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Dive into the research topics where Gerardo Botti is active.

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Featured researches published by Gerardo Botti.


American Journal of Transplantation | 2007

Prospective monitoring of polyomavirus BK replication and impact of pre-emptive intervention in pediatric kidney recipients.

Fabrizio Ginevri; Alberta Azzi; Hans H. Hirsch; Sabrina Basso; I. Fontana; M. Cioni; Sohrab Bodaghi; Vittorio Salotti; Alessio Rinieri; Gerardo Botti; Francesco Perfumo; Franco Locatelli; Patrizia Comoli

Polyoma BK virus (BKV)‐associated nephropathy (PVAN) is a relevant cause of poor renal allograft survival. In a prospective analysis, we monitored BKV DNA in blood and urine samples from 62 consecutive pediatric kidney recipients. In patients with BKV replication, we analyzed the impact of reduction of maintenance immunosuppression on viral load kinetics and PVAN in patients with BKV replication. BKV‐specific immunity was concomitantly evaluated on blood samples of viremic patients, by measuring the frequency of BKV‐specific interferon‐γ‐producing and cytotoxic T cells, and BKV IgG antibody levels. At a median follow‐up of 24 months, BK viruria was observed in 39 of 62 patients, while BK viremia developed in 13 patients (21%). In all viremic patients, immunosuppression reduction resulted in the clearance of viremia, and prevented development of PVAN, without increasing the rate of acute rejection or causing graft dysfunction. As a consequence of immunosuppression adjustment, an expansion of BKV‐specific cellular immunity was observed that coincided with viral clearance. We conclude that treating pediatric kidney transplant patients pre‐emptively with immunosuppression reduction guided by BKV DNA in blood is safe and effective to prevent onset of PVAN. BKV‐specific cellular immunity may be useful to guide this intervention.


American Journal of Transplantation | 2005

Treatment of EBV-Related Post-Renal Transplant Lymphoproliferative Disease with a Tailored Regimen Including EBV-Specific T Cells

Patrizia Comoli; Rita Maccario; Franco Locatelli; Umberto Valente; Sabrina Basso; Alberto Garaventa; Paolo Tomà; Gerardo Botti; Giovanni Melioli; Fausto Baldanti; Arcangelo Nocera; Francesco Perfumo; Fabrizio Ginevri

The treatment of EBV‐associated post‐transplant lymphoproliferative disease (PTLD) poses a considerable challenge. Efforts have been made to define regimens based on combination of the available therapeutic agents, chosen and tailored on a patient‐by‐patient basis, with the aim of augmenting event‐free patient and graft survival. Recently, autologous EBV‐specific cytotoxic T‐lymphocytes (CTL) have proved effective in enhancing EBV‐specific immune responses and reducing viral load in organ transplant recipients with active infection. We investigated the use of a tailored combined approach including autologous EBV‐specific CTL for the treatment of EBV‐related PTLD developing after pediatric kidney transplantation.


Transplantation | 2004

Polyomavirus BK-specific immunity after kidney transplantation.

Patrizia Comoli; Alberta Azzi; Rita Maccario; Sabrina Basso; Gerardo Botti; Giancarlo Basile; I. Fontana; Massimo Labirio; Angela Cometa; Francesca Poli; Francesco Perfumo; Franco Locatelli; Fabrizio Ginevri

Failure to mount or maintain a protective immune response may influence the development of polyomavirus BK (BKV)-associated nephropathy (PVAN). However, limited data are so far available on BKV-specific immunity after kidney transplantation. BKV-specific cellular immune response was retrospectively analyzed in kidney recipients with or without BKV infection/reactivation by measuring the frequency of interferon (IFN)-gamma-secreting cells in peripheral blood. Patients with BKV-active infection and good renal function (n=6) had a mean BKV-specific lymphocyte frequency 2 log lower than healthy controls and in the same range as BKV-seropositive recipients without active infection (n=7). Patients with PVAN (n=5) revealed undetectable levels of BKV-specific cells. However, two patients from the latter cohort treated with immunosuppression reduction showed the emergence of specific immunity, with IFN-gamma production in the same range as healthy controls. Our preliminary data suggest that lack of protective immunity toward BKV may favor the occurrence of BKV active infection and influence the progression to PVAN.


International Congress of the Transplantation Society | 2003

Polyomavirus BK infection in pediatric kidney-allograft recipients: A single-center analysis of incidence, risk factors, and novel therapeutic approaches

Fabrizio Ginevri; R. De Santis; Patrizia Comoli; Nadia Pastorino; C. Rossi; Gerardo Botti; I. Fontana; Arcangelo Nocera; Massimo Cardillo; M. R. Ciardi; Franco Locatelli; Rita Maccario; Francesco Perfumo; Alberta Azzi


Nephrology Dialysis Transplantation | 1998

Cell-specific regulation of alpha1(III) and alpha2(V) collagen by TGF-beta1 in tubulointerstitial cell models.

Roberta Bertelli; Federico Valenti; Roberta Oleggini; Gianluca Caridi; Paola Altieri; Domenico A. Coviello; Gerardo Botti; Roberto Ravazzolo; Gian Marco Ghiggeri


Nephrology Dialysis Transplantation | 2000

Characterization of a DNA binding site that mediates the stimulatory effect of cyclosporin‐A on type III collagen expression in renal cells

Roberta Oleggini; Luca Musante; Stefania Menoni; Gerardo Botti; Marco Di Duca; Michela Prudenziati; Alba Carrea; Roberto Ravazzolo; Gian Marco Ghiggeri


Giornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia | 2006

[Polyomavirus BK-associated nephropathy after kidney transplantation].

Fabrizio Ginevri; Azzi A; Gerardo Botti; Patrizia Comoli


Nephrology Dialysis Transplantation | 1998

Cell-specific regulation of α1(III) and α2(V) collagen by TGF-β1 in tubulointerstitial cell models

Roberta Bertelli; Federico Valenti; Roberta Oleggini; Gianluca Caridi; Paola Altieri; Domenico A. Coviello; Gerardo Botti; Roberto Ravazzolo; Gian Marco Ghiggeri


Nephrology Dialysis Transplantation | 1998

Cell-specific regulation of a 1 (III ) and a 2 ( V ) collagen by TGF-b 1 in tubulointerstitial cell models

Roberta Bertelli; Federico Valenti; Roberta Oleggini; Gianluca Caridi; Paola Altieri; Domenico A. Coviello; Gerardo Botti; Roberto Ravazzolo; Gian Marco Ghiggeri


Transplantation | 2004

Role Of Exogenous Polyomavirus Bk (bkv) Strains In The Development Of Active Viral Infection After Retransplantation For Bkv Nephropathy

Fabrizio Ginevri; Patrizia Comoli; R. De Santis; Gerardo Botti; Sabrina Basso; I. Fontana; Francesco Perfumo; Umberto Valente; Alberta Azzi

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