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Dive into the research topics where Geoffrey Turner is active.

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Featured researches published by Geoffrey Turner.


Nature Reviews Microbiology | 2005

Fungal secondary metabolism — from biochemistry to genomics

Nancy P. Keller; Geoffrey Turner; Joan W. Bennett

Much of natural product chemistry concerns a group of compounds known as secondary metabolites. These low-molecular-weight metabolites often have potent physiological activities. Digitalis, morphine and quinine are plant secondary metabolites, whereas penicillin, cephalosporin, ergotrate and the statins are equally well known fungal secondary metabolites. Although chemically diverse, all secondary metabolites are produced by a few common biosynthetic pathways, often in conjunction with morphological development. Recent advances in molecular biology, bioinformatics and comparative genomics have revealed that the genes encoding specific fungal secondary metabolites are clustered and often located near telomeres. In this review, we address some important questions, including which evolutionary pressures led to gene clustering, why closely related species produce different profiles of secondary metabolites, and whether fungal genomics will accelerate the discovery of new pharmacologically active natural products.


Nature | 2005

Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus.

William C. Nierman; Arnab Pain; Michael J. Anderson; Jennifer R. Wortman; H. Stanley Kim; Javier Arroyo; Matthew Berriman; Keietsu Abe; David B. Archer; Clara Bermejo; Joan W. Bennett; Paul Bowyer; Dan Chen; Matthew Collins; Richard Coulsen; Robert Davies; Paul S. Dyer; Mark L. Farman; Nadia Fedorova; Natalie D. Fedorova; Tamara V. Feldblyum; Reinhard Fischer; Nigel Fosker; Audrey Fraser; José Luis García; María José García; Ariette Goble; Gustavo H. Goldman; Katsuya Gomi; Sam Griffith-Jones

Aspergillus fumigatus is exceptional among microorganisms in being both a primary and opportunistic pathogen as well as a major allergen. Its conidia production is prolific, and so human respiratory tract exposure is almost constant. A. fumigatus is isolated from human habitats and vegetable compost heaps. In immunocompromised individuals, the incidence of invasive infection can be as high as 50% and the mortality rate is often about 50% (ref. 2). The interaction of A. fumigatus and other airborne fungi with the immune system is increasingly linked to severe asthma and sinusitis. Although the burden of invasive disease caused by A. fumigatus is substantial, the basic biology of the organism is mostly obscure. Here we show the complete 29.4-megabase genome sequence of the clinical isolate Af293, which consists of eight chromosomes containing 9,926 predicted genes. Microarray analysis revealed temperature-dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype. The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus.


Nature Biotechnology | 2007

Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

Herman Jan Pel; Johannes H. de Winde; David B. Archer; Paul S. Dyer; Gerald Hofmann; Peter J. Schaap; Geoffrey Turner; Ronald P. de Vries; Richard Albang; Kaj Albermann; Mikael Rørdam Andersen; Jannick Dyrløv Bendtsen; Jacques A. E. Benen; Marco van den Berg; Stefaan Breestraat; Mark X. Caddick; Roland Contreras; Michael Cornell; Pedro M. Coutinho; Etienne Danchin; Alfons J. M. Debets; Peter Dekker; Piet W.M. van Dijck; Alard Van Dijk; Lubbert Dijkhuizen; Arnold J. M. Driessen; Christophe d'Enfert; Steven Geysens; Coenie Goosen; Gert S.P. Groot

The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli were observed. A reconstructed metabolic network comprising 1,069 unique reactions illustrates the versatile metabolism of A. niger. Noteworthy is the large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors, and the presence of putative gene clusters for fumonisin and ochratoxin A synthesis.


ChemBioChem | 2006

The fumitremorgin gene cluster of Aspergillus fumigatus : Identification of a gene encoding brevianamide F synthetase

Shubha Maiya; Alexander Grundmann; Shu-Ming Li; Geoffrey Turner

A gene encoding a putative dimodular nonribosomal peptide synthetase (NRPS) was identified within a gene cluster of Aspergillus fumigatus, a species reported to produce fumitremorgins and other prenylated alkaloids. The gene was deleted and overexpressed in the genome reference strain Af293, and was also expressed in the naïve host Aspergillus nidulans, which lacks the equivalent gene cluster. While neither fumitremorgins nor the dipeptide brevianamide F (cyclo‐L‐Trp‐L‐Pro), an early intermediate, were detected in wild‐type and deletion strains of A. fumigatus, brevianamide F accumulated in fungal cultures following increased expression of the NRPS gene in both A. fumigatus and A. nidulans. We conclude that the gene Afu8g00170, named ftmA, encodes the NRPS brevianamide synthetase. Brevianamide F is the precursor of a variety of fungal prenylated alkaloids with biological activity, including fumitremorgins A, B and C and tryprostatin B.


ChemBioChem | 2007

Identification of a hybrid PKS/NRPS required for pseurotin A biosynthesis in the human pathogen Aspergillus fumigatus.

Shubha Maiya; Alexander Grundmann; Xiang Li; Shu-Ming Li; Geoffrey Turner

The genome sequence of Aspergillus fumigatus revealed the presence of a single hybrid polyketide synthase–non‐ribosomal peptide synthetase (PKS/NRPS) gene that is present within a cluster of five genes suggestive of its involvement in secondary metabolism. Here, we present evidence that it is required for the biosynthesis of pseurotin A, a compound with an unusual heterospirocyclic γ‐lactam structure. We have confirmed that the genome reference strain A. fumigatus Af293 produces pseurotin A, a compound previously reported to be a competitive inhibitor of chitin synthase and an inducer of nerve‐cell proliferation. Deletion or overexpression of the PKS/NRPS gene psoA in A. fumigatus leads to the absence or accumulation of pseurotin A, respectively; this indicates that this gene is essential for the biosynthesis of pseurotin A. It is likely that the first product of psoA is converted to pseurotin A by the products of other genes in this cluster.


Fungal Genetics and Biology | 2009

The 2008 update of the Aspergillus nidulans genome annotation: A community effort

Jennifer R. Wortman; Jane Mabey Gilsenan; Vinita Joardar; Jennifer Deegan; John Clutterbuck; Mikael Rørdam Andersen; David B. Archer; Mojca Benčina; Gerhard Braus; Pedro M. Coutinho; Hans von Döhren; John H. Doonan; Arnold J. M. Driessen; Pawel Durek; Eduardo A. Espeso; Erzsébet Fekete; Michel Flipphi; Carlos Garcia Estrada; Steven Geysens; Gustavo H. Goldman; Piet W.J. de Groot; Kim Hansen; Steven D. Harris; Thorsten Heinekamp; Kerstin Helmstaedt; Bernard Henrissat; Gerald Hofmann; Tim Homan; Tetsuya Horio; Hiroyuki Horiuchi

The identification and annotation of protein-coding genes is one of the primary goals of whole-genome sequencing projects, and the accuracy of predicting the primary protein products of gene expression is vital to the interpretation of the available data and the design of downstream functional applications. Nevertheless, the comprehensive annotation of eukaryotic genomes remains a considerable challenge. Many genomes submitted to public databases, including those of major model organisms, contain significant numbers of wrong and incomplete gene predictions. We present a community-based reannotation of the Aspergillus nidulans genome with the primary goal of increasing the number and quality of protein functional assignments through the careful review of experts in the field of fungal biology.


Molecular Genetics and Genomics | 1996

DELTA -(L-ALPHA -AMINOADIPYL)-L-CYSTEINYL-D-VALINE SYNTHETASE IS A RATE LIMITING ENZYME FOR PENICILLIN PRODUCTION IN ASPERGILLUS NIDULANS

J. Kennedy; Geoffrey Turner

Abstract The acvA gene from Aspergillus nidulans encoding δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase was overexpressed by replacing the wild-type acvA promoter with the ethanol dehydrogenase promoter, alcAp, from A. nidulans. The expression level of alcAp was determined using a strain in which the reporter gene, lacZ, is under the control of alcAp, and was found to be up to 100 times greater than that from the acvA promoter when induced in fermentation conditions. Penicillin yields were found to increase by as much as 30-fold when the acvA gene was overexpressed. Glucose, which strongly represses transcription from alcAp, also repressed penicillin biosynthesis in the overexpression strain. These results prove that ACV synthetase is a rate limiting enzyme for penicillin production in A. nidulans.


Lancet Infectious Diseases | 2002

Sequencing the Aspergillus fumigatus genome

David W. Denning; Michael J. Anderson; Geoffrey Turner; Jean Paul Latgé; Joan W. Bennett

Aspergillus fumigatus is the most common mould pathogen of human beings and unusually causes both invasive disease in immunocompromised patients and allergic disease in patients with atopic immune systems. 4% of patients dying in modern European teaching hospitals have invasive aspergillosis and it is the leading infectious cause of death in leukaemia and bone marrow transplant patients. Until 2001, only two licensed antifungal drugs were available to treat aspergillosis-amphotericin B and itraconazole. Its 28-30Mb genome is being sequenced in an international collaboration, with the Wellcome Trust Sanger Institute (UK) and The Institute for Genomic Research (TIGR, USA) as the two main centres. A whole-genome shotgun approach was adopted and initiated in 2001 with an expected completion date in 2003. The complete sequence will permit identification of pathways specific to pathogenic Aspergillus species, help identify new targets for antifungal drugs, and enable investigations into the basic biology of fungi. Numerous secondary metabolic pathways with biotechnological applications and pharmacological properties are found in the Aspergilli and the genome sequence will facilitate research in this area.


Trends in Biotechnology | 1998

The optimization of penicillin biosynthesis in fungi

Miguel A. Peñalva; Robert T. Rowlands; Geoffrey Turner

Penicillin production by Penicillium chrysogenum is not only commercially important but arguably the most intensively investigated secondary-metabolic pathway in fungi. Isolation of the structural genes encoding the three main penicillin-biosynthetic enzymes has stimulated the use of molecular approaches to optimize yield and permitted genetic analysis of current production strains, which are themselves the products of 50 years of strain and process improvement. Parallel studies on the penicillin-producing genetic model organism Aspergillus nidulans are now addressing questions about the genetic regulation of primary and secondary metabolism, the compartmentalization of biosynthesis and the excretion of the end products.


Fungal Genetics and Biology | 2003

The Aspergillus nidulans alcA promoter drives tightly regulated conditional gene expression in Aspergillus fumigatus permitting validation of essential genes in this human pathogen

Beatriz Romero; Geoffrey Turner; Israel Olivas; Fernando Laborda; J. Ramón De Lucas

Aspergillus fumigatus causes invasive aspergillosis, a mycosis that is usually fatal in immunocompromised patients. Functional genomics in this fungus will aid the discovery of novel antifungal drugs to treat invasive aspergillosis. However, there is still a need for appropriate molecular genetic tools to facilitate such functional studies. Here, we describe the use of a conditional gene expression system allowing the identification of novel therapeutic targets through validation of essential genes in A. fumigatus. This system is based on the capacity of the Aspergillus nidulans alcA promoter (alcA(p)) to tightly regulate gene expression in this fungus. Conditionally regulated gene expression in A. fumigatus was demonstrated by transcriptional and phenotypic analyses of strains expressing a nuclear migration gene with a terminal phenotype, the A. fumigatus nudC gene, under control of this promoter. This conditional expression system, the first one described in A. fumigatus, will also be useful for investigating the function of essential genes by altering the threonine/glucose ratio in the growth medium.

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Paul Bowyer

University of Sheffield

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Reinhard Fischer

Karlsruhe Institute of Technology

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Paul S. Dyer

University of Nottingham

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