Ghulam Shabir
National Institute for Biotechnology and Genetic Engineering
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Publication
Featured researches published by Ghulam Shabir.
Molecules | 2011
Ghulam Shabir; Farooq Anwar; Bushra Sultana; Zafar M. Khalid; Muhammad Afzal; Qaiser M. Khan; M. Ashrafuzzaman
This paper describes the antioxidant and antimicrobial activities and phenolic components of different solvent (absolute methanol, absolute ethanol, absolute acetone, 80% methanol, 80% ethanol, 80% acetone and deionized water) extracts of leaves, flowers and bark of Gold Mohar [Delonix regia (Bojer ex Hook.) Raf.]. The extract yields from leaves, flowers and bark ranged from 10.19 to 36.24, 12.97 to 48.47 and 4.22 to 8.48 g/100 g dry weight (DW), respectively. Overall, 80% methanol extract produced from the leaves exhibited significantly (P < 0.05) higher antioxidant activity, with high phenolic contents (3.63 g GAE/100 g DW), total flavonoid contents (1.19 g CE/100 g DW), inhibition of peroxidation (85.54%), DPPH scavenging capacity (IC50 value 8.89 μg/mL) and reducing power (1.87). Similarly, this 80% methanol leaves extract also showed superior antimicrobial activity. HPLC analysis of the 80% methanol extracts for individual phenolics revealed the presence of gallic, protocatechuic and salicylic acid in leaves; gallic, protocatechuic, salicylic, trans-cinnamic and chlorogenic acid in flowers, and gallic acid in bark as the main (amount > 1.50 mg/100 g DW) phenolic acids. Besides, small amounts (<1.50 mg/100 g DW) of some other phenolic acids such as sorbic, sinapic, p-coumaric, m-coumaric, ferulic, caffeic, 3-hydroxybenzoic, 4-hydroxycinnamic and 4-hydroxybenzoic acids were also detected. The extracts of the tested parts of Gold Mohar, especially, the leaves, might be valuable for functional food and therapeutic applications.
Frontiers in Plant Science | 2015
Muhammad Umar Khan; Angela Sessitsch; Muhammad Harris; Kaneez Fatima; Asma Imran; Muhammad Arslan; Ghulam Shabir; Qaiser M. Khan; Muhammad Afzal
Prosopis juliflora is characterized by distinct and profuse growth even in nutritionally poor soil and environmentally stressed conditions and is believed to harbor some novel heavy metal-resistant bacteria in the rhizosphere and endosphere. This study was performed to isolate and characterize Cr-resistant bacteria from the rhizosphere and endosphere of P. juliflora growing on the tannery effluent contaminated soil. A total of 5 and 21 bacterial strains were isolated from the rhizosphere and endosphere, respectively, and were shown to tolerate Cr up to 3000 mg l−1. These isolates also exhibited tolerance to other toxic heavy metals such as, Cd, Cu, Pb, and Zn, and high concentration (174 g l−1) of NaCl. Moreover, most of the isolated bacterial strains showed one or more plant growth-promoting activities. The phylogenetic analysis of the 16S rRNA gene showed that the predominant species included Bacillus, Staphylococcus and Aerococcus. As far as we know, this is the first report analyzing rhizo- and endophytic bacterial communities associated with P. juliflora growing on the tannery effluent contaminated soil. The inoculation of three isolates to ryegrass (Lolium multiflorum L.) improved plant growth and heavy metal removal from the tannery effluent contaminated soil suggesting that these bacteria could enhance the establishment of the plant in contaminated soil and also improve the efficiency of phytoremediation of heavy metal-degraded soils.
Molecules | 2012
Zahid Iqbal Sajid; Farooq Anwar; Ghulam Shabir; Ghulam Rasul; Khalid M. Alkharfy; Anwarul Hassan Gilani
This study appraises the antioxidant and antimicrobial attributes of various solvent extracts (absolute methanol, aqueous methanol, absolute ethanol, aqueous ethanol, absolute acetone, aqueous acetone, and deionized water) from bark, leaves and seeds of Pongamia pinnata (L.) Pierre. Maximum extraction yield of antioxidant components from bark (16.31%), leaves (11.42%) and seeds (21.51%) of P. pinnata was obtained using aqueous methanol (20:80). Of the extracts tested, the bark extract, obtained with aqueous methanol, exhibited greater levels of total phenolics [6.94 g GAE/100 g dry weight (DW)], total flavonoids (3.44 g CE/100 g DW), inhibition of linoleic acid peroxidation (69.23%) and DPPH radical scavenging activity (IC50 value, 3.21 μg/mL), followed by leaves and seeds extracts. Bark extract tested against a set of bacterial and fungal strains also revealed the strongest antimicrobial activity with the largest inhibition zone and lowest minimum inhibitory concentration (MIC). HPLC analysis of aqueous methanol extracts from bark, leaves and seeds indicated the presence of protocatechuic, ellagic, ferulic, gallic, gentisic, 4-hydroxybenzoic and 4-hydroxycinnamic acids in bark (1.50–6.70 mg/100 g DW); sorbic, ferulic, gallic, salicylic and p-coumaric acids in leaves (1.18–4.71 mg/100 g DW); vanillic, gallic and tannic acids in seeds (0.52–0.65 mg/100 g DW) as the main phenolic acids. The present investigation concludes that the tested parts of P. pinnata, in particular the bark, have strong potential for the isolation of antioxidant and antimicrobial agents for functional food and pharmaceutical uses.
Bioresource Technology | 2008
Muhammad Afzal; Ghulam Shabir; Irshad Hussain; Zafar M. Khalid
Pilot scale reactor based on combined biological-coagulation-filtration treatments was designed and evaluated for the treatment of effluent from a paper and board mill. Biological treatment by fed batch reactor (FBR) followed by coagulation and sand filtration (SF) resulted in a total COD and BOD reduction of 93% and 96.5%, respectively. A significant reduction in both COD (90%) and BOD (92%) was also observed by sequencing batch reactor (SBR) process followed by coagulation and filtration. Untreated effluent was found to be toxic, whereas the treated effluents by either of the above two processes were found to be non-toxic when exposed to the fish for 72h. The resultant effluent from FBR-coagulation-sand filtration system meets National Environmental Quality Standards (NEQS) of Pakistan and can be discharged into the environment without any risks.
Pedosphere | 2016
Ghulam Shabir; Muhammad Arslan; Kaneez Fatima; Imran Amin; Qaiser M. Khan; Muhammad Afzal
Abstract The combined use of plants and bacteria is a promising approach for the remediation of soil contaminated with organic pollutants. Different biotic and abiotic factors can affect the survival and activity of the applied bacteria and consequently plant growth and phytoremediation efficiency. The effect of inoculum density on the abundance and expression of alkane-degrading genes in the rhizosphere of plant vegetated in hydrocarbon-contaminated soil has been rarely observed. In this study, an alkane-degrading bacterium ( Pantoea sp. strain BTRH79), at different inoculum densities (10 5 to 10 8 cells cm −3 soil), was inoculated to ryegrass ( Lolium perenne ) vegetated in diesel-contaminated soil to find the optimum inoculum density needed for its efficient colonization and hydrocarbon degradation activity. Bacterial inoculation improved plant growth and hydrocarbon degradation. Maximum plant growth and hydrocarbon degradation were observed with the inoculum having the highest cell density (10 8 cells cm −3 soil). Moreover, the inoculum with higher cell density exhibited more abundance and expression of alkane hydroxylase gene, CYP153 . This study suggests that the inoculum density is one of the main factors that can affect bacterial colonization and activity during phytoremediation.
International Biodeterioration & Biodegradation | 2008
Ghulam Shabir; Muhammad Afzal; Farooq Anwar; Razia Tahseen; Zafar M. Khalid
Ecological Engineering | 2015
Amna Ijaz; Ghulam Shabir; Qaiser M. Khan; Muhammad Afzal
Clean-soil Air Water | 2014
Muhammad Afzal; Ghulam Shabir; Samina Iqbal; Tanveer Mustafa; Qaiser M. Khan; Zafar M. Khalid
Clean-soil Air Water | 2014
Muhammad Afzal; Ghulam Shabir; Razia Tahseen; Ejazul Islam; Samina Iqbal; Qaiser M. Khan; Zafar M. Khalid
International Biodeterioration & Biodegradation | 2016
Razia Tahseen; Muhammad Afzal; Samina Iqbal; Ghulam Shabir; Qaiser M. Khan; Zafar M. Khalid; Ibrahim M. Banat
Collaboration
Dive into the Ghulam Shabir's collaboration.
National Institute for Biotechnology and Genetic Engineering
View shared research outputsNational Institute for Biotechnology and Genetic Engineering
View shared research outputsNational Institute for Biotechnology and Genetic Engineering
View shared research outputsNational Institute for Biotechnology and Genetic Engineering
View shared research outputsNational Institute for Biotechnology and Genetic Engineering
View shared research outputsNational Institute for Biotechnology and Genetic Engineering
View shared research outputsNational Institute for Biotechnology and Genetic Engineering
View shared research outputs