Giana de Paula Cognato
Universidade Federal do Rio Grande do Sul
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Publication
Featured researches published by Giana de Paula Cognato.
International Journal of Developmental Neuroscience | 2005
Giana de Paula Cognato; Alessandra Nejar Bruno; Fernanda Cenci Vuaden; João José Freitas Sarkis; Carla Denise Bonan
Adenosine, a well‐known neuromodulator, can act as an endogenous anticonvulsant via the activation of adenosine A1 receptors. This adenine nucleoside can be produced in the synaptic cleft by the ectonucleotidase cascade, which includes the nucleoside triphosphate diphosphohydrolase (NTPDase) family and ecto‐5′‐nucleotidase. It has been previously reported that ectonucleotidase activities are increased in female adult rats submitted to the pilocarpine model of epilepsy. Several studies have suggested that the immature brain is less vulnerable to morphologic and physiologic alterations after status epilepticus (SE). Here, we evaluate the ectonucleotidase activities of synaptosomes from the hippocampus and cerebral cortex of male and female rats at different ages (7–9, 14–16 and 27–30‐day old) submitted to the pilocarpine model of epilepsy. Our results show that ATP and ADP hydrolysis in the hippocampus and cerebral cortex were not altered by the pilocarpine treatment in female and male rats at 7–9, 14–16 and 27–30 days. There were no changes in AMP hydrolysis in female and male rats submitted to the model at different ages, but a significant increase in AMP hydrolysis (71%) was observed in synaptosomes from the cerebral cortex of male rats at 27–30 days. Pilocarpine‐treated male rats (60–70‐day old) presented an enhancement in ectonucleotidase activities in the synaptosomes of the cerebral cortex (33, 40 and 64% for ATP, ADP and AMP hydrolysis, respectively) and hippocampus (55, 98 and 101% for ATP, ADP and AMP hydrolysis, respectively). These findings highlight differences between the purinergic system of young and adult rats submitted to the pilocarpine model of epilepsy.
International Journal of Developmental Neuroscience | 2008
Giana de Paula Cognato; Rafael Sanguinetti Czepielewski; João José Freitas Sarkis; Maurício Reis Bogo; Carla Denise Bonan
Ecto‐nucleotide pyrophosphatases/phosphodiesterases (E‐NPPs) are membrane‐bound ecto‐enzymes involved in the modulation of purinergic signaling. Important physiological roles related to brain development have been associated to purinergic neurotransmission. NPP1, two splice isoforms of NPP2, and NPP3 have already been identified in adult rat brain. However, there are no studies evaluating the mRNA expression of these NPP members during the brain development. The effort of the present study was to map NPP gene expression pattern in olfactory bulb, hippocampus, cerebral cortex, striatum, and cerebellum at crucial ages for rat development (7, 14, 21, 60, and 150 days old) by a semi‐quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR) strategy. Our results demonstrated an increase in the relative expression of NPP1 throughout the aging in all structures analyzed, except in hippocampus, where the higher expression has been detected in 14 days old rats. Both NPP2 isoforms have shown a similar pattern of expression among all structures. The relative expression of NPP3 decreased during the aging mainly on cerebellum, hippocampus, and olfactory bulb. Altogether, the different patterns of NPP gene expression during rat brain development reinforce the idea that each enzyme may play a distinct role on modulating the purinergic signaling throughout aging.
Life Sciences | 2007
Fernanda Cenci Vuaden; Giana de Paula Cognato; Cristina Bonorino; Maurício Reis Bogo; João José Freitas Sarkis; Carla Denise Bonan
Epilepsy Research | 2007
Jean Pierre Oses; Giordano Gubert Viola; Giana de Paula Cognato; Victor Hermes Cereser Júnior; Gisele Hansel; Ana Elisa Böhmer; Renata Leke; Alessandra Nejar Bruno; Carla Denise Bonan; Maurício Reis Bogo; Luis Valmor Cruz Portela; Diogo O. Souza; João José Freitas Sarkis
Neurochemical Research | 2007
Giana de Paula Cognato; Alessandra Nejar Bruno; Rosane Souza da Silva; Maurício Reis Bogo; João José Freitas Sarkis; Carla Denise Bonan
Journal of Experimental Zoology Part A: Comparative Experimental Biology | 2004
Eliane Borges; Fernanda Cenci Vuaden; Giana de Paula Cognato; Maria da Graça Fauth; Carla Denise Bonan; Guendalina Turcato; Isabel Cristina Rossi; Renato D. Dias
Neuroscience | 2011
Giana de Paula Cognato; Fernanda Cenci Vuaden; L.E.B. Savio; B. Bellaver; Emerson André Casali; Maurício Reis Bogo; Diogo Onofre Gomes de Souza; Jean Sévigny; Carla Denise Bonan
The Open Neuroscience Journal | 2010
Giana de Paula Cognato; Carla Denise Bonan
Neurochemical Research | 2006
Patrícia Pelufo Silveira; Giana de Paula Cognato; Leonardo Machado Crema; F. Q. Pederiva; Carla Denise Bonan; João José Freitas Sarkis; Aldo Bolten Lucion; Carla Dalmaz
Metabolic Brain Disease | 2010
Vanessa Gass da Silveira; Giana de Paula Cognato; Alexandre Pastoris Müller; Fabrício Figueiró; Carla Denise Bonan; Marcos Luiz Santos Perry; Ana Maria Oliveira Battastini