Gianni Pozzi
University of Cagliari
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Publication
Featured researches published by Gianni Pozzi.
Vaccine | 1997
Donata Medaglini; Catherine M. Rush; Piersante Sestini; Gianni Pozzi
There is a need to develop vaccines to control the spread of sexually transmitted diseases (STDs). Novel immunization strategies that elicit a mucosal immune response in the genital tract, may show improved protection by preventing or at least limiting entry of the pathogenic micro-organism. However, it has proven difficult to obtain a local immune response in the vaginal mucosa. Our approach is based on the use of recombinant bacteria capable of colonizing mucosal surfaces as live vaccine vectors. The human commensal Streptococcus gordonii, engineered to express the E7 protein of human papillomavirus type 16, was used for intravaginal immunization of mice. A single inoculum of recombinant bacteria was sufficient to establish colonization of the murine vagina and therefore induce papillomavirus-specific vaginal IgA and serum IgG. Evidence that mucosal colonization with recombinant commensal bacteria can induce a local immune response in the female genital tract represents a significant step toward the development of new vaccines against STDs.
Archive | 1997
Gianni Pozzi; Marco R. Oggioni; Donata Medaglini
To investigate the possible use of commensal bacteria as live vehicles for vaccine antigens, we chose as a model Streptococcus gordonii, a Gram-positive coccus, which is a normal constituent of the microbial flora of the human oral cavity.1,2 The strain “Challis” of S. gordonii, formerly classified as Streptococcus sanguis? allows easy genetic manipulation because it is naturally competent for genetic transformation.4,5 S. gordonii “Challis” was also found capable of colonizing the mouse oral and vaginal mucosa (see below), thus providing an animal model for testing the immunogenicity of the recombinant strains.
Archive | 1997
Catherine M. Rush; Annick Mercenier; Gianni Pozzi
The genus Lactobacillus comprises a remarkably diverse and heterogeneous group of Gram-positive bacilli that are ubiquitous as components of the normal indigenous flora of humans and other animals as well as being important lactic acid bacteria used for the production of and found naturally in fermented foods.1 Their “health-promoting” properties have been advocated since the early part of this century, this has resulted in their consumption in many countries as dietary adjuncts, in dairy products such as yogurts and in probiotic preparations.2–5 Administration of viable Lactobacillus strains has been described as therapeutic for diarrhea and other intestinal disorders, vaginitis and urinary tract infections. The mechanisms underlying this effect are not well understood, although they probably involve restoration of the natural balance between the normal microflora and the invading organism, which in turn results in elimination of the pathogen by the hosts’ immune system. Lactobacilli are rarely pathogenic, thus making them an excellent choice for consideration as vaccine delivery vehicles.
Plasmid | 1997
Riccardo Manganelli; Susanna Ricci; Gianni Pozzi
Archive | 1993
Donata Medaglini; Marco R. Oggioni; Mario Contorni; F. Cavalieri; Gianni Pozzi
Archive | 2010
Annalisa Ciabattini; Elena Pettini; Fabio Fiorino; Anna Maria Cuppone; Gennaro Prota; Gianni Pozzi; Donata Medaglini
Archive | 2011
Damiana Chiavolini; Guido Memmi; Tiziana Maggi; Francesco Iannelli; Gianni Pozzi; Marco R. Oggioni
Archive | 2008
Marco R. Oggioni; Gianni Pozzi; Susanna Ricci
Archive | 2005
Donata Medaglini; Annalisa Ciabattini; Anna Maria Cuppone; Caterina Costa; Susanna Ricci; Gianni Pozzi
ISTISAN CONGRESSI | 2002
Annalisa Ciabattini; Barbara Giomarelli; Tiziana Maggi; Donata Medaglini; Gianni Pozzi