Georges Lejeune

Sciatic Nerve Regeneration through Venous or Nervous Grafts in the Rat

This study analyses the interest of isologous venous grafts filled with saline or with Schwann cells versus nerve grafts as guides for regeneration of the sciatic nerve in 35 Wistar rats. Electrophysiological parameters (conduction velocities and distal latencies of motor responses) and the functional index of De Medinacelli were measured several times from 1 month to 1 year after surgery. An histological analysis was performed on 2 control rats and on 3 rats killed 6 or 12 months after surgery: the total number of fibers was counted on a montage photoprint of the whole nerve, and the diameters of axons and the thickness of the myelin sheath were measured on digitized images. With a portion of nerve as guide, the regeneration is faster than with a vein. However, regeneration after 6 months is at least as good with a venous graft filled with Schwann cells, as assessed by electrophysiological, functional, and histological analysis. The addition of Schwann cells in grafted veins allows the nerve to regenerate through longer gaps than previously described (25 vs 15 mm). In order to assess the quality of nerve regeneration, functional, electrophysiological, and histological analysis are complementary.

Non-toxic antiseptic irrigation with chlorhexidine in experimental revascularization in the rat.

The effect of different wound irrigation fluids upon femoral arteries and veins was investigated in the rat, using microsurgical techniques. Toxicity was evaluated by microscopical observation after selective staining of histological slides. Povidone-iodine, 10%, proved to be a very irritant solution, provoking an attack on the vascular endothelium and secondary thrombosis. Chlorhexidine at 0.05%, 0.02% and 0.001% was found by contrast to have a very low toxicity which was comparable to physiological saline. Experimental investigation of antiseptic solutions should not only include the determination of the antibacterial effect, but also the potential for cell toxicity, using an irrigation technique.

History of Solid Organ Transplantation at the University of Liège

Doctor Georges Lejeune, who was just graduated MD and was working in the laboratory of Pr Albert, read in 1953 the paper published by Pr P. B. Medawar in Nature, and entitled : “Actively acquired tolerance to foreign cells”. Following this paper, regular contacts were conducted with Pr P. B. Medawar, and in 1959 an International Symposium on “Biological problems of grafting” was organised in Liège under the presidence of Pr P. B. Medawar (Fig. 1). Most participants were well known, as for example Pr Owen, Pr Simonsen, Pr Trentin and Pr Voisin. The proceedings of this symposiHistory of Solid Organ Transplantation at the University of Liège