Georges Delvaux

Correlation between beta cell mass and glycemic control in type 1 diabetic recipients of islet cell graft.

Islet grafts can induce insulin independence in type 1 diabetic patients, but their function is variable with only 10% insulin indepence after 5 years. We investigated whether cultured grafts with defined β cell number help standardize metabolic outcome. Nonuremic C-peptide-negative patients received an intraportal graft with 0.5–5.0 × 106 β cells per kilogram of body weight (kgBW) under antithymocyte globulin and mycophenolate mofetil plus tacrolimus. Metabolic outcome at posttransplant (PT) month 2 was used to decide on a second graft under maintenance mycophenolate mofetil/tacrolimus. Graft function was defined by C-peptide >0.5 ng/ml and reduced insulin needs, metabolic control by reductions in HbA1c, glycemia coefficient of variation, and hypoglycemia. At PT month 2, graft function was present in 16 of 17 recipients of >2 × 106 β cells per kgBW versus 0 of 5 with lower number. The nine patients with C-peptide >1 ng/ml and glycemia coefficient of variation of <25% did not receive a second graft; five of them were insulin-independent until PT month 12. The 12 others received a second implant; it achieved insulin-independence at PT month 12 when the first and second graft contained >2 × 106 β cells per kgBW. Of the 20 recipients of at least one graft with >2 × 106 β cells per kgBW, 17 maintained graft function and metabolic control up to PT month 12. At PT month 12, β cell function in insulin-independent patients ranged around 25% of age-matched control values. Thus, 1-year metabolic control can be reproducibly achieved and standardized by cultured islet cell grafts with defined β cell number.

Magnetic Resonance Cholangiopancreatography in the Preoperative Assessment of Patients with Biliary Pancreatitis

Background: The ultimate treatment of acute biliary pancreatitis (ABP) is undoubtedly laparoscopic cholecystectomy, but controversy remains about the optimal imaging method in the preoperative assessment of these patients. In this study, we evaluated the usefulness of magnetic resonance cholangiopancreatography (MRCP) in detecting common bile duct (CBD) stones and associated pathologies in patients with ABP. At the same time, we tried to determine the natural transit time of gallstones from gallbladder to duodenum in ABP. Methods: Between February 1999 and October 2006 a prospective observational study was conducted and 104 consecutive patients with ABP were recruited. MRCP findings were correlated with subsequent endoscopic retrograde cholangiopancreatography, endoscopic ultrasonography, intraoperative cholangiography or clinical follow-up. Results: MRCP correctly predicted the presence of CBD stones in 19 out of 104 patients, and there were two false-positive and four false-negative results. The ability of MRCP to detect CBD stones was: positive predictive value 90.5%, negative predictive value 95.2%, sensitivity 82.6%, specificity 97.5% and overall accuracy 94.2%. MRCP performed within 48 h after admission showed CBD stones in 28.6% of the patients decreasing to 8.0% after 1 week. MRCP disclosed cholecystitis in 25 patients, anatomical variants of the cystic duct in 10 patients and a wide variety of other abnormalities of the upper abdominal cavity. Conclusion: MRCP is highly accurate in the preoperative detection of CBD stones and other biliopancreatic pathologies in patients with gallstone pancreatitis.

Neuroendocrine tumors of the pancreas

Background: The benefits of associating magnetic resonance imaging (MRI) and laparoscopy for localizing and treating neuroendocrine pancreatic tumors (NEPTs) have been poorly documented. Methods: In a retrospective study, eight patients with a mean age of 48 years were operated on for a NEPT. MRI was used to localize the lesions. In all patients a laparoscopic resection was carried out. Laparoscopic ultrasonography (LUS) was used during most operations. Results: The tumor was clearly localized by MRI in seven patients, and LUS showed the lesion in another patient whose preoperative MRI had been unsuitable. Three enucleations, three spleen-preserving caudal pancreatectomies, and two caudal pancreatectomies with splenectomy were carried out. There were no conversions and the mean operating time was 260 min, with a mean blood loss of 180 ml. The mean hospital stay was 7 days. There were no minor nor major complications during the hospitalization period. However, a pancreatic abscess was diagnosed in one patient 1 month later, requiring an urgent laparotomy. Conclusion: MRI as well as LUS are indeed suitable techniques to localize NEPTs. Moreover, the minimally invasive approach ensured an adequate treatment with a more comfortable and short postoperative recovery.

Laparoscopic repair of diaphragmatic Morgagni hernia.

A novel case of laparoscopic repair of a diaphragmatic hernia through the foramen of Morgagni in a 5-year-old boy is reported. The patient had a prompt and complete recovery, with no evidence of recurrence noted at 1 year after surgery. Laparoscopic repair is considered to be a suitable and safe procedure for the treatment of Morgagni hernia.

Postprandial stimulation of epithelial cell proliferation in defunctioned colon of rats is not caused by gastrin

Rats were submitted, at random, to either a diverting colostomy alone or to antrectomy with a colostomy. After a 48-h fasting period, animals from each group were refed, whereas control animals were kept fasting. Animals were killed at 0, 6, 12, 18, and 24 h after the time of refeeding. In vitro labeling of colon mucosa with [3H]thymidine and autoradiography were performed to determine the proliferative parameters in the colonic crypts, and scintillation counts on mucosal scrapings were used for the estimation of mucosal deoxyribonucleic acid synthetic activity. Refeeding increased the labeling index (p less than 0.01), mitotic index (p less than 0.01), and mucosal deoxyribonucleic acid synthesis activity (p less than 0.01) in the proximal colon as well as in the defunctioned distal segment. Despite suppression of the postprandial rise in serum gastrin (p less than 0.01), antrectomy did not abolish the proliferative reaction in any colonic segment. These data confirm the existence of a potent stimulant of colonic cell proliferation that is released systematically after feeding. They indicate that gastrin is not the responsible stimulant and that another, yet unknown, physiological factor is involved.

Laparoscopic wedge resection for gastric ectopic pancreas

Ectopic pancreas is pancreatic tissue found outside the usual anatomic location of the pancreas. It is often an incidental finding in clinical practice and can be found at different sites in the gastrointestinal tract. Although usually a silent anomaly, it may become clinically evident when complicated by pathologic changes such as inflammation, bleeding, obstruction, and malignant transformation. We describe a case of ectopic pancreas located in the stomach, treated with a laparoscopic approach that permitted isolation and complete resection of the lesion. The patient was discharged without complications and without recurrence of symptoms.

Endovascular Techniques in the Management of Acute Arterioenteric Fistulas

PURPOSE To report the use of endovascular techniques to emergently treat hemorrhagic complications of rare arterioenteric fistulas. CASE REPORTS Two patients, a 71-year-old man and a 61-year-old woman, presented with acute bleeding arising from primary arterioenteric fistulas. In the first patient, a fistula between the iliac artery and the small intestine complicated laparoscopic treatment of acute appendicitis. In the second patient, irradiation of a metastatic cervical carcinoma led to a fistula between the right iliac artery and the terminal ileum. In both patients, the hemorrhage was controlled with implantation of a Jostent Peripheral Stent-Graft. The man is alive at 3 years with a patent endograft, but the woman died 1 month after treatment from complications of tumor progression. CONCLUSIONS Endovascular application of covered stents provides an alternative treatment, avoiding extensive surgery. In cases of neoplastic erosion of a large vessel, endovascular stenting can offer a palliative solution.

Isolated Traumatic Gallbladder Rupture

Abstract Blunt traumatic injury to the extrahepatic biliary system is a rare event usually recognized on evaluation and treatment of other visceral injuries during laparotomy. Isolated gallbladder rupture secondary to blunt abdominal trauma is even more uncommon, but poses a potential life-threatening surgical emergency. Delay in the diagnosis of the injury for several days due to no or vague symptoms and an insidious course are common. Early diagnosis is essential, as protracted treatment may result in significant morbidity and mortality. We report the case of a patient who suffered an isolated gallbladder rupture due to blunt abdominal trauma from a fall. The subject of isolated traumatic gallbladder rupture is reviewed because of the rarity of this condition and the diagnostic challenges it poses.

Surgical management of Gastrointestinal Stromal Tumours: A Single Centre Experience During the Past 17 Years

Abstract Background: Gastrointestinal stromal tumour (GIST) is a rare tumour that can arise anywhere within the gastrointestinal tract. Objectives: Our objective was to present our experience managing this rare tumour of the gastrointestinal tract. We reviewed the clinico-pathological and morphological features, our experience with surgical treatment, and the outcome GIST in our centre. Patients and methods: The current retrospective analysis included 64 patients with GIST observed between February 1995 and September 2012. Results: There were 39 males and 25 females. The mean age was 63.2 (range 36–83). The GISTs were located in the stomach in the majority of patients (60 patients, 94.0%). The tumour was asymptomatic in 14 (21.9%) patients. The tumour size varied from 0.4 to 25 cm with a mean size of 7.9 cm. Five patients showed peritoneal or liver metastasis at diagnosis. All patients had surgery. Five patients had a R2 resection and in one patient the resection-free margin was uncertain. In our cohort we had 5 patients with metastasis at diagnosis who received adjuvant imatinib. Four patients developed metastasis in the follow-up period. Three patients died due to GIST, three other patients died due to other disease. Conclusions: Gastric GIST were more common than GIST at other locations. Surgical treatment was the main therapeutic option. Tyosine kinase receptor inhibitors was used as a first line treatment in patients with metastatic GISTs or in patients with recurrence of the disease.

Dysphagia after Colon Interposition Graft for Esophageal Carcinoma

Colon interposition is an established technique for esophageal reconstruction. We describe the case of primary adenocarcinoma arising in a colonic interposition graft that was performed after total esophagectomy for recurrence adenocarcinoma derived from the Barrett esophagus.