNA, and other cellular components inside bacteria.


Environmental Science & Technology | 2015

Reduced Efficiency of Chlorine Disinfection of Naegleria fowleri in a Drinking Water Distribution Biofilm

Haylea C. Miller; Jason Wylie; Guillaume Dejean; Anna H. Kaksonen; David C. Sutton; Kalan Braun; Geoffrey J. Puzon

Naegleria fowleri associated with biofilm and biological demand water (organic matter suspended in water that consumes disinfectants) sourced from operational drinking water distribution systems (DWDSs) had significantly increased resistance to chlorine disinfection. N. fowleri survived intermittent chlorine dosing of 0.6 mg/L for 7 days in a mixed biofilm from field and laboratory-cultured Escherichia coli strains. However, N. fowleri associated with an attached drinking water distribution biofilm survived more than 30 times (20 mg/L for 3 h) the recommended concentration of chlorine for drinking water. N. fowleri showed considerably more resistance to chlorine when associated with a real field biofilm compared to the mixed laboratory biofilm. This increased resistance is likely due to not only the consumption of disinfectants by the biofilm and the reduced disinfectant penetration into the biofilm but also the composition and microbial community of the biofilm itself. The increased diversity of the field biofilm community likely increased N. fowleris resistance to chlorine disinfection compared to that of the laboratory-cultured biofilm. Previous research has been conducted in only laboratory scale models of DWDSs and laboratory-cultured biofilms. To the best of our knowledge, this is the first study demonstrating how N. fowleri can persist in a field drinking water distribution biofilm despite chlorination.


Environmental Science & Technology | 2016

Characterization of a Drinking Water Distribution Pipeline Terminally Colonized by Naegleria fowleri

Matthew J. Morgan; Samuel Halstrom; Jason Wylie; Tom Walsh; Anna H. Kaksonen; David C. Sutton; Kalan Braun; Geoffrey J. Puzon

Free-living amoebae, such as Naegleria fowleri, Acanthamoeba spp., and Vermamoeba spp., have been identified as organisms of concern due to their role as hosts for pathogenic bacteria and as agents of human disease. In particular, N. fowleri is known to cause the disease primary amoebic meningoencephalitis (PAM) and can be found in drinking water systems in many countries. Understanding the temporal dynamics in relation to environmental and biological factors is vital for developing management tools for mitigating the risks of PAM. Characterizing drinking water systems in Western Australia with a combination of physical, chemical and biological measurements over the course of a year showed a close association of N. fowleri with free chlorine and distance from treatment over the course of a year. This information can be used to help design optimal management strategies for the control of N. fowleri in drinking-water-distribution systems.


FEMS Microbiology Ecology | 2017

Comparison of biofilm ecology supporting growth of individual Naegleria species in a drinking water distribution system

Geoffrey J. Puzon; Jason Wylie; Tom Walsh; Kalan Braun; Matthew J. Morgan

Free-living amoebae (FLA) are common components of microbial communities in drinking water distribution systems (DWDS). FLA are of clinical importance both as pathogens and as reservoirs for bacterial pathogens, so identifying the conditions promoting amoebae colonisation of DWDSs is an important public health concern for water utilities. We used high-throughput amplicon sequencing to compare eukaryotic and bacterial communities associated with DWDS biofilms supporting distinct FLA species (Naegleria fowleri, N. lovaniensis or Vermamoeba sp.) at sites with similar physical/chemical conditions. Eukaryote and bacterial communities were characteristics of different FLA species presence, and biofilms supporting Naegleria growth had higher bacterial richness and higher abundance of Proteobacteria, Bacteroidetes (bacteria), Nematoda and Rotifera (eukaryota). The eukaryotic community in the biofilms had the greatest difference in relation to the presence of N. fowleri, while the bacterial community identified individual bacterial families associated with the presence of different Naegleria species. Our results demonstrate that ecogenomics data provide a powerful tool for studying the microbial and meiobiotal content of biofilms, and, in these samples can effectively discriminate biofilm communities supporting pathogenic N. fowleri. The identification of microbial species associated with N. fowleri could further be used in the management and control of N. fowleri in DWDS.


Environmental Science & Technology | 2018

Competition between Naegleria fowleri and free living amoeba colonising laboratory scale and operational drinking water distribution systems

Haylea C. Miller; Jason Wylie; Anna H. Kaksonen; David C. Sutton; Geoffrey J. Puzon

Free living amoebae (FLA), including pathogenic Naegleria fowleri, can colonize and grow within pipe wall biofilms of drinking water distribution systems (DWDSs). Studies on the interactions between various FLA species in biofilms are limited. Understanding the interaction between FLA and the broader biofilm ecology could help better predict DWDS susceptibility to N. fowleri colonization. The aim of this study was to determine if N. fowleri and other FLAs ( Naegleria, Vermamoeba, Willaertia, and Vahlkampfia spp.) cocolonize DWDS biofilm. FLAs commonly isolated from DWDSs ( N. fowleri, V. vermiformis, and N. lovaniensis) were introduced into laboratory-scale biomonitors to determine the impact of these amoebae on N. fowleris presence and viability. Over 18 months, a single viable amoebae ( N. fowleri, N. lovaniensis, or V. vermiformis) was detected in each biofilm sample, with the exception of N. lovaniensis and N. fowleri, which briefly cocolonized biofilm following their coinoculation. The analysis of biofilm and bulk water samples from operational DWDSs revealed a similar lack of cocolonization with a single FLA detected in 99% ( n = 242) of samples. Interestingly, various Naegleria spp. did colonize the same DWDS locations but at different times. This knowledge furthers the understanding of ecological factors which enable N. fowleri to colonize and survive within operational DWDSs and could aid water utilities to control its occurrence.


Environmental Science & Technology | 2017

Development of Untargeted Metabolomics Methods for the Rapid Detection of Pathogenic Naegleria fowleri

Zhihao Yu; Haylea C. Miller; Geoffrey J. Puzon; Brian H. Clowers

Despite comparatively low levels of infection, primary amoebic meningoencephalitis (PAM) induced by Naegleria fowleri is extremely lethal, with mortality rates above 95%. As a thermophile, this organism is often found in moderate-to-warm climates and has the potential to colonize drinking water distribution systems (DWDSs). Current detection approaches require days to obtain results, whereas swift corrective action can maximize the benefit of public health. Presently, there is little information regarding the underlying in situ metabolism for this amoeba but the potential exists to exploit differentially expressed metabolic signatures as a rapid detection technique. This research outlines the biochemical profiles of selected pathogenic and nonpathogenic Naegleria in vitro using an untargeted metabolomics approach to identify a panel of diagnostically meaningful compounds that may enable rapid detection of viable pathogenic N. fowleri and augment results from traditional monitoring approaches.


Biodegradation | 2018

Biodegradability of polar compounds formed from weathered diesel

Melanie C. Bruckberger; Trevor P. Bastow; Matthew J. Morgan; Deirdre Gleeson; Natasha C. Banning; Greg B. Davis; Geoffrey J. Puzon

Once released into the environment, petroleum is exposed to biological and physical weathering processes which can lead to the formation and accumulation of highly recalcitrant polar compounds. These polar compounds are often challenging to analyse and can be present as an “unresolved complex mixture” (UCM) in total petroleum hydrocarbon (TPH) analyses and can be mistaken for natural organic matter. Existing research on UCMs comprised of polar compounds is limited, with a majority of the compounds remaining unidentified and their long-term persistence unknown. Here, we investigated the potential biodegradation of these recalcitrant polar compounds isolated from weathered diesel contaminant, and the changes in the microbial community composition associated with the biodegradation process. Microcosms were used to study the biodegradability of the polar compounds under various aerobic and anaerobic conditions and the results compared against the biodegradation of fresh diesel. Under all conditions tested, the majority of the polar UCM contaminant remained recalcitrant to biodegradation. The degradation was limited to the TPH portion of the polar UCM, which represented a minor fraction of the total polar UCM concentration. Changes in microbial community composition were observed under different redox conditions and in the presence of different contaminants. This work furthers the understanding of the biodegradation and long-term recalcitrance of polar compounds formed through weathering at contaminated legacy sites.


Microbiology Australia | 2009

The impact of biofilms on water quality in long pipelines

Jason J. Plumb; Geoffrey J. Puzon; Maneesha P. Ginige

Limited water availability and increased water demand necessitates the use of long pipelines to distribute potable and non-potable water for human consumption or other purposes. The effects of microbial growth and activity on the quality of distributed water have been studied for many years, although in recent years much of this focus has shifted to understanding the effects of biofilms, rather than planktonic microorganisms, on water quality. Recently, it was estimated that 95% of all biomass in water distribution systems is in the form of pipewall biofilms, with only 5% of all biomass in the bulk water phase. Under favourable conditions, biofilms can impact water quality by increasing disinfectant demand, creating taste and odour problems, harbouring opportunistic pathogens and contributing to the potential for discoloured water events. More research is required to inform the development of guidelines for the management of biofilms in long pipelines to ensure the delivery of safe drinking water and to minimise impacts on water quality.


Environmental Science & Technology | 2005

Formation of Soluble Organo−Chromium(III) Complexes after Chromate Reduction in the Presence of Cellular Organics

Geoffrey J. Puzon; Arthur G. Roberts; David M. Kramer; Luying Xun

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Anna H. Kaksonen

Commonwealth Scientific and Industrial Research Organisation

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Jason Wylie

Commonwealth Scientific and Industrial Research Organisation

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Matthew J. Morgan

Commonwealth Scientific and Industrial Research Organisation

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Luying Xun

Washington State University

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Haylea C. Miller

Commonwealth Scientific and Industrial Research Organisation

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David C. Sutton

University of Western Australia

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Tom Walsh

Commonwealth Scientific and Industrial Research Organisation

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Don McFarlane

Commonwealth Scientific and Industrial Research Organisation

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Elise Bekele

Commonwealth Scientific and Industrial Research Organisation

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Joanne Vanderzalm

Commonwealth Scientific and Industrial Research Organisation

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