Gilbert J. Hite

Alteration of in vivo and in vitro effects of heroin by esterase inhibition

Selective inhibition of peripheral esterases by tri-ortho-tolyl phosphate in the mouse resulted in an increase in the analgetic activity of heroin, without affecting the activity of morphine. In vitro inhibition of esterases by paraoxon reduced the affinity of heroin for the opiate receptor, while that of morphine was unaffected. These results suggest that both central and peripheral esterases are involved in the metabolism of heroin and that interference with critical esterases can alter its pharmacologic and toxicologic effects.

The Preparative Scale Reverse Phase HPLC Separation of Epimeric Alkaloids Using Camphorsulfonic Acid as an Ion Pairing Reagent

Abstract A reverse phase paired ion HPLC procedure is described for the separation of multigram quantities of epimeric alkaloids using camphorsulfonic acid as the ion-pairing reagent.