Georgia Brellou

Outbreak of Avian Mycobacteriosis in a Flock of Two-Year-Old Domestic Pigeons (Columba livia f. domestica)

Abstract This report describes an outbreak of avian mycobacteriosis in a flock of 100 two-yr-old pigeons. Over a 6-mo period, the sick pigeons showed cachexia followed by death. In Columbiformes classic tubercles rarely develop, but in these affected pigeons granulomatous nodular lesions of various sizes, containing numerous acid-fast bacilli, were found in the internal organs. The lesions were observed in the liver, spleen, intestine, bone marrow, ovary, and oviduct. Despite their breeding age, atrophy was also found in the ovary and oviduct. Microorganisms belonging to Mycobacterium avium complex were identified in the affected tissues by polymerase chain reaction.

Inhibition of MCP-1 and MIP-2 chemokines in murine trichinellosis: effect of the anti-inflammatory compound L-mimosine.

Mimosine, is a plant amino-acid which has been reported to block DNA replication in mammalian cells and to arrest cells reversibly towards the end of the G1 phase or at the beginning of the S phase. In this study, 42 mice were infected with T. spiralis, a nematode parasite, and treated with the anti-inflammatory compound L-mimosine, to determine if any alteration in the chronic inflammatory state occurred, by investigating the hosts immunological response. MCP-1, a C-C chemokine and MIP-2, a C-X-C chemokine were tested and measured in the sera of infected animals, after 1, 10, 20, 30, 40, 50 and 60 days postinfection, by ELISA method. The diaphragm/muscle and the masseters of the infected mice, were tested for inflammatory response. We found that MCP-1 was partially inhibited by L-mimosine, while MIP-2 was totally inhibited. Moreover, in sections of the diaphragm and masseters, the infiltration of inflammatory cells such as macrophages, lymphocytes and eosinophils were more intense in untreated animals compared to those treated with L-mimosine. These findings show, that L-mimosine may have an inhibitory effect on MCP-1 and MIP-2 serum levels in Trichinellosis and may influence the recruitment of inflammatory cells and the intensity of the inflammatory reaction in this parasitic disease.

Acute coenurosis of dairy sheep from 11 flocks in Greece

Abstract CASE HISTORY: A syndrome of acute neurological dysfunction with increased mortality was observed in lambs of 10 dairy sheep flocks and adult animals in one flock in Central and Northern Greece. Each farmer completed a questionnaire regarding the management and feeding of their flocks. In seven of the 11 flocks the affected animals were grazing pasture, while in the remaining four flocks (5, 8, 9, 10) the animals were fed alfalfa hay (Medicago sativa) and concentrates indoors. A follow-up study of the affected flocks was conducted during the next 12 months. CLINICAL FINDINGS: Of 42 sheep with acute coenurosis that were examined, the most prominent neurological abnormalities were ataxia, depression, blindness, scoliosis, coma and dysmetria. Except for the four sheep that were comatose, all other animals had normal body temperatures and their appetites remained normal or were slightly decreased. Haematological findings of 15 examined sheep were within normal limits. The affected sheep were subject to euthanasia. A histopathological examination was performed in 13 cases. Faecal samples from dogs associated with these flocks were negative for taeniid infections. During the following 12 months cases of chronic coenurosis in these flocks were observed. PATHOLOGICAL FINDINGS: In the 42 animals that were necropsied, the main gross findings were cystic formations between 0.5–1 cm in diameter with translucent walls that were seen lying free on the leptomeninges or partly penetrating the brain tissue, sterile microabscecess and brain necrosis. Histopathological evaluation of tissue sections of 13 brains showed multifocal purulent or pyogranulomatous meningoencephalitis, accompanied by eosinophilic infiltrations. No bacteria were isolated following bacterial culture of brain tissue Parasitological examination of the cysts from five cases revealed whitish specks on the transparent cyst wall and germination membrane representing the scolices. DIAGNOSIS: Acute coenurosis was diagnosed in all cases studied. CLINICAL RELEVANCE: Acute coenurosis can be one of the causes of acute encephalopathy mainly in lambs, but also in adult sheep. This condition is incurable, but can be controlled by changing the feeding regime. Cases of chronic coenurosis may be seen a few months later in the same flock.

Serum and liver iron concentration in dogs with experimentally induced hepatopathy

Background and Aim:  Iron (Fe) status is altered in human and experimental animal hepatopathies. In dogs limited data are available. The aim of this study was to investigate serum iron (SI), total iron binding capacity (TIBC), percentage transferrin saturation (SAT) and Fe status in the liver of dogs with experimentally induced hepatopathy.

A Clinical Case of Chicken Infectious Anemia Disease and Virus DNA Detection in Naturally Infected Broilers in Greece

Abstract In this study, chicken infectious anemia virus (CIAV) DNA was detected from 12-day-old broilers. Clinical history showed that the clinical features were diarrhea, blue wing disease, depression, and death. Necropsy findings were pale liver, severe atrophy of bursa of Fabricius and thymus, and discoloration of the bone marrow as well as hemorrhages subcutaneously and a few in skeletal muscles. The majority of the necropsied broilers had developed gangrenous dermatitis. Histopathology showed hypoplasia of bone marrow and depletion of lymphocytes in spleen, bursa, and subcapsular thymic cortex. Karyorrhexis of lymphocytes was scattered in the thymic cortex and most pronounced in the bursal follicles. Eosinophilic intranuclear inclusion bodies were mainly located in lymphocytes of thymus, with a few in hemopoietic cells of bone marrow. CIAV DNA was detected by polymerase chain reaction from bursa, thymus, and bone marrow. A virus strain was detected and genetically characterized in 639 base pairs of VP1 gene. Phylogenetic analysis revealed that the Greek isolate was clustered together with isolates from Alabama, China, Slovenia, and Bangladesh.

Surgical management of recurrent cervical sialoceles in four dogs.

Recurrent cervical sialoceles were diagnosed in four dogs associated with inadequate excision of the sublingual gland. Three dogs were managed by resection of the remnants of the sublingual gland via an oral approach. One dog was managed through a ventral approach as identification of the sublingual gland was not possible with the oral approach. The outcome was favourable in all cases and no relapses were detected after a median follow-up time of 10 months.

Clinical and laboratory investigation of experimental acute pancreatitis in the cat

The main objective of this study is the serial evaluation of clinical and laboratory changes in cats with experimentally-induced acute pancreatitis (AP). A total of 16 clinically healthy young adult DSH cats were included in the study. Acute pancreatitis was induced in 10 (AP group) by infusing oleic acid into the pancreatic duct, while the other 6 cats served as sham-operated controls (C group). Depression or lethargy, anorexia, abdominal pain, and a palpable mass in the anterior abdomen were the main clinical findings in cats of the AP group. Laboratory data revealed non-regenerative anaemia, mild hypoalbuminaemia, transient hyperglycaemia, and hypertriglyceridaemia. Serum lipase activity, feline trypsin-like immunoreactivity (fTLI) concentration, and feline pancreatic lipase immunoreactivity (fPLI) concentration increased significantly within the first 24 hours after the infusion of oleic acid, with time-dependent positive correlation. In both group of cats urine amylase-creatinine ratio and amylase-creatinine clearance ratio were increased. In cats of the AP group, abdominal ultrasonography revealed a hypoechogenic and enlarged pancreas, along with peripancreatic fluid accumulation. Peritoneal effusion was consistent with a sterile exudate, with higher lipase but lower amylase activities when compared to their serum counterparts in all AP group cats. The induction of AP was confirmed by pancreatic histopathology. AP is characterized by vague symptomatology. Increases in serum fTLI and fPLI concentrations occur early in cats with pancreatitis. In general the two analytes run a parallel course, but fPLI may be increased longer than fTLI. The measurement of lipase activity in the peritoneal fluid could be considered diagnostic.

Multidetector computed tomographic pulmonary angiography in a cat with fatal heartworm disease

A 17-month-old neutered male domestic shorthair cat was referred for a computed tomographic (CT) study of the thorax due to respiratory distress. Multidetector CT angiography showed a multifocal interstitial ground glass opacity, tortuous and blunted pulmonary arteries consistent with thromboembolism with perivascular lung infiltration and hypoventilation in multiple lung lobes. A blood antigen test was positive for Dirofilaria immitis. The cats clinical condition rapidly declined and the owners elected euthanasia. The histopathologic examination confirmed heartworm disease with parasitic pulmonary thromboembolism.

Prevalence of increased canine pancreas- specific lipase concentrations in young dogs with parvovirus enteritis

BACKGROUND Pancreatic abnormalities during canine parvovirus (CPV) enteritis have not been studied prospectively. OBJECTIVES The objective of this study was to evaluate the diagnostic significance of canine serum pancreas-specific lipase (Spec cPL) concentration in dogs with CPV enteritis for the presence of acute pancreatitis (AP). Puppies with naturally occurring CPV enteritis were recruited and prospectively allocated into 2 groups according to normal or increased serum Spec cPL concentration. Clinical signs, laboratory findings, and pancreas-associated variables were compared between groups, and the impact of possible AP on disease course, duration of hospitalization, and outcome was assessed. RESULTS Serum Spec cPL concentration in 35 puppies was above the upper limit of the RI in 17/35 (48.6%) dogs (Group A) and within the RI in 18 dogs (Group B). An increased serum lipase activity was present in 29/35 (82.9%) dogs, and Group A dogs had a higher serum lipase activity than Group B (P = .006). Serum Spec cPL in Group A dogs was positively correlated with serum lipase activity at the day of presentation (r = .667; P = .003) and day of discharge (r = .628; P = .007). No statistically significant difference was found between groups (P = .233) for the presence of systemic inflammatory response syndrome (SIRS) (6/17 or 35.3% dogs Group A, and 8/18 or 44.4% dogs Group B), the disease course, duration of hospitalization, or outcome between groups. CONCLUSIONS Increased serum Spec cPL is relatively common in dogs with CPV enteritis. However, such increases do not seem to correlate with the outcome of disease.