George R. Riviere

Transplantation of tooth buds across a multiple non-H-2 barrier.

SUMMARY Adult BALB/c mice were injected with either antilymphocytic serum (ALS) or antithymocytic serum (ATS) 24 hr prior to the heterotopic transplantation of tooth germs from 7-day-old DBA/2 mice to the mammary fat-pad. These strains are identical at the H-2 histocompatibility locus but differ in at least four minor loci. The grafts were examined histologically 1, 2, and 3 weeks after transplantation and assessed for lymphocytic infiltration, hemorrhage, and necrosis. Fifty-seven percent of those tooth germs transplanted to ATS-treated hosts survived, while all those transplanted in ALS-treated hosts appeared similar to noninjected controls, showing various signs of rejection at each of the experimental periods. The earliest signs of rejection were observed 7 days after transplantation. It is concluded that tooth buds, similar to other tissues, can evoke an immune response in allogeneic hosts even in the absence of strong histoincompatibility.

Serum antibody responses to indigenous oral mucosal antigens and selected laboratory-maintained bacteria in recurrent aphthous ulceration☆

Sera from subjects with recurrent aphthous ulceration (RAU) and control subjects were tested for relative levels of IgM, IgG, and IgA antibodies against eight selected laboratory-maintained bacteria, including Streptococcus sanguis which has been implicated in the etiopathogenesis of RAU. There were no differences in relative serum antibody levels for any isotype against any bacteria between control and RAU groups. RAU subjects with active lesions were then paired with control subjects, and each serum was tested against sedimentable material derived from the oral mucosa of each pair member. The analysis of data indicated that RAU and control subjects had similar levels of serum antibodies to indigenous mucosal antigens, but RAU subjects had significantly less antigenic material than control subjects.

Experimental follicular cysts in mice: A histologic study

Abstract Epithelium-lined cysts formed over the crowns of tooth germs which had been transplanted heterotopically to mammary fat pads. A healthy follicular epithelium was necessary for cyst formation. The stimulus for cyst induction may have been a curtailment of the blood supply, but completion of the cysts was dependent upon establishment of the graft in its new environment with concomitant revascularization. Additional although indirect evidence is presented which further links an unsuccessful eruption process with follicular cyst formation.

Alloantibodies Induced in Histoincompatible Rats by Single Pulp and Tooth Grafts

Pulp and whole teeth were transplanted between two histoincompatible strains of rats. A bimodal distribution of antibodies occurred. Hemagglutination titers stimulated by teeth were greater than those stimulated by pulp. The peaks probably represent IgM and IgG antibodies. Periodontal tissues may be more antigenic than pulp.

Murine Tooth Organ Transplantation after in vitro Culture

Molar tooth organs were transplanted from fetal and neonatal mouse donors as well as from cap-stage molar tooth organs following ten days of in vitro culture. Tooth organs from neonatal donors appeared to develop better than those from fetal donors in syngeneic recipients. Cultured allogeneic molar tooth organs appeared to survive longer than uncultured controls.

Oral mucosal antigen reactivity during exacerbation and remission phases of recurrent aphthous ulceration

Pooled serum from subjects with active recurrent aphthous ulceration (RAU) and from control subjects was tested against indigenous surface mucosal material from subjects with active RAU, remission RAU subjects, and normal subjects. IgM antibody reactivity in both sera was significantly higher to material from remission RAU subject mucosa than to material from control subjects or subjects with active RAU. IgG antibody activity in both sera was significantly lower to material from active RAU subject mucosa than to material from control subjects or subjects whose RAU was in remission. These results suggest that the character and amount of antigen on mucosa may differ during the pathogenesis of RAU.

Latex Spheres as Immunologic Markers to Demonstrate the Binding of Human Salivary Immunoglobulins to Streptococcus mutans

A scanning electron microscopy technique is described that uses latex beads as immunologic markers for detecting the interaction of salivary antibody with Streptococcus mutans. A Labeling Index (beads per bacterial cell) demonstrated that human salivary antibodies bound at a significantly greater degree to S mutans than to non oral bacteria.

Influence of Serum on Cultured Murine Tooth Organ Allotransplants

Previous investigations indicated that in vitro culture of fetal tooth germs before allotransplantation prolonged graft survival. This study assessed the influence of different sources of serum in culture medium on post-transplant development of neonatal tooth organs. The results indicate that recipient-strain serum, but not donor-strain serum or calf serum, increased tooth survival compared with that of uncultured controls, which were rejected by the fourteenth post-transplant day. The survival curve for cultured tooth organs was shifted to the right by two days. No tooth transplant survived beyond three wk.

Rejection of Skin Grafts After Orthotopic Tooth Transplantation Between RhLA-paired Monkeys

Statistical analysis of paired observations indicated that RhLA-A,B disparate teeth caused the accelerated rejection of tooth-donor skin grafts placed 21 to 24 mo after tooth allotransplantation, but not at 30 to 34 mo. Teeth transplanted between matched monkeys did not cause the accelerated rejection of skin grafts.

Antibodies to Tooth Allografts in H-2 Matched Mice

Teeth were transplanted among mice which represented the best match possible between unrelated humans. Sera were examined for their ability to kill donor cells with the help of complement, to trigger complement-independent, cell-mediated cytolysis, and to agglutinate RBC. All responses were weak, but rejection correlated best with antibody-dependent cytolysis.