Georges Debrégeas

The Role of Fingerprints in the Coding of Tactile Information Probed with a Biomimetic Sensor

In humans, the tactile perception of fine textures (spatial scale <200 micrometers) is mediated by skin vibrations generated as the finger scans the surface. To establish the relationship between texture characteristics and subcutaneous vibrations, a biomimetic tactile sensor has been designed whose dimensions match those of the fingertip. When the sensor surface is patterned with parallel ridges mimicking the fingerprints, the spectrum of vibrations elicited by randomly textured substrates is dominated by one frequency set by the ratio of the scanning speed to the interridge distance. For human touch, this frequency falls within the optimal range of sensitivity of Pacinian afferents, which mediate the coding of fine textures. Thus, fingerprints may perform spectral selection and amplification of tactile information that facilitate its processing by specific mechanoreceptors.

Whole-brain functional imaging with two-photon light-sheet microscopy

To the Editor: Several studies recently demonstrated that one-photon (1P) light-sheet imaging gives access to the spontaneous activity of a large fraction of the zebrafish larval brain at nearly single-cell resolution1–3. This imaging method, however, requires an intense and extended illumination at a wavelength (l = 488 nm) that lies within the most sensitive region of the fish visible spectrum4 and therefore likely stimulates the blue photoreceptors in the retina as well as other photosensitive cells5. As an alternative, we report on brain-wide three-dimensional (3D) neural recordings during visuomotor integration in zebrafish larvae using two-photon (2P) light-sheet imaging6 at a wavelength of 930 nm combined with visual stimulation. The extent to which 1P illumination interferes with visually driven processes is critical as it determines the suitability of light-sheet imag-

Effect of fingerprints orientation on skin vibrations during tactile exploration of textured surfaces.

In humans, the tactile perception of fine textures is mediated by skin vibrations when scanning the surface with the fingertip. These vibrations are encoded by specific mechanoreceptors, Pacinian corpuscules (PCs), located about 2mm below the skin surface. In a recent article, we performed experiments using a biomimetic sensor which suggest that fingerprints (epidermal ridges) may play an important role in shaping the subcutaneous stress vibrations in a way which facilitates their processing by the PC channel. Here we further test this hypothesis by directly recording the modulations of the fingerpad/substrate friction force induced by scanning an actual fingertip across a textured surface. When the fingerprints are oriented perpendicular to the scanning direction, the spectrum of these modulations shows a pronounced maximum around the frequency v/λ, where v is the scanning velocity and λ the fingerprints period. This simple biomechanical result confirms the relevance of our previous finding for human touch.

Whisker encoding of mechanical events during active tactile exploration

Rats use their whiskers to extract a wealth of information about their immediate environment, such as the shape, position or texture of an object. The information is conveyed to mechanoreceptors located within the whisker follicle in the form of a sequence of whisker deflections induced by the whisker/object contact interaction. How the whiskers filter and shape the mechanical information and effectively participate in the coding of tactile features remains an open question to date. In the present article, a biomechanical model was developed that provides predictions of the whisker dynamics during active tactile exploration, amenable to quantitative experimental comparison. This model is based on a decomposition of the whisker profile into a slow, quasi-static sequence and rapid resonant small-scale vibrations. It was applied to the typical situation of a rat actively whisking across a solid object. Having derived the quasi-static sequence of whisker deformation, the resonant properties of the whisker were analyzed, taking into account the boundary conditions imposed by the whisker/surface contact. We then focused on two elementary mechanical events that are expected to trigger significant neural responses, namely (1) the whisker/object first contact and (2) the whisker detachment from the object. Both events were found to trigger a deflection wave propagating upward to the mystacial pad at constant velocity of ≈3–5 m/s. This yielded a characteristic mechanical signature at the whisker base, in the form of a large peak of negative curvature occurring ≈4 ms after the event has been triggered. The dependence in amplitude and lag of this mechanical signal with the main contextual parameters (such as radial or angular distance) was investigated. The model was validated experimentally by comparing its predictions to high-speed video recordings of shock-induced whisker deflections performed on anesthetized rats. The consequences of these results on possible tactile encoding schemes are briefly discussed.

Probing the micromechanics of a multi-contact interface at the onset of frictional sliding

Digital Image Correlation is used to study the micromechanics of a multi-contact interface formed between a rough elastomer and a smooth glass surface. The in-plane elastomer deformation is monitored during the incipient sliding regime, i.e. the transition between static and sliding contact. As the shear load is increased, an annular slip region, in coexistence with a central stick region, is found to progressively invade the contact. From the interfacial displacement field, the tangential stress field can be further computed using a numerical inversion procedure. These local mechanical measurements are found to be correctly captured by Cattaneo and Mindlin (CM)s model. However, close comparison reveals significant discrepancies in both the displacement and stress fields that reflect the oversimplifying hypothesis underlying CMs scenario. In particular, our optical measurements allow us to exhibit an elasto-plastic-like friction constitutive equation that differs from the rigid-plastic behavior assumed in CMs model. This local constitutive law, which involves a roughness-related length scale, is consistent with the model of Bureau et al. (Proc. R. Soc. London, Ser. A 459, 2787 (2003)) derived for homogeneously loaded macroscopic multi-contact interfaces, thus extending its validity to mesoscopic scales.Graphical abstract

Stress Field at a Sliding Frictional Contact: Experiments and Calculations

Abstract A MEMS-based sensing device is used to measure the normal and tangential stress fields at the base of a rough elastomer film in contact with a smooth glass cylinder in steady sliding. This geometry allows for a direct comparison between the stress profiles measured along the sliding direction and the predictions of an original exact bidimensional model of friction. The latter assumes Amontons’ friction law, which implies that in steady sliding the interfacial tangential stress is equal to the normal stress times a pressure-independent dynamic friction coefficient μ d , but makes no further assumption on the normal stress field. Discrepancy between the measured and calculated profiles is less than 14% over the range of loads explored. Comparison with a test model, based on the classical assumption that the normal stress field is unchanged upon tangential loading, shows that the exact model better reproduces the experimental profiles at high loads. However, significant deviations remain that are not accounted for by either calculations. In that regard, the relevance of two other assumptions made in the calculations, namely (i) the smoothness of the interface and (ii) the pressure-independence of μ d is briefly discussed.

Contact Dynamics in a Gently Vibrated Granular Pile

We use multispeckle diffusive wave spectroscopy to probe the micron-scale dynamics of a water-saturated granular pile submitted to discrete gentle taps. The typical time scale between plastic events is found to increase dramatically with the number of applied taps. Furthermore, this microscopic dynamics weakly depends on the solid fraction of the sample. This process is largely analogous to the aging phenomenon observed in thermal glassy systems. We propose a heuristic model where this slowing-down mechanism is associated with a slow evolution of the distribution of the contact forces between particles. This model accounts for the main features of the observed dynamics.

Quasi-static rheology of foams. Part 2. Continuous shear flow

The evolution of a bidimensional foam submitted to continuous quasi-static shearing is investigated both experimentally and numerically. We extract, from the images of the sheared foam, the plastic flow profiles as well as the local statistical properties of the stress field. When the imposed strain becomes larger than the yield strain, the plastic events develop large spatial and temporal correlations, and the plastic flow becomes confined to a narrow shear band. This transition and the steady-state regime of flow are investigated by first focusing on the elastic deformation produced by an elementary plastic event. This allows us to understand (i) the appearance of long-lived spatial heterogeneities of the stress field, which we believe are at the origin of the shear-banding transition, and (ii) the statistics of the dynamic fluctuations of the stress field induced by plastic rearrangements in the steady-state regime. Movies are available with the online version of the paper.

A microfluidic device to study neuronal and motor responses to acute chemical stimuli in zebrafish

Zebrafish larva is a unique model for whole-brain functional imaging and to study sensory-motor integration in the vertebrate brain. To take full advantage of this system, one needs to design sensory environments that can mimic the complex spatiotemporal stimulus patterns experienced by the animal in natural conditions. We report on a novel open-ended microfluidic device that delivers pulses of chemical stimuli to agarose-restrained larvae with near-millisecond switching rate and unprecedented spatial and concentration accuracy and reproducibility. In combination with two-photon calcium imaging and recordings of tail movements, we found that stimuli of opposite hedonic values induced different circuit activity patterns. Moreover, by precisely controlling the duration of the stimulus (50–500 ms), we found that the probability of generating a gustatory-induced behavior is encoded by the number of neurons activated. This device may open new ways to dissect the neural-circuit principles underlying chemosensory perception.

Rheotaxis of Larval Zebrafish: Behavioral Study of a Multi-Sensory Process.

Awake animals unceasingly perceive sensory inputs with great variability of nature and intensity, and understanding how the nervous system manages this continuous flow of diverse information to get a coherent representation of the environment is arguably a central question in systems neuroscience. Rheotaxis, the ability shared by most aquatic species to orient toward a current and swim to hold position, is an innate and robust multi-sensory behavior that is known to involve the lateral line and visual systems. To facilitate the neuroethological study of rheotaxic behavior in larval zebrafish we developed an assay for freely swimming larvae that allows for high experimental throughtput, large statistic and a fine description of the behavior. We show that there exist a clear transition from exploration to counterflow swim, and by changing the sensory modalities accessible to the fishes (visual only, lateral line only or both) and comparing the swim patterns at different ages we were able to detect and characterize two different mechanisms for position holding, one mediated by the lateral line and one mediated by the visual system. We also found that when both sensory modalities are accessible the visual system overshadows the lateral line, suggesting that at the larval stage the sensory inputs are not merged to finely tune the behavior but that redundant information pathways may be used as functional fallbacks.