Ghulam Jilani

Insights into cadmium induced physiological and ultra-structural disorders in Juncus effusus L. and its remediation through exogenous citric acid

This study appraised cadmium (Cd) toxicity stress in wetland plant Juncus effusus, and explored its potential for Cd phytoextraction through chelators (citric acid and EDTA). Cadmium altered morphological and physiological attributes of J. effusus as reflected by growth retardation. Citric acid in the presence of 100 μM Cd significantly countered Cd toxicity by improving plant growth. Elevated Cd concentrations reduced translocation factor that was increased under application of both chelators. Citric acid enhanced Cd accumulation, while EDTA reduced its uptake. Cadmium induced oxidative stress modified the antioxidative enzyme activity. Both levels of citric acid (2.5 and 5.0 mM) and lower EDTA concentration (2.5 mM) helped plants to overcome oxidative stress by enhancing their antioxidative enzyme activities. Cadmium damaged the root cells through cytoplasmic shrinkage and metal deposition. Citric acid restored structure and shape of root cells and eliminated plasmolysis; whereas, EDTA exhibited no positive effect on it. Shoot cells remained unaffected under Cd treatment alone or with citric acid except for chloroplast swelling. Only EDTA promoted starch accumulation in chloroplast reflecting its negative impact on cellular structure. It concludes that Cd and EDTA induce structural and morphological damage in J. effusus; while, citric acid ameliorates Cd toxicity stress.

Citric acid enhances the phytoextraction of manganese and plant growth by alleviating the ultrastructural damages in Juncus effusus L.

Chelate-assisted phytoextraction by high biomass producing plant species enhances the removal of heavy metals from polluted environments. In this regard, Juncus effusus a wetland plant has great potential. This study evaluated the effects of elevated levels of manganese (Mn) on the vegetative growth, Mn uptake and antioxidant enzymes in J. effusus. We also studied the role of citric acid and EDTA on improving metal accumulation, plant growth and Mn toxicity stress alleviation. Three-week-old plantlets of J. effusus were subjected to various treatments in the hydroponics as: Mn (50, 100 and 500 microM) alone, Mn (500 microM) + citric acid (5 mM), and Mn (500 microM) + EDTA (5 mM). After 2 weeks of treatment, higher Mn concentrations significantly reduced the plant biomass and height. Both citric acid and EDTA restored the plant height as it was reduced at the highest Mn level. Only the citric acid (but not EDTA) was able to recover the plant biomass weight, which was also obvious from the microscopic visualization of mesophyll cells. There was a concentration dependent increase in Mn uptake in J. effusus plants, and relatively more deposition in roots compared to aerial parts. Although both EDTA and citric acid caused significant increase in Mn accumulation; however, the Mn translocation was enhanced markedly by EDTA. Elevated levels of Mn augmented the oxidative stress, which was evident from changes in the activities of antioxidative enzymes in plant shoots. Raised levels of lipid peroxidation and variable changes in the activities of antioxidant enzymes were recorded under Mn stress. Electron microscopic images revealed several modifications in the plants at cellular and sub-cellular level due to the oxidative damage induced by Mn. Changes in cell shape and size, chloroplast swelling, increased number of plastoglobuli and disruption of thylakoid were noticed. However, these plants showed a high degree of tolerance against Mn toxicity stress, and it removed substantial amounts of Mn from the media. The EDTA best enhanced the Mn uptake and translocation, while citric acid best recovered the plant growth.

Enhancing crop growth, nutrients availability, economics and beneficial rhizosphere microflora through organic and biofertilizers

Field experiment was conducted on fodder maize to explore the potential of integrated use of chemical, organic and biofertilizers for improving maize growth, beneficial microflora in the rhizosphere and the economic returns. The treatments were designed to make comparison of NPK fertilizer with different combinations of half dose of NP with organic and biofertilizers viz. biological potassium fertilizer (BPF), Biopower, effective microorganisms (EM) and green force compost (GFC). Data reflected maximum crop growth in terms of plant height, leaf area and fresh biomass with the treatment of full NPK; and it was followed by BPF+full NP. The highest uptake of NPK nutrients by crop was recorded as: N under half NP+Biopower; P in BPF+full NP; and K from full NPK. The rhizosphere microflora enumeration revealed that Biopower+EM applied along with half dose of GFC soil conditioner (SC) or NP fertilizer gave the highest count of N-fixing bacteria (Azotobacter, Azospirillum, Azoarcus andZoogloea). Regarding the P-solubilizing bacteria,Bacillus was having maximum population with Biopower+BPF+half NP, andPseudomonas under Biopower+EM+half NP treatment. It was concluded that integration of half dose of NP fertilizer with Biopower+BPF / EM can give similar crop yield as with full rate of NP fertilizer; and through reduced use of fertilizers the production cost is minimized and the net return maximized. However, the integration of half dose of NP fertilizer with biofertilizers and compost did not give maize fodder growth and yield comparable to that from full dose of NPK fertilizers.

Bio-conversion of organic wastes for their recycling in agriculture : an overview of perspectives and prospects

Largely accessible organic wastes can be turned into valuable compost product for raising crops organically on one hand, and get them disposed off safely at the other end. Straight use of organic wastes has tribulations like transportation and handling, wider C:N ratio, high application rates, nutrient overloading, weed seeds, pathogens, and metal toxicities. Composting bestows a tactic for coping high volumes of organic wastes in environmentally sound and desirable manners. Composted materials are remarkably regarded for their ability to improve soil health and plant growth, and suppress pathogens and plant diseases. Currently several composting systems have become available; ranging from a crude and slow windrows method, to the most speedy and computer monitored in-vessel system. Scientific investigations of this biological cum chemical process have reached to molecular level. Value addition of compost through beneficial microorganisms, mineral materials and fertilisers is also being considered. The nature and composition of materials put into composting is imperative for its quality rationale. On the whole, principles and processes governing composting are not so straightforward that ordinary enterprises could develop efficient composting facilities for the treatment of organic wastes. In this scenario, accessibility of comprehensive information to the scientific community as well as environmental protection agencies is imperative. This review article brings together the current information necessary for effective composting of organic wastes from different origins with diversified characteristics under various situations. It also covers the schematic description of well known composting systems, and various factors controlling the process.

Allelochemicals : sources, toxicity and microbial transformation in soil -a review

Soil microorganisms interact with plants in diversified manner ranging from mobilising nutrients and enhancing their growth, to inducing diseases. They also produce allelochemicals directly or indirectly through conversion from other compounds. In order to hamper plant growth, allelochemicals must accumulate and persist at phytotoxic levels in the rhizosphere soil. However, after their entry into environment, persistence, availability and biological activities of allelochemicals are influenced by microorganisms. Transformation of allelochemicals by soil microbes may result into the compounds with modified biological properties. Such bio-transformations affect the overall allelopathic capability of the producer plant in a direct manner. Several reports describe the allelopathic significance of microbial metabolism products. For instance, a bacteriumActinetobacter calcoaceticus, can convert 2 (3H)-benzoxazolinone (BOA) to 2,2′-oxo-l,l′-azobenzene (AZOB) which is more inhibitory to some plants. On the contrary, bacteriumPseudomonas putida catabolises juglone in soils beneath walnut trees; otherwise, juglone accumulates at phytotoxic levels. This review article describes the nature of microbially produced allelochemicals, and the ways to mediate microbial degradation of putative allelochemicals. The given information develops an understanding of persistence, fate and phytotoxicity of allelochemicals in the natural environment, and also points out the possible solution of the problems due to microbial interventions in the soil.

Economizing the use of nitrogen fertilizer in wheat production through enriched compost

Manipulation of organic wastes and their composts as a source of organic matter (OM) and nutrients is imperative for sustainable agriculture. Further, the fortification of composts with chemical fertilizer enhances agronomic effectiveness of both by reducing the amount of fertilizer and improving the quality of compost. The present study aimed to explore the potential of organic and chemical nutrient sources with their optimal application and integration for sustainable wheat production. Accordingly, waste fruits and vegetables were collected, dried, ground and processed in a composting vessel. During the enriched composting, waste material (300 kg) was fortified with 30 kg N, i.e. 25% of the standard rate (120 kg N ha −1 ) of N fertilizer. Treatments for both greenhouse and field experiments using wheat ( Triticum aestivum L.) included: control (without any compost or N fertilizer), compost (non-enriched), fertilizer N 120 (120 kg N ha −1 ), nitrogen-enriched compost (NEC), NEC+N 30 (30 kg N ha −1 ) and NEC+N 60 (60 kg N ha −1 ). Application rate of composts (non-enriched or enriched) was 300 kg ha −1 in the respective treatments. Phosphorus and potassium fertilizers were applied at 90 kg P 2 O 5 ha −1 and 60 kg K 2 O ha −1 , respectively in all treatments. The crop was grown to maturity, and data on wheat growth and yield attributes were recorded. Application of NEC significantly improved the growth, yield and N, P and K contents of wheat compared with compost and control treatments. The performance of NEC+N 60 was statistically similar to that of fertilizer N 120. Economic analysis also revealed the superiority of NEC+N 60 over other treatments in terms of net return and relative increase in income; however, the value/cost ratio was highest with NEC alone. For effective and economical use of N fertilizer, it is suggested to integrate N fertilizer at reduced rates with NEC. Through enriched compost, application rates can be decreased from tonnes to kilograms per hectare, and dependence on chemical fertilizer can be reduced to a certain extent. So the approach is farmer friendly as it lowers compost application rates, and is economically acceptable as it saves N fertilizer. It is also environmentally sustainable due the recycling of organic waste and possible reduction of N losses to the environment. Thus, the study has wide application in the global environment and fertilizer market.

Isolation of Ochrobactrum sp.QZ2 from sulfide and nitrite treatment system

A bacterial strain QZ2 was isolated from sludge of anoxic sulfide-oxidizing (ASO) reactor. Based on 16S rDNA sequence analysis and morphology, the isolate was identified as Ochrobactrum sp. QZ2. The strain was facultative chemolithotroph, able of using sulfide to reduce nitrite anaerobically. It produced either elemental sulfur or sulfate as the product of sulfide oxidation, depending on the initial sulfide and nitrite concentrations. The optimum growth pH and temperature for Ochrobactrum sp. QZ2 were found as 6.5-7.0 and 30 degrees C, respectively. The specific growth rate (micro) was found as 0.06 h(-1) with a doubling time of 19.75h; the growth seemed more sensitive to highly alkaline pH. Ochrobactrum sp. QZ2 catalyzed sulfide oxidation to sulfate was more sensitive to sulfide compared with nitrite as indicated by IC(50) values for sulfide and nitrite utilization implying that isolate was relatively more tolerant to nitrite. The comparison of physiology of Ochrobactrum sp. QZ2 with those of other known sulfide-oxidizing bacteria suggested that the present isolate resembled to Ochrobactrum anthropi in its denitrification ability.

Model AVSWAT apropos of simulating non-point source pollution in Taihu lake basin

Accelerated eutrophication and nutrient loads in the lakes are of major concern for human health and environment. This study was undertaken for modeling the non-point source pollution of Taihu lake basin in eastern China. The SWAT model having an interface in ArcView GIS was employed. Model sensitive parameters related to hydrology and water quality were obtained by sensitivity analysis, and then calibrated and validated by comparing model predictions with field data. The GIS showed good potential for parameterization of hill-slopes, channels, and representative slope profiles for SWAT model simulations. In a monthly and daily time step, the models Nash-Sutcliffe coefficient (E) and the coefficient of determination (R(2)) indicated that values of simulated runoff, NH(4)(+)-N and total phosphorus were acceptably closer to the measured data. Surface water parameters especially CN, Soil-AWC and ESCO were the most sensitive and had more recognition in the model. It is concluded that runoff carrying N and P nutrients from chemical fertilizer inputs in agricultural areas is the major contributor to NPSP in the lake basin. So, decrease in excessive use of N and P fertilizers and their synergism with organic manures is recommended that would significantly reduce nutrient pollution in the lake ecosystem.

Optimization of struvite crystallization protocol for pretreating the swine wastewater and its impact on subsequent anaerobic biodegradation of pollutants

Higher contents of NH(4)(+) and SS in wastewater hamper the anaerobic digestion; necessitating its pretreatment to reduce them. This study reveals optimization of struvite/MAP precipitation protocol followed by anaerobic digestion of pretreated swine wastewater for pollutants removal. Levels of different treatments: stirring speeds, 400 and 160 rpm; pH values, 9.0, 9.5, 10.0, 10.5, 11.0 and 11.5; and P:Mg:N ratios, 1:1:1.2, 1:1:1.7, 1:1:2.2, 1:1:2.7, 1:1:4.0 and 1:1:5.0 were evaluated for MAP crystallization. Among various combinations, protocol comprising of initial 10 min stirring at 400 rpm followed by 160 rpm for 30 min, pH 10.0, and P:Mg:N ratio 1:1:1.2 rendered the best removal efficiency for NH(4)(+), PO(4)(3-), COD, TC and TOC. Subsequent anaerobic biodegradation revealed superiority of MAP supernatant over raw swine wastewater for methane yield and NH(4)(+)-N, PO(4)(3-)-P, COD, TC and TOC removals. It suggests that struvite precipitation as pretreatment to anaerobic digestion is highly effective and advantageous in wastewater treatment.

Isolation and characterization of Pseudomonas stutzeri QZ1 from an anoxic sulfide-oxidizing bioreactor

Bacterial strain QZ1 was isolated from sludge of anoxic sulfide-oxidizing (ASO) reactor. Based on 16S rDNA sequence analysis and morphological characteristics, the isolate was identified as Pseudomonas stutzeri. The isolate was found to be a facultative chemolithotroph, using sulfide as electron donor and nitrite as electron acceptor. The strain QZ1 produced sulfate as the major product of sulfide oxidation, depending on the initial sulfide and nitrite concentrations. The isolate was capable of growth under strictly autotrophic conditions. The growth and substrate removal of Pseudomonas stutzeri QZ1 were optimal at an initial pH of 7.5-8.0 at 30 degrees C. The specific growth rate (mu) was found as 0.035 h(-1) with a doubling time of 21.5 h. For isolate QZ1, the EC(50) values both for sulfide and nitrite were found to be 335.95 mg S L(-1) and 512.38 mg N L(-1), respectively, showing that the sulfide oxidation into sulfate by Pseudomonas stutzeri QZ1 was badly affected beyond these substrate concentrations.