Géraldine Bolen

Impact of successive freezing-thawing cycles on 3-T magnetic resonance images of the digits of isolated equine limbs

OBJECTIVEnTo assess the impact of cycles of freezing and thawing on magnetic resonance (MR) images (obtained by use of a 3-T magnet) of equine feet examined ex vivo.nnnSAMPLEn9 forelimbs from 9 horse cadavers.nnnPROCEDURESn9 forefeet underwent MR imaging first at ambient temperature within 12 hours after the horses death and then after each freezing-thawing cycle. Three digits underwent freezing and thawing (at 4°C for 36 hours) 2 times, 3 digits underwent freezing and thawing (at 4°C for 36 hours) once and rescanning after 24 hours at ambient temperature, and 3 digits underwent freezing and thawing at ambient temperature for 24 hours once. Images of the digits obtained prior to freezing were subjectively compared with images obtained after freezing and thawing. Changes in the signal-to-noise ratio between examinations were assessed.nnnRESULTSnOverall image quality was considered unchanged except for the hoof capsule. Quantitative analysis revealed signal-to-noise ratio changes in bone marrow, soft tissues, and hoof capsule induced with both thawing processes. The signal-to-noise ratio in the synovial recess of the distal interphalangeal joint significantly increased as a result of thawing at 4°C.nnnCONCLUSIONS AND CLINICAL RELEVANCEnAlthough overall image quality was considered unchanged except for the hoof capsule, results suggested that changes induced in cadaver limbs following freezing and thawing, which are probably attributable both to modified and inhomogeneous temperature distribution and direct tissue damage, may alter the reliability of signal intensity in ex vivo MR examinations.

ULTRASONOGRAPHIC CHARACTERISTICS OF THE CISTERNA CHYLI IN EIGHT DOGS AND FOUR CATS

Ultrasonography of the cisterna chyli has been used in humans to diagnose increased lymphatic flow or lymph flow obstruction and to guide percutaneous embolization of the thoracic duct via the cisterna chyli. The aim of this study was to describe the ultrasonographic characteristics of the dorsal portion of cisterna chyli in dogs and cats with chylous ascites or chylothorax and in a group of healthy dogs and cats. The aorta and the cranial mesenteric artery were used as anatomic landmarks. Ultrasonography was performed before and 2 h after a fatty meal in healthy dogs and cats. The visualized structure was confirmed to be a dilated cisterna chyli at necropsy in a dog with chylous ascites. The confirmed or presumed cisterna chyli was consistently detected using ultrasonography in nonfasted healthy animals and clinically affected animals and appeared as an anechoic tubular structure, without detectable flow, at the right dorsolateral aspect of the aorta. It had a similar ultrasonographic appearance in patients with chyloabdomen and in nonfasted healthy dogs and cats. There was considerable overlap in diameters of the cisterna chyli for affected and healthy animals. The shape and size of the cisterna chyli in an individual animal were variable during the same ultrasound examination and between different examinations. This study demonstrated the appearance of the presumed dorsal portion of the cisterna chyli by ultrasonography and might provide useful preliminary data for further studies into the feasibility of ultrasound-guided injections or aspirations of the cisterna chyli in dogs and cats.