George F. Schreiner
Harvard University
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Advances in Immunology | 1976
George F. Schreiner; Emil R. Unanue
Publisher Summary This chapter discusses membrane and cytoplasmic changes in B lymphocytes induced by ligand–surface immunoglobulin (Ig) interaction. Surface Ig is the receptor for antigen molecules on B lymphocytes. Surface Ig has been identified on the B-cell surface by using immunocytochemistry or direct biochemical analysis. The number of Ig molecules presumably serving as antigen receptors on the B cell surface is on the average of 105 molecules per B cell. The amount of surface-bound Ig is large comparing to the number of hormone receptors needed to trigger certain cellular responses. One could speculate that many of the surface Ig–antigen interactions result in nonfunctional events; therefore, many receptors are needed. The B cell requires such a high number of receptors to effectively bind antigen molecules of large size and variable epitope densities or a number of successive hits are needed in these cells to reach an effective threshold of stimulation. The distribution of surface Ig and other surface macromolecules has been studied at the ultrastructural level by using various methods. One method was the ultrastructural analysis of regular thin section of cells exposed to antibodies conjugated to a large visible molecule.
Journal of Clinical Investigation | 1981
George F. Schreiner; Jeanne-Marie Kiely; Ramzi S. Cotran; Emil R. Unanue
The existence of a subpopulation of rat glomerular cells bearing Ia determinants has been demonstrated with the aid of techniques for the enzymatic isolation and culture of glomerular cells. The Ia-positive cell is normally resident in the uninflamed glomerulus. It resembles a mononuclear phagocyte and consists of a functionally heterogeneous cell population with the capacity of Fc receptor display and phagocytosis, both in vivo and in vitro. A new technique for labeling these cells in situ in intact glomeruli has indicated that Ia-positive cells make up approximately 2% of the total glomerular cell population. The isolated glomerular cells can take up antigen and stimulate immune lymphocytes in an I-region-restricted interaction. They are strongly stimulatory in an allogeneic primary mixed lymphocyte culture. Characterization of this cell type suggests potential new insights into the pathogenesis of renal allograft rejection and immunologically mediated glomerulonephritis.
Oncogene | 2004
Teru Hideshima; Klaus Podar; Dharminder Chauhan; Kenji Ishitsuka; Constantine S. Mitsiades; Yu-Tzu Tai; Makoto Hamasaki; Noopur Raje; Hiromasa Hideshima; George F. Schreiner; Aaron N. Nguyen; Tony A. Navas; Nikhil C. Munshi; Paul G. Richardson; Linda S. Higgins; Kenneth C. Anderson
Although PS-341 (bortezomib) is a promising agent to improve multiple myeloma (MM) patient outcome, 65% of patients with relapsed and refractory disease do not respond. We have previously shown that heat shock protein (Hsp)27 is upregulated after PS-341 treatment, that overexpression of Hsp27 confers PS-341 resistance, and that inhibition of Hsp27 overcomes PS-341 resistance. Since Hsp27 is a downstream target of p38 mitogen-activated protein kinase (MAPK)/MAPK-mitogen-activated protein kinase-2 (MAPKAPK2), we hypothesized that inhibition of p38 MAPK activity could augment PS-341 cytotoxicity by downregulating Hsp27. Although p38 MAPK inhibitor SCIO-469 (Scios Inc, CA, USA) alone did not induce significant growth inhibition, it blocked baseline and PS-341-triggered phosphorylation of p38 MAPK as well as upregulation of Hsp27, associated with enhanced cytotoxicity in MM.1S cells. Importantly, SCIO-469 enhanced phosphorylation of c-Jun NH2-terminal kinase (JNK) and augmented cleavage of caspase-8 and poly(ADP)-ribose polymerase. Moreover, SCIO-469 downregulated PS-341-induced increases in G2/M-phase cells, associated with downregulation of p21Cip1 expression. Importantly, SCIO-469 treatment augmented cytotoxicity of PS-341 even against PS-341-resistant cell lines and patient MM cells. These studies therefore provide the framework for clinical trials of SCIO-469 to enhance sensitivity and overcome resistance to PS-341, thereby improving patient outcome in MM.
Journal of Clinical Investigation | 1987
James B. Lefkowith; George F. Schreiner
Essential fatty acid (EFA) deficiency exerts a beneficial effect on immune-mediated glomerulonephritis, preventing both the tissue injury and consequent mortality. Because both macrophages and eicosanoids are thought to play pathogenic roles in glomerulonephritis, and because macrophages play an important role in modulating arachidonate metabolism at sites of renal injury, the effects of EFA deficiency on the population of resident glomerular macrophages and on glomerular eicosanoid generation were examined. EFA deficiency led to a striking reduction in the number of resident glomerular macrophages and a corresponding reduction in the number of resident glomerular Ia+ cells. This phenomenon was not strain-specific, was not due to a decrease in circulating monocytes, was not a function of changes in cell surface labeling characteristics, and was not restricted to a specific subset of glomeruli. In addition, EFA deficiency affected other areas of the renal cortex: a comparable depletion of interstitial macrophages and Ia+ cells was also observed. In conjunction with the decrease in glomerular macrophages seen with the deficiency state, a marked decrease in both basal and angiotensin II-stimulated glomerular eicosanoid production was noted. In contrast to angiotensin II, platelet-activating factor-induced eicosanoid production was not significantly affected by the deficiency state. These changes in glomerular eicosanoid production could not be attributed to changes in glomerular cyclooxygenase or reacylation capacity. Dietary (n-6) fatty acid supplementation, but not (n-3) fatty acid supplementation, reversed both the decrease in glomerular macrophages and the diminished eicosanoid metabolism seen with the deficiency state. Understanding the mechanisms behind the changes in the glomerular microenvironment induced by EFA deficiency may provide a basis for elucidating the protective effect of dietary fatty acid manipulation on immune-mediated glomerulonephritis.
Clinical Immunology and Immunopathology | 1976
George F. Schreiner; Emil R. Unanue
Abstract Surface complexes of Ig-anti-Ig (Ig, immunoglobulin) readily redistribute into caps. Local anesthetics (lidocaine and chlorpromazine) disrupt the caps, the complexes disseminating over the entire surface. Removal of the anesthetic then allows the complexes to reform into caps. The effects of the anesthetics on cap disruption were counteracted by an increase in extracellular Ca2+ and were not energy dependent. The inhibition of cap formation by anesthetic is also counteracted by increased extracellular Ca2+.
Archive | 1983
Emil R. Unanue; George F. Schreiner; Ramzi S. Cotran
This chapter summarizes a study on a model of immunological renal injury in which a prominent mononuclear cell component infiltrating the glomerulus has been identified (Schreiner et al., 1978). The evidence indicates that the mononuclear cell component contributes to the glomerular injury. The experimental model is an accelerated form of nephrotoxic serum nephritis (NTN).
Journal of Experimental Medicine | 1978
George F. Schreiner; Ramzi S. Cotran; Victoriano Pardo; Emil R. Unanue
Journal of Experimental Medicine | 1977
George F. Schreiner; Keigi Fujiwara; Thomas D. Pollard; Emil R. Unanue
Journal of Immunology | 1975
George F. Schreiner; Emil R. Unanue
Journal of Experimental Medicine | 1976
George F. Schreiner; Emil R. Unanue