Giancarlo Colelli

Factors Affecting Quality and Safety of Fresh-Cut Produce

The quality of fresh-cut fruit and vegetable products includes a combination of attributes, such as appearance, texture, and flavor, as well as nutritional and safety aspects that determine their value to the consumer. Nutritionally, fruit and vegetables represent a good source of vitamins, minerals, and dietary fiber, and fresh-cut produce satisfies consumer demand for freshly prepared, convenient, healthy food. However, fresh-cut produce deteriorates faster than corresponding intact produce, as a result of damage caused by minimal processing, which accelerates many physiological changes that lead to a reduction in produce quality and shelf-life. The symptoms of produce deterioration include discoloration, increased oxidative browning at cut surfaces, flaccidity as a result of loss of water, and decreased nutritional value. Damaged plant tissues also represent a better substrate for growth of microorganisms, including spoilage microorganisms and foodborne pathogens. The risk of pathogen contamination and growth is one of the main safety concerns associated with fresh-cut produce, as highlighted by the increasing number of produce-linked foodborne outbreaks in recent years. The pathogens of major concern in fresh-cut produce are Listeria monocytogenes, pathogenic Escherichia coli mainly O157:H7, and Salmonella spp. This article describes the quality of fresh-cut produce, factors affecting quality, and various techniques for evaluating quality. In addition, the microbiological safety of fresh-cut produce and factors affecting pathogen survival and growth on fresh-cut produce are discussed in detail.

Effect of atmosphere composition on the quality of ready-to-use broccoli raab (Brassica rapa L.)

BACKGROUND Many leafy brassicas are widely used for ready-to-use salads. Broccoli raab (Brassica rapa L.), also called turnip top, or rapini, is extensively cultivated in southern Italy. The edible portion is made up of the green, immature inflorescences and the stem with its most tender leaves. Recently, interest in this crop has increased among European consumers; moreover, a substantial increase in consumption could come from the ready-to-use product. RESULTS The effects of four different atmosphere compositions (air, 3% O(2) in nitrogen, 3% O(2) + 10% CO(2) in air, and 10% CO(2) in air) on quality attributes of ready-to-use broccoli raab were studied. Controlled atmosphere affected appearance, composition, respiration rate, weight loss, and presence of off-odours. Storage of broccoli raab florets under low oxygen conditions delayed post-cutting deterioration during storage at 5 degrees C and preserved appearance and typical odour, up to 17 days. Moreover, respiration rate as well as loss of green colour, chlorophyll and vitamin C were also slowed down in this condition. Finally, in this study no effect of controlled atmosphere storage was found on total phenols content and antioxidant activity. CONCLUSION Results showed that cold storage in 3% O(2) can be beneficial in order to maintain quality of ready-to-use, broccoli raab for up to 17 days.

Potential use of microwave treatment on fresh-cut carrots: physical, chemical and microbiological aspects.

BACKGROUND The effect of microwave treatments (900 and 750 W for 45 and 60 s) on the microbial, physicochemical and sensory properties of fresh-cut carrot slices and the contents of several bioactive compounds was studied. Carrot samples were stored for 7 days at 5 °C. RESULTS The microwaving of fresh-cut carrots reduced the initial respiration rate (8.6 CO2 mL kg(-1) h(-1)) by 55-74% compared with untreated samples, although the rates then increased during storage. The initial pH (6.7), titratable acidity (0.036%), soluble solid content (8.2 °Brix) and shelf-life of the samples did not differ greatly from those of the untreated samples. Microwaving prevented the incipient whitening and surface dryness during storage. In general, no significant changes in phenylalanine ammonia lyase activity (5.5 µmol t-cinnamic acid kg(-1) h(-1)), total phenolics (TP, 81.3 mg chlorogenic acid equivalent kg(-1) fresh weight (FW)) or total antioxidant capacity (TAC, 74.2 µmol Trolox equivalent kg(-1) FW) were observed on the processing day or over storage. However, the mildest treatment (750 W for 45 s) caused TP and TAC enhancements of 118 and 394% respectively after 7 days of shelf-life. Microwave treatments reduced the initial microbial loads of the samples by up to 1.8 log units, although their microbial growth was greater than that of the untreated samples throughout storage. CONCLUSION Mild microwave treatments such as 750 W/45 s and 750 W/60 s are a good sustainable alternative to the use of NaOCl; however, combining them with other sanitizing techniques is needed to control microbial growth throughout the shelf-life of fresh-cut carrot slices.

Effect of harvest time on table grape quality during on-vine storage

BACKGROUND Postponing the harvest of grapes is a common practice in southern Italy, in order to delay harvest up to Christmas and make higher income from their sale. The aim of this work was to evaluate the effect of harvest time (over almost 3 months) on the quality of table grapes (cv. Italia). The experiment was repeated for two years (2010 and 2011). In 2010, grapes were harvested starting from 8 October and after 11, 27 and 48 days. In 2011, five harvest times were compared over a period of 56 days. Respiration rate, firmness, colour, sensory attributes, total soluble solids (TSS), pH, titratable acidity (TA), phenols and antioxidant activity were measured. In addition, in the second year, volatile compounds were evaluated. RESULTS For both years, harvest time influenced most parameters, which indicated that metabolic changes took place in the plants. In 2010, harvest time influenced respiration rate, cluster and berry appearance scores, colour attributes, crunchiness, pH, TA, total phenol content and antioxidant activity. In 2011, harvest time influenced respiration rate, colour attributes, most sensory attributes, TSS and TA. Generally, late harvested grapes showed higher firmness, berry appearance score, sweetness, fruity taste, overall sensory evaluation score and TSS. Regarding volatile compounds, terpene content decreased during ripening, while C6 compounds showed a nonlinear trend. CONCLUSION The results showed that table grape sensory quality could be increased by delaying harvest up to a certain time of the season, while excessive delay could reduce final grape quality.

The table grape ‘Victoria’ with a long shaped berry: a potential mutation with attractive characteristics for consumers

BACKGROUND Puglia is the most important region in Italy for table grape production. Since consumers look for new products, the number of table grape varieties has greatly increased in recent years. RESULTS In a survey in the Puglia region, we identified several years ago a potential mutation of the cv. Victoria. We described this accession in comparison with the standard Victoria for some amphelographic traits. All the characteristics were very similar to the standard Victoria except for the berry shape, which was significantly more elongated. Moreover, the berry of the mutated Victoria showed higher firmness, lightness and chroma than the standard one, with a more intense yellow colour of the skin (appreciated by consumers). The molecular characterisation with 25 SSR markers showed that normal and mutant Victoria were genetically identical at all the analysed loci, thus suggesting that the two accessions could be considered as clones with the difference in berry shape probably due to a somatic mutation. CONCLUSIONS This mutation of the cv. Victoria may have interesting perspective for the market since consumers are always attracted by different shape and colour of the fruits (consumers buy with eyes). This accession can be an alternative clone of the already known standard Victoria.

PCR-based Quality Control of Listeria spp. and L. monocytogenes in Ready-To-Eat Salads Sold in Italy

A total of 48 samples of ready to-eat salads (RTE), containing different varieties of salads, of different brands sold in Italy, were purchased from several supermarkets, during period 2008 – 2009. This study was performed to determine whether microbial contamination, and in particular pathogenic bacteria, such as Listeria monocytogenes, were present in these food products. Samples were examined for mesophilic aerobic counts, yeasts and moulds, total and fecal coliforms, and presumptive Listeria monocytogenes counts. Results showed that, in general, the highest microorganism counts were associated with samples containing grated carrot (6.3 Log cfu g-1 of aerobic mesophilic microorganisms; 4.6 Log cfu g-1 of yeast and moulds; 2.8 Log cfu g-1 of total coliforms), while the lowest counts were, generally, associated with samples containing lettuce (2.8 Log cfu g-1 of aerobic mesophilic microorganisms; 2.3 Log cfu g-1 of yeast and moulds; 2.3 Log cfu g-1 of total coliforms). Of all the samples analyzed, only three (6 %) harboured L. monocytogenes.

Quality of fresh-cut products as affected by harvest and postharvest operations

There is a rising demand for fresh-cut convenience products with high quality and nutritional standards that needs to be met by the fresh-cut industry. It is well known that harvest and postharvest handling of fresh produce has a paramount impact on its quality and storage, although most of the existing literature has focused on these impacts related only to fresh produce that is destined for the final consumers. Indeed, current harvest methods and postharvest technologies have improved fruit and vegetable handling and distribution processes by slowing down physiological processes and senescence. Nonetheless, these technologies and methods may influence the quality of fresh produce as raw material for fresh-cut processing as a result of the dynamic responses of fresh produce to handling procedures and treatments. Here, we review the existing literature on the challenges facing the fresh-cut industry, focusing on the impact of harvest, maturity, and handling of fruit and vegetables on the quality of raw materials, as well as the implications for fresh-cut products. The review also highlights areas for further research with the aim of enhancing the sensorial, nutritional and biochemical quality of such products.

Effect of organic agronomic techniques and packaging on the quality of lamb's lettuce: Organically produced lamb's lettuce: quality and post-harvest storage

BACKGROUND This research focused on the effect of organic production systems on initial quality and postharvest performance of lambs lettuce leaves stored in air or under modified atmosphere at refrigerated temperature. Different strategies of organic soil fertility management were compared under the same environmental conditions: (i) a simplified organic production system based on organic commercial fertilizers to recover crop uptake (SB); (ii) an organic production system based on organic matter amendment mainly supplied by animal manure (AM); and (iii) an organic production system based on organic matter amendment supplied by green waste compost (AC). Fully developed lambs lettuce leaves were harvested and then packed into perforated bags (control in AIR) or in modified atmosphere packaging (MAP) and stored at 4 °C. RESULTS At harvest, the yield of lambs lettuce in the AM and AC systems was higher than that in SB. Phenol and dehydroascorbic acid accumulation was observed in the system with the lowest initial supply of organic amendment (SB). Regarding the effect of packaging, AIR conditions maintained the initial quality attributes for a longer period than MAP, which developed off-odours above the threshold of acceptability at 11 days, irrespective of the production system used. CONCLUSION In general, the initial differences among the production systems were minimal during the postharvest storage. As for the tested packaging systems, AIR successfully maintained the initial quality attributes for a longer period than the MAP.