Ghizan Saleh

Genetic variability of oil palm parental genotypes and performance of its' progenies as revealed by molecular markers and quantitative traits.

Studies were conducted to assess the genetic relationships between the parental palms (dura and pisifera) and performance of their progenies based on nine microsatellite markers and 29 quantitative traits. Correlation analyses between genetic distances and hybrids performance were estimated. The coefficients of correlation values of genetic distances with hybrid performance were non-significant, except for mean nut weight and leaf number. However, the correlation coefficient of genetic distances with these characters was low to be used as predicted value. These results indicated that genetic distances based on the microsatellite markers may not be useful for predicting hybrid performance. The genetic distance analysis using UPGMA clustering system generated 5 genetic clusters with coefficient of 1.26 based on quantitative traits of progenies. The genotypes, DP16, DP14, DP4, DP13, DP12, DP15, DP8, DP1 and DP2 belonging to distant clusters and greater genetic distances could be selected for further breeding programs.

An efficient in vitro plantlet regeneration from shoot tip cultures of Curculigo latifolia, a medicinal plant

A procedure was developed for in vitro propagation of Curculigo latifolia through shoot tip culture. Direct regeneration and indirect scalp induction of Curculigo latifolia were obtained from shoot tip grown on MS medium supplemented with different concentrations and combinations of thidiazuron and indole-3-butyric acid. Maximum response for direct regeneration in terms of percentage of explants producing shoot, shoot number, and shoot length was obtained on MS medium supplemented with combination of thidiazuron (0.5 mg L−1) and indole-3-butyric acid (0.25 mg L−1) after both 10 and 14 weeks of cultures. Indole-3-butyric acid in combination with thidiazuron exhibited a synergistic effect on shoot regeneration. The shoot tips were able to induce maximum scalp from basal end of explants on the medium with 2 mg L−1 thidiazuron. Cultures showed that shoot number, shoot length, and scalp size increased significantly after 14 weeks of culture. Transferring of the shoots onto the MS medium devoid of growth regulators resulted in the highest percentage of root induction and longer roots, while medium supplemented with 0.25 mg L−1 IBA produced more numbers of roots.

Genetic Performance and General Combining Ability of Oil Palm Deli dura x AVROS pisifera Tested on Inland Soils

The performance of 11 oil palm AVROS (Algemene Vereniging van Rubberplanters ter Oostkust van Sumatra) pisiferas was evaluated based on their 40 dura x pisifera (DxP) progenies tested on inland soils, predominantly of Serdang Series. Fresh fruit bunch (FFB) yield of each pisiferas ranged from 121.93 to 143.9 kg palm−1 yr−1 with trial mean of 131.62 kg palm−1 yr−1. Analysis of variance (ANOVA) showed low genetic variability among pisifera parents for most of the characters indicating uniformity of the pisifera population. This was anticipated as the AVROS pisiferas were derived from small population and were inbred materials. However, some of the pisiferas have shown good general combining ability (GCA) for certain important economic traits. Three pisiferas (P1 (0.174/247), P3 (0.174/498), P11 (0.182/308)) were identified of having good GCA for FFB yield while pisiferas P1 (0.174/247), P10 (0.182/348), and P11 (0.182/308) were good combiners for oil-to-bunch ratio (O/B). The narrow genetic base of these materials was the main obstacle in breeding and population improvement. However, efforts have been made to introgress this material with the vast oil palm germplasm collections of MPOB for rectifying the problem.

Combining Ability Analysis in Complete Diallel Cross of Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai)

The experiments were carried out in two research stations (MARDI Bukit Tangga, Kedah, and MARDI Seberang Perai, Penang) in Malaysia. The crossings were performed using the four inbred lines in complete diallel cross including selfs and reciprocals. We evaluated the yield components and fruit characters such as fruit yield per plant, vine length, days to fruit maturity, fruit weight, total soluble solid content, and rind thickness over a period of two planting seasons. General combining ability and its interaction with locations were statistically significant for all characteristics except number of fruits per plant across the environments. Results indicated that the additive genetic effects were important to the inheritance of these traits and the expression of additive genes was influenced greatly by environments. In addition, specific combining ability effect was statistically evident for fruit yield per plant, vine length, days to first female flower, and fruit weight. Most of the characters are simultaneously controlled by additive and nonadditive gene effects. This study demonstrated that the highest potential and promising among the crosses was cross P2 (BL-14) × P3 (6372-4), which possessed prolific plants, with early maturity, medium fruit weight and high soluble solid contents. Therefore this hybrid might be utilized for developing high yielding watermelon cultivars and may be recommended for commercial cultivation.

Heritability and gene effects for root characteristics in peas measured at flowering

SummaryHeritability and gene effects for root weight, root volume and root to shoot weight ratio were determined in peas (Pisum sativum L.) at flowering. The populations used were developed from four crosses between lines and cultivars differing in size of the root systems. Broad-sense heritability was between 0.41 and 0.81 for root weight, and between 0.44 and 0.77 for root volume. Additive and dominance effects were important in the genetic control of root weight and volume in all populations, while epistatic effects were important only in two populations. The importance of the genetic parameters in the control of root to shoot weight ratio was unclear. Assuming high correlations between root growth in soil-filled pots in the greenhouse and growth in the field, with the presence of large additive effects and high heritability estimates for root weight and volume, selection for superior pure lines with large root systems should be effective.

Demarcation of informative chromosomes in tropical sweet corn inbred lines using microsatellite DNA markers

A study of genetic variation among 10 pairs of chromosomes extracted from 13 tropical sweet corn inbred lines, using 99 microsatellite markers, revealed a wide range of genetic diversity. Allelic richness and the number of effective alleles per chromosome ranged from 2.78 to 4.33 and 1.96 to 3.47, respectively, with respective mean values of 3.62 and 2.73. According to the Shannon’s information index (I) and Nei’s gene diversity coefficient (Nei), Chromosome 10 was the most informative chromosome (I = 1.311 and Nei = 0.703), while Chromosome 2 possessed the least (I = 0.762 and Nei = 0.456). Based on linkage disequilibrium (LD) measurements for loci less than 50 cM apart on the same chromosome, all loci on Chromosomes 1, 6 and 7 were in equilibrium. Even so, there was a high proportion of genetic variation in Chromosomes 4, 5, 8, 9 and 10, thereby revealing their appropriateness for use in the genetic diversity investigations among tropical sweet corn lines. Chromosome 4, with the highest number of loci in linkage disequilibrium, was considered the best for marker-phenotype association and QTL mapping, followed by Chromosomes 5, 8, 9 and 10.

Corn yield response to crowding stress and cropping season

Corn (Zea mays L.) is planted in two seasons per year in northern Iran (mid-April as a main crop and mid-June as a second crop). The main objective of this study was to determine whether corn yield response would differ between these two seasons and different plant populations. Two field experiments were conducted at the Agricultural Research Center of Golestan – Iran in 2007 and 2008 at different planting densities. The results showed that the values of grain yield and most traits were significantly lower in the second season. Maximum grain yield was observed at planting densities of 6.5 plants m−2 in the first season, whereas in the second season grain yield was the same for planting densities between 2.5 and 12.5 plants m−2. Based on the second-year experimental results, the following functions were fitted to show the relationship between yield ha−1 (Y) and planting densities (X) for the first and second seasons, respectively: (Y = −167.6X 2 + 2672.2X + 511.77; R 2 = 0.992) and (Y = 1200.1 ln(X) + 2924.4; R 2 = 0.935). This study found that the optimum plant population was 6.5 plants m−2 under low heat stress, and should be reduced to 2.5–4.5 plants m−2 under heat stress conditions.

Generation mean analysis of grain quality traits in selected rice populations derived from different amylose characteristics

BACKGROUND Genetic analysis using generation mean analysis is a tool for designing the most appropriate breeding approaches to developing varieties of rice. It estimates the gene actions that control quantitative traits, as well as the additive, dominance and epistatic effects. This study was conducted using three rice populations that were derived from parental lines with different amylose content. The aim was to partition the gene actions using generation mean analysis for the selected populations. RESULTS A scaling test was carried out to evaluate the fulfilment of the additive-dominance model. Non-allelic interaction was observed for milled grain length, length-to-width ratio and milled rice recovery of all populations evaluated. An additive-dominance model was not adequate for amylose, gel consistency, grain length, grain width, milled grain width and head rice recovery, thus epistasis was involved in the populations evaluated. The importance of additive gene action was observed for grain length, milled grain length and milled rice recovery for populations of high- and low-amylose parents. However, populations with intermediate- and high-amylose parents and intermediate- and low-amylose parents shared almost similar dominance gene actions for most of the physical grain quality traits. CONCLUSION These results suggested that delayed selection is the best approach for traits governed by dominance and epistasis effects. Meanwhile, the traits that were governed by additive effects should undergo thorough selection at an early stage.